The frequencies of human neutrophil alloantigens in the Chinese Han population of Guangzhou

Xia, Wenjie; Bayat, Behnaz; Sachs, Ulrich J.; Chen, Yangkai; Shao, Yuang; Xu, Xiuzhang; Deng, Jing; Ding, Haoqiang; Fu, Yongshui; Ye, Xin; Santoso, Sentot

doi:10.1111/j.1537-2995.2010.02979.x

Cited by 42 publications

(88 citation statements)

References 47 publications

(40 reference statements)

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“…5). The HNA-3a encoding allele was demonstrated in Caucasians with a frequency of 0.79, in African Americans with 0.93 and in Chinese with 0.74, and the respective HNA-3b-encoding alleles with 0.21, 0.07 and 0.26 [82,83,84]. An additional SLC44A2*451C>T exchange (rs147820753) affecting the amino acid next to Arg152 and inducing a Leu151Phe substitution impairs the HNA-3a epitope in a way that the induction of neutrophil agglutination by some HNA-3a-specific alloantibodies is diminished or even abrogated [85].…”

Section: Hna-3mentioning

confidence: 99%

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Molecular Genetics of the Human Neutrophil Antigens

Flesch

Reil²

2018

Transfus Med Hemother

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Background and Objective: Antibodies to human neutrophil antigens (HNAs) have been implicated in transfusion-related acute lung injury and allo- and autoimmune neutropenia. To date, five HNA systems are assigned, and during the last decades enormous efforts have been undertaken to identify the underlying genes and to characterize the antigens. This review of the literature will provide the current genetic, molecular and functional information on HNAs. Recent Findings: New information on alleles and antigens has been added to nearly each of the five HNA systems. HNA-1d has been added as the antithetical epitope to HNA-1c that is located on the glycoprotein encoded by FCGR3B*02 but not by FCGR3B. FCGR3B*04 and *05 now are included as new alleles. A CD177*787A>T substitution was demonstrated as the main reason for the HNA-2-negative phenotype on neutrophils. The target glycoprotein of HNA-3 antibodies could be identified as choline transporter-like protein 2 (CTL2) encoded by SLC44A2. The conformation sensitive epitope discriminates between arginine and glutamine at position 152 resulting in HNA-3a and HNA-3b. An additional Leu151Phe substitution can impair HNA-3a antibody binding. Recently an alloantibody against HNA-4b which discriminates from HNA-4a by an Arg61His exchange of the glycoprotein encoded by the ITGAM gene was reported in neonatal alloimmune neutropenia. An update of the current HNA nomenclature based on the new findings was provided in 2016 by the ISBT Granulocyte Immunobiology Working Party nomenclature subcommittee. Conclusions: The molecular basis of each of the five HNA antigen systems has been decoded during the past decades. This enables reliable molecular typing strategies, antibody detection and specification as well as development of new assays based on recombinant antigens. However, research on HNA alleles, antigens, and antibodies is not finally terminated and also in the future will add new findings.

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Section: Hna-3mentioning

confidence: 99%

“…An ITGAM*230G>A substitution (rs1143679) is responsible for the translation into either 61Arg or the antithetical form with 61His (table 1) [9]. The HNA-4a allele frequencies in most populations have been determined with about 0.9-1.0 [84,90,92]. Only Taiwanese and Brazilians have lower frequencies with 0.65-0.92 [19,95].…”

Section: Hna-4mentioning

confidence: 99%

Molecular Genetics of the Human Neutrophil Antigens

Flesch

Reil²

2018

Transfus Med Hemother

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“…15 The target antigen HNA-3a is a high-frequency antigen with phenotype frequencies of 0.792 and 0.738 in white and Asian populations, respectively. 16 CTL2 is a multiple membrane-spanning glycoprotein, which is not only expressed on white blood cells but also on different tissues, including lung. CTL2 exists in 2 different isoforms, CTL2-P1 and CTL2-P2, which show variable distribution patterns among different cells.…”

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confidence: 99%

Anti–Human Neutrophil Antigen-3a Induced Transfusion-Related Acute Lung Injury in Mice by Direct Disturbance of Lung Endothelial Cells

