The Free Amino Acids of Bovine Semen

Hopwood, M. L.; Gassner, F. X.

doi:10.1016/s0015-0282(16)34510-1

Cited by 22 publications

(10 citation statements)

References 11 publications

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“…low concentration, low motility, and increase in dead and abnormal sperm (Taha et al, 2006). This finding coincided with those of Hopwood and Gassner (1962) who reported that increased concentration of seminal total free amino acids was related to the increased quality and fertility of semen, and that poor semen quality was related to amino acids imbalance. Furthermore, a quantitative reduction in the concentration of all seminal plasma free amino acids has been observed in cases of azoospermia (Chaudhury et al, 2002;Papp et al, 1983) and oligospermia (Silvestroni et al, 1979).…”

Section: Amino Acidssupporting

confidence: 84%

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Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Taha

Shaaban

El-Nouty

et al. 2008

Animal

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This study was done to evaluate the effects of two sublethal doses of gossypol (4 and 20 mg/kg of BW, every other day) on some amino and fatty acid concentrations in male rabbit seminal plasma. Rabbits were chosen as an experimental animal owing to the fact that they are excellent model for reproductive toxicological effects. The experiment lasted 16 weeks and included two periods: a treatment period (first 8 weeks) where the animals were given the tested product, and a recovery period (second 8 weeks) where all drugs were withdrawn. Results showed that total amino acids (TAA), total essential amino acids (EAA), total non-essential amino acids (non-EAA) and EAA/non-EAA ratio were decreased in a dose-dependent manner during gossypol treatment. The deleterious effect on TAA concentrations was mainly due to the reduction in total EAA. However, these concentrations regained their normal values after gossypol cessation. Basic, acidic, neutral amino acids and basic/acidic amino acids ratio decreased in a dose-dependent manner by gossypol treatment. Additionally, gossypol administration caused decreases in total unsaturated fatty acids (USFA) and increases in total saturated fatty acids (SFA) and the SFA/USFA ratio in a dose-dependent manner. During the recovery period, total SFA and USFA showed significant reduction and significant increase, respectively, after gossypol withdrawal. In conclusion, gossypol administration affected rabbit seminal plasma concentrations of amino and fatty acids in a dose-dependant manner. Gossypol reduced TAA, total EAA and total non-EAA. Additionally, gossypol caused decreases in total USFA and increases in total SFA. These deleterious effects were associated with poor-quality semen observed in our previous studies.

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Section: Amino Acidssupporting

confidence: 84%

“…The testes and epididymis are the major sources of seminal amino acids (Hopwood and Gassner, 1962). Gossypol administration was reported to cause testicular destruction (Arshami and Ruttle, 1988;Chase et al, 1994), which could impair seminal amino acid synthesis.…”

Section: Amino Acidsmentioning

confidence: 99%

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Taha

Shaaban

El-Nouty

et al. 2008

Animal

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“…The testicular origin of at least some of the amino acids in bull seminal plasma has been shown earlier (Gassner and Hopwood 1952;Hopwood and Gassner 1962) and, despite the contribution of the seminal vesicles, the concentration of most amino acids in the seminal plasma is not altered significantly by removal of these glands (Hess et al 1960). However, in the boar the seminal vesicles appear to contribute most of the glycine to the seminal plasma (Johnson et al 1972).…”

Section: Incubation Of Semenmentioning

confidence: 88%

“…Although there are several reports on the free amino acid composition of ram and bull semen and reproductive tract fluids (Gassner and Hopwood 1952;Bhargava et al 1959;Hopwood and Gassner 1962;Setchell et al 1967), no direct comparison has been made between the amino acid content of the following fluids: rete testis fluid, epididymal plasma, vesicular fluid, seminal plasma and blood plasma. There are also little data available on the amino acid composition of the reproductive tract fluids of the rat or human.…”

Section: Introductionmentioning

confidence: 99%

Amino Acid Composition of Semen and the Secretions of the Male Reproductive Tract

Pd¹,

White²

1974

Aust. Jnl. Of Bio. Sci.

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The free amino acid content of rete testis fluid, epididymal fluid, vesicular fluid and ejaculated seminal plasma was studied in the ram, bull and rat, and a comparison made with the blood plasma of the same species. The amino acid content of human seminal plasma and the various fractions of the boar ejaculate was also determined.Glutamic acid was found to be the predominant amino acid in the secretions of the reproductive tract of the ram and bull. It is present in high concentrations in the rete testis fluid of both species, and is further concentrated along the tract. In the rat glycine and alanine are the predominant amino acids in the rete testis fluid, while glutamic acid is the principal amino acid in epididymal fluid, as in the other species studied. Glycine is also the principal amino acid in human seminal plasma. The concentration of amino acids is greatest in the sperm-rich fraction of the boar ejaculate.The breakdown of protein to amino acid was studied in ram and bull semen. This is at least partly due to enzymically induced changes.

