The fractal and textural analysis of glomeruli in obese and non-obese patients

Jordanova, Elena; Janković, Radmila; Naumovic, Radomir; Celic, Dejan; Ljubicic, Bojana; Simić-Ogrizović, Sanja; Basta-Jovanović, Gordana

doi:10.1016/j.jpi.2022.100108

Cited by 2 publications

(10 citation statements)

References 33 publications

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“…Low and high magnification shown. c-d, Mosaic assay of non-conditionally immortalized human podocytes cultivated on concave and convex HF scaffolds (n=3) for a period of 5 days and imaged by c, epifluorescence (seeding densities at 100,000 and 200,000 cells/cm 2 ) and d, confocal microscopy (seeding density at 100,000 cells/cm 2 ). e-f, Quantification of e, cell numbers and f, whole cell morphology by fractal dimension of Mosaic assay human podocytes cultivated on concave and convex HF scaffolds (n=3) for 5 days.…”

Section: Resultsmentioning

confidence: 99%

“…Positive photoresist AZ P4620 was then spin coated on top of the HMDS layer at 1500 rpm for 30 seconds, followed by softbaking at 110 °C for 80 seconds and rehydrating for approximately 30 minutes at room temperature. The design patterns were written onto the wafer by exposing the photoresist to laser at 770 mJ/cm 2 , followed by developing and reflow (120°C for 1-2 minutes) to smooth the edges of the features.…”

Section: Methodsmentioning

confidence: 99%

“…To induce differentiation, cells were cultured under the differentiation condition at 37 or 38°C in DMEM-F12 basal media with 10% FBS and 1% pen-strep. The immortalized Podo/Tert256 human podocyte hTERT cell line (Evercyte) was expanded in flasks coated with 6 µg/cm 2 human collagen I (Sigma, C7774) at 37°C in MCDB131 basal media (Gibco, #10372019) supplemented with 20% FBS, 2 mM GlutaMAX (Gibco, #35050061), 12 µg/mL Bovine Brain Extract (Lonza, CC-4098), 10 ng/mL hEGF (Sigma-Aldrich, E9644), 25 ng/mL hydrocortisone (Sigma-Aldrich, Cat# H0396), and 100 µg/mL G418 (InvivoGen, ant-gn-5). For both mouse and human podocytes, media was changed every 2-3 days.…”

Section: Methodsmentioning

confidence: 99%

“…YAP Inhibition Study : HF and NT scaffolds were prepared as described above. Mosaic human podocytes were seeded on top of the scaffolds at a concentration of 50,000 cells/cm 2 (100,000 cells per scaffold). Verteporfin (1 or 2 μg/ml, Tocris) was added to the culture media 24 hours post seeding.…”

Section: Methodsmentioning

confidence: 99%

“…As such, their morphology can be characterized by fractal dimension (Df), a ratio of the change in detail to the change in scale and often used for quantifying complex geometry. Similar to podocytes, a kidney glomerulus has a complex architecture, with fractal analysis performed on itself 2 or its components 3 under healthy and pathological/injury conditions. Not surprisingly, state of tissue health has been reported to correlate with their fractality, 4 which suggests engineering microenvironmental factors such as biophysical shape cues could control cell or tissue fate.…”

Section: Introductionmentioning

confidence: 99%

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Biomimetic fractal topography enhances podocyte maturation in vitro

Liu,

Aggarwal,

Wagner

et al. 2024

Preprint

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Cells and tissues in their native environment are organized into intricate fractal structures, which are rarely recapitulated in their culture in vitro. The extent to which fractal patterns that resemble complex topography in vivo influence cell maturation, and the cellular responses to such shape stimulation remain inadequately elucidated. Yet, the application of fractal cues (topographical stimulation via self-similar patterns) as an external input may offer a much-needed solution to the challenge of improving the differentiated cell phenotype in vitro. Here, we established fractality in podocytes, branching highly differentiated kidney cells, and glomerulus structure. Biomimetic fractal patterns derived from glomerular histology were used to generate topographical (2.5-D) substrates for cell culture. Podocytes grown on fractal topography were found to express higher levels of functional markers and exhibit enhanced cell polarity. To track morphological complexities of differentiated podocytes, we employed a fluorescent labelling assay where labelled individual cells are tracked within otherwise optically silent confluent cell monolayer to reveal cell-cell interdigitation. RNAseq analysis suggests enhanced ECM deposition and remodeling in podocytes grown on fractal topography compared to flat surface or non-fractal microcurvature, mediated by YAP. The incorporation of fractal topography into standard tissue culture well plates as demonstrated may serve as a user-friendly bioengineered platform for high-fidelity cell culture.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Biomimetic fractal topography enhances podocyte maturation in vitro

Liu,

Aggarwal,

Wagner

et al. 2024

Preprint

View full text Add to dashboard Cite

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A lung cancer risk warning model based on tongue images

et al. 2023

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Objective: To investigate the tongue image features of patients with lung cancer and benign pulmonary nodules and to construct a lung cancer risk warning model using machine learning methods.Methods: From July 2020 to March 2022, we collected 862 participants including 263 patients with lung cancer, 292 patients with benign pulmonary nodules, and 307 healthy subjects. The TFDA-1 digital tongue diagnosis instrument was used to capture tongue images, using feature extraction technology to obtain the index of the tongue images. The statistical characteristics and correlations of the tongue index were analyzed, and six machine learning algorithms were used to build prediction models of lung cancer based on different data sets.Results: Patients with benign pulmonary nodules had different statistical characteristics and correlations of tongue image data than patients with lung cancer. Among the models based on tongue image data, the random forest prediction model performed the best, with a model accuracy of 0.679 ± 0.048 and an AUC of 0.752 ± 0.051. The accuracy for the logistic regression, decision tree, SVM, random forest, neural network, and naïve bayes models based on both the baseline and tongue image data were 0.760 ± 0.021, 0.764 ± 0.043, 0.774 ± 0.029, 0.770 ± 0.050, 0.762 ± 0.059, and 0.709 ± 0.052, respectively, while the corresponding AUCs were 0.808 ± 0.031, 0.764 ± 0.033, 0.755 ± 0.027, 0.804 ± 0.029, 0.777 ± 0.044, and 0.795 ± 0.039, respectively.Conclusion: The tongue diagnosis data under the guidance of traditional Chinese medicine diagnostic theory was useful. The performance of models built on tongue image and baseline data was superior to that of the models built using only the tongue image data or the baseline data. Adding objective tongue image data to baseline data can significantly improve the efficacy of lung cancer prediction models.

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The fractal and textural analysis of glomeruli in obese and non-obese patients

Cited by 2 publications

References 33 publications

Biomimetic fractal topography enhances podocyte maturation in vitro

Biomimetic fractal topography enhances podocyte maturation in vitro

A lung cancer risk warning model based on tongue images

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