Bayat

Tjahjono

Sydykov

et al. 2013

ATVB

Self Cite

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Objective— Antibodies against human neutrophil antigen-3a (HNA-3a) located on choline transporter-like protein 2 induce severe transfusion-related acute lung injury (TRALI). This study aims to identify the mechanism implicated in anti–HNA-3a-mediated TRALI. Approach and Results— Our analysis shows that anti–HNA-3a recognizes 2 choline transporter-like protein 2 isoforms (P1 and P2) on human microvascular endothelial cells from lung blood vessels but reacts only with the P1 isoform on neutrophils. Direct treatment of HNA-3a–positive endothelial cells with anti–HNA-3a, but not with anti–HNA-3b, leads to reactive oxygen species production, increased albumin influx, and decreased endothelial resistance associated with the formation of actin stress filaments and loosening of junctional vascular endothelium-cadherin. In a novel in vivo mouse model, TRALI was documented by significant increase in lung water content, albumin concentration, and neutrophil numbers in the bronchoalveolar lavage on injection of human anti–HNA-3a in lipopolysaccharides-treated, as well as nontreated mice. Interestingly, although neutrophil depletion alleviated severity of lung injury, it failed to prevent TRALI in this model. Infusion of anti–HNA-3a F(ab′) 2 fragments caused moderate TRALI. Finally, mice lacking nicotinamide adenine dinucleotide phosphate oxidase (NOX2 y/ - ) were protected from anti–HNA-3a-mediated TRALI. Conclusions— These data demonstrate the initiation of endothelial barrier dysfunction in vitro and in vivo by direct binding of anti–HNA-3a on endothelial cells. It seems, however, that the presence of neutrophils aggravates barrier dysfunction. This novel mechanism of TRALI primarily mediated by endothelial cell dysfunction via choline transporter-like protein 2 may help to define new treatment strategies to decrease TRALI-related mortality.

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“…Currently, 5 HNA systems (HNA-1 to HNA-5), have been characterized [5, 6]. In the HNA-1 system, 3 antigens, HNA-1a, HNA-1b and HNA-1c, are located on the neutrophil low-affinity Fc-γ-IIIb receptor (CD 16) glycoprotein [7].…”

Section: Introductionmentioning

confidence: 99%

Frequency of FCGR3B Alleles in Thai Blood Donors

et al. 2013

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BackgroundHuman neutrophil antigens (HNAs) are involved in autoimmune and alloimmune neutropenia and transfusion-related acute lung injury. The HNA-1 system is important in immunogenetics, and allele frequencies have been described in different populations. This study investigated the frequency of FCGR3B alleles encoding HNA-1a, HNA-1b, and HNA-1c among Thai blood donors and compared these frequencies with those previously reported for other populations.MethodsEight hundred DNA samples obtained from unrelated healthy blood donors at the National Blood Centre, Thai Red Cross Society, Bangkok, and the Blood Bank, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, were included. Samples were simultaneously typed for each FCGR3B allele using an in-house polymerase chain reaction with sequence-specific primer (PCR-SSP) technique.ResultsThe frequencies of FCGR3B*1, FCGR3B*2, and FCGR3B*3 alleles in central Thai blood donors were 0.548, 0.452, and 0.004, respectively; only FCGR3B*1 and FCGR3B*2 alleles were found in northern Thai blood donors (0.68 and 0.32, respectively). Compared with other Asian populations, central Thais had higher frequencies of the FCGR3B*2 allele (P<0.001), while the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in northern Thais were similar to those previously reported in Taiwanese and Japanese populations. In contrast, the frequencies of the FCGR3B*1 and FCGR3B*2 alleles in the northern Thai population were statistically different from those observed in central Thai, Korean, German, and Turkish populations.ConclusionsFCGR3B allele frequencies were significantly different between central and northern Thai blood donors. Our in-house PCR-SSP method is a simple, cost-effective, and convenient method for FCGR3B allele detection.

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The frequencies of human neutrophil alloantigens in the Chinese Han population of Guangzhou

Cited by 42 publications

References 47 publications

Molecular Genetics of the Human Neutrophil Antigens

Molecular Genetics of the Human Neutrophil Antigens

Anti–Human Neutrophil Antigen-3a Induced Transfusion-Related Acute Lung Injury in Mice by Direct Disturbance of Lung Endothelial Cells

Frequency of FCGR3B Alleles in Thai Blood Donors

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