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“…Free amino acids may serve as oxidizable substrate for aerobic metabolism by spermatozoa (MANN, 1964), create the favourable conditions for nucleic acid synthesis (SETCHELL et al, 1967) or enhance sperm survival (TYLER and ROTHSCHILD, 1951). The free amino acid composition of bull semen has been reported (HOPWOOD and GASSNER, 1962;MANN, 1964; AL-HAKIM et al, 1970 and BROWN-WOODMAN and WHITE, 1974) but little information is available on the amino acid nitrogen and free amino acids of buffalo bull semen. The objective of this study was, therefore, to see the effect of different periods of the year on the nitrogenous constituents and free amino acid composition of buffalo bull seminal plasma.…”

Section: Introductionmentioning

confidence: 99%

Nitrogenous Constituents and Free Amino acid Content in the Seminal Plasma of Buffalo (Bos Bubalis) in Different Periods of the Year in Relation to Fertility

Chaudhary

Gangwar

2010

Zentralblatt Für Veterinärmedizin Reihe A

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Summary Semen of eight Murrah buffalo bulls was analysed for different nitrogenous constituents in different periods of the year and for the free amino acid content of seminal plasma. Total nitrogen, protein nitrogen, non‐protein nitrogen and amino acid nitrogen were found to be 649.10 ± 12.83, 532 ± 11.56, 116.26 ± 2 and 99.13 ± 4.4 mg. / 100 ml., of seminal plasma. All the nitrogen fractions vary significantly (P ≤ 0.01) between the bulls and between the periods. All the nitrogen fractions tended to be higher in colder periods as compared to the hot ones. Protein contributed 82 % of the total nitrogen. Conception rate was lowest during the hot period. NPN and amino acid nitrogen had a positive correlation with conception rate. Ten free amino acid‐aspartic acid, glutamic acid, serine, alanine, glycine, lysine, arginine, histidine and tyrosine (tryptophan) were identified by two dimensional TLC. Glutamic acid was the predominant amino acid (59.78 %) followed by glycine, serine, alanine and aspartic acid. Glutamic acid and glycine alone constituted 78.49 % of the total amino acids. Zusammenfassung Stickstoffhaltige Bestandteile und freie Aminosäuren im Samenplasma von Büffeln (Bos Bubalis) in Relation zur Fruchtbarkeit während verschiedener Jahreszeiten Bei 8 Murrah‐Büffeln wurde während verschiedener Jahreszeiten im Spermaplasma der Gehalt an stickstoffhaltigen Komponenten und freien Aminosäuren analysiert. Befunde (mg / 100 ml): Totalstickstoff 649,10 ± 12,83; Proteinstickstoff 532 ± 11,56; Nichtproteinstickstoff 116,26 ± 2; Aminosäurenstickstoff 99,13 ± 4,4. Alle N‐Fraktionen variierten signifikant sowohl von Tier zu Tier als auch von Jahreszeit zu Jahreszeit. Alle N‐Fraktionen zeigten in den kühlen Perioden steigende Tendenz im Vergleich zu den warmen Perioden. 82 % des N entfielen auf das Eiweiß. Während der warmen Periode war die Konzeptionsrate am kleinsten. Der NPN und Aminosäuren‐N zeigten positive Korrelationen zur Konzeptionsrate. Mittels der zweidimensionalen Dünnschichtchromatographie wurden 10 verschiedene Aminosäuren (Asparaginsäure, Glutaminsäure, Serin, Alanin, Glycin, Lysin, Arginin, Histidin, Tyrosin, Tryptophan) identifiziert. Die Glutaminsäure dominierte gegenüber den anderen Aminosäuren (59,78 %), gefolgt von Glycin, Serin, Alanin und Asparaginsäure. Glutaminsäure und Glycin machten 78,49 % aller Aminosäuren aus. Résumé Proportion azotée et des acides aminés libres dans le plasma séminal chez des buffles (Bos bubalis) par rapport à la fertilité durant les différentes époques de l'année On a analysé à différentes périodes de l'année le taux des composés azotés et des acides aminés libres dans le plasma séminal chez 8 buffles Murrah. Résultats en mg/100 ml: azote total 649,10 ± 12,83; azote protéique 532 ± 11,56; azote non protéique 116,26 ± 2; azote d'acides aminés 99,13 ± 4,4. Toutes les fractions N ont varié significativement aussi d'un animal à l'autre que d'une période de l'année à l'autre. Toutes les fractions N montrèrent une tendance à la hausse durant les périodes froides par rapport a...

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The Free Amino Acids of Bovine Semen

Cited by 22 publications

References 11 publications

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Molecular approach of gossypol-induced reproductive toxicity in male rabbits. Changes in seminal plasma amino acids and fatty acids

Amino Acid Composition of Semen and the Secretions of the Male Reproductive Tract

Nitrogenous Constituents and Free Amino acid Content in the Seminal Plasma of Buffalo (Bos Bubalis) in Different Periods of the Year in Relation to Fertility

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