The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis

Saito, Ryoichi; Nonaka, Shotaro; Fujinami, Yuji; Matsuoka, Satoshi; Nakajima, Shinichi; Nishiyama, Hiroyuki; Okamura, Noboru

doi:10.1016/j.jiac.2013.06.003

Cited by 16 publications

(19 citation statements)

References 12 publications

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“…Discks of beta-lactam antibiotics have been used in natural resistance to each type or kind of bacteria. The method, Etest was also used as described by Saito et al (2013).…”

Section: Strains Susceptibilitymentioning

confidence: 99%

Activity of beta-lactam antibiotics and production of beta-lactamases in bacteria isolated from wound infections in Brazzaville, Congo

Moyen¹,

Ahombo²,

Nguimbi³

et al. 2014

Afr. J. Microbiol. Res.

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To determine the mechanism of bacterial beta-lactam resistance, 165 bacteria isolated from wounds of hospitalized patients composing of: 42 Staphylococcus aureus, 37 Pseudomonas aeruginosa, 23 Escherichia coli, 22 Proteus, 12 Klebsiella, 10 coagulase-negative staphylococci (CNS), eight Enterobacter, six Citrobacter, five Providencia were tested for their sensitivity to beta-lactams and their production of beta-lactamases. The antibiotic susceptibility was considered by the method of the standard diffusion on agar Mueller Hinton. The rate of production of ß-lactamase in all bacteria was determined using the Strips of nitrocefin. The percentages of resistance to beta-lactams obtained were as follows: Staphylococcus aureus (77.90%), Pseudomonas aeruginosa (44.14%), E. coli (73.8%), Proteus (57.4%), Klebsiella (63.6%), CNS (57.15%), Enterobacter (56.3%), Citrobacter (83.3%), Providencia (67.5%). The rate of beta-lactamases were as follows: S. aureus (7.34%), P. aeruginosa (89.19%), E. coli (95.65%), Proteus (86.36%), Klebsiella (91.67%), CNS (90%), Enterobacter (87.5%), Citrobacter (66.67), Providencia (100%). The studied bacteria produce beta-lactamases which is the primary mechanism of resistance to beta-lactam antibiotics in the majority of the bacteria. Beta-lactamases rates vary from one genus to another. It is extended spectrum beta-lactamase-producing strain.

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“…Discks of beta-lactam antibiotics have been used in natural resistance to each type or kind of bacteria. The method, Etest was also used as described by Saito et al (2013).…”

Section: Strains Susceptibilitymentioning

confidence: 99%

Activity of beta-lactam antibiotics and production of beta-lactamases in bacteria isolated from wound infections in Brazzaville, Congo

Moyen¹,

Ahombo²,

Nguimbi³

et al. 2014

Afr. J. Microbiol. Res.

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“…However, due to the development of antibiotic resistance of these otopathogens (4), acute OM can still progress to chronic or recurrent infections, and complications with significant morbidity even occur (5). It has been reported that more than 90% of M. catarrhalis isolates are beta‐lactamase positive (6). The extensive production of beta‐lactamase enzymes makes M. catarrhalis resistant to antibiotic treatment, and no vaccine is available for this otopathogen, suggesting infections with M. catarrhalis may become more prevalent (6,7).…”

Section: Introductionmentioning

confidence: 99%

Photoinactivation of Moraxella catarrhalis Using 405‐nm Blue Light: Implications for the Treatment of Otitis Media

Liu

Chang

Ferrer‐Espada

et al. 2020

Photochem & Photobiology

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Moraxella catarrhalis is one of the major otopathogens of otitis media (OM) in childhood. M. catarrhalis tends to form biofilm, which contributes to the chronicity and recurrence of infections, as well as resistance to antibiotic treatment. In this study, we aimed to investigate the effectiveness of antimicrobial blue light (aBL; 405 nm), an innovative nonpharmacological approach, for the inactivation of M. catarrhalis OM. M. catarrhalis either in planktonic suspensions or 24‐h old biofilms were exposed to aBL at the irradiance of 60 mW cm−2. Under an aBL exposure of 216 J cm−2, a >4‐log10 colony‐forming units (CFU) reduction in planktonic suspensions and a >3‐log10 CFU reduction in biofilms were observed. Both transmission electron microscopy and scanning electron microscopy revealed aBL‐induced morphological damage in M. catarrhalis. Ultraperformance liquid chromatography results indicated that protoporphyrin IX and coproporphyrin were the two most abundant species of endogenous photosensitizing porphyrins. No statistically significant reduction in the viability of HaCaT cells was observed after an aBL exposure of up to 216 J cm−2. Collectively, our results suggest that aBL is potentially an effective and safe alternative therapy for OM caused by M. catarrhalis. Further in vivo studies are warranted before this optical approach can be moved to the clinics.

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“… 83 Resistance to antibiotics is of concern as well, with studies reporting that over 95% of clinical M. catarrhalis isolates are resistant to the β-lactamase family of antibiotics. 84 , 85 Polymicrobial biofilms composed of M. catarrhalis and S. pneumoniae appear to contribute to antibiotic resistance in patients with otitis media. 86 …”

Section: Introductionmentioning

confidence: 99%

Bacterial infections in patients with primary ciliary dyskinesia: Comparison with cystic fibrosis

2017

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Primary ciliary dyskinesia (PCD) is an autosomal recessive disorder associated with severely impaired mucociliary clearance caused by defects in ciliary structure and function. Although recurrent bacterial infection of the respiratory tract is one of the major clinical features of this disease, PCD airway microbiology is understudied. Despite the differences in pathophysiology, assumptions about respiratory tract infections in patients with PCD are often extrapolated from cystic fibrosis (CF) airway microbiology. This review aims to summarize the current understanding of bacterial infections in patients with PCD, including infections with Pseudomonas aeruginosa, Staphylococcus aureus, and Moraxella catarrhalis, as it relates to bacterial infections in patients with CF. Further, we will discuss current and potential future treatment strategies aimed at improving the care of patients with PCD suffering from recurring bacterial infections.

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The frequency of BRO β-lactamase and its relationship to antimicrobial susceptibility and serum resistance in Moraxella catarrhalis

Cited by 16 publications

References 12 publications

Activity of beta-lactam antibiotics and production of beta-lactamases in bacteria isolated from wound infections in Brazzaville, Congo

Activity of beta-lactam antibiotics and production of beta-lactamases in bacteria isolated from wound infections in Brazzaville, Congo

Photoinactivation of Moraxella catarrhalis Using 405‐nm Blue Light: Implications for the Treatment of Otitis Media

Bacterial infections in patients with primary ciliary dyskinesia: Comparison with cystic fibrosis

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