The frequency of Klebsiella pneumonia encoding genes for CTX-M, TEM-1 and SHV-1 extended-spectrum beta lactamases enzymes isolated from urinary tract infection

Dehshiri, Masomeh; Khoramrooz, Seyed Sajjad; Zoladl, Mohammad; Khosravani, Seyed Abdolmajid; Parhizgari, Najmeh; Motazedian, Mohammad Hossein; Jahedi, Soheyla; Sharifi, Asghar

doi:10.1186/s12941-018-0256-y

Cited by 26 publications

(14 citation statements)

References 13 publications

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“…Klebsiella and Enterococcus were relatively increased in the diabetes group. Klebsiella is a common conditional pathogenic bacterium that causing respiratory and urinary tract infections when host immunity is impaired [22]. Enterococcus is a nosocomial pathogen which can cause UTI and endocarditis [23].…”

Section: Discussionmentioning

confidence: 99%

Relationship between alterations of urinary microbiota and cultured negative lower urinary tract symptoms in female type 2 diabetes patients

et al. 2019

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Background Lower urinary tract symptoms (LUTS) is the most common complication of diabetes. However, the underlying pathogenesis of cultured negative LUTS (cn-LUTS) in diabetic patients has not been well understood. Numerous evidence indicates that urinary dysbiosis is related to urologic disorders. We aim to study alterations of the urinary microbiota of cn-LUTS in type 2 diabetes (T2D) patients. Methods Female T2D patients and controls were recruited and requested to finish the American Urological Association Symptom Index. Mid-stream urine was collected for culturing and extracting DNA. Microbial diversity and composition were analyzed by targeting to 16S rDNA. Linear discriminant analysis effect size (LEfSe) was carried out to identify significantly different bacteria. Results 32 female T2D patients and 26 controls were enrolled. No significant differences in alpha diversity were observed between patients and controls. However, statistically decreased richness (ACE index and Chao 1 index, 85.52(13.75, 204.84) vs. 129.82(63.89, 280.30) and 83.86(11.00, 210.77) vs. 125.19(62.00, 251.77), P = 0.005; Observed Species, 76(10, 175) vs. 98(54, 234), P = 0.011) and decreased species diversity (Shannon index, 1.37(0.04, 3.48) vs. 2.09(0.98, 3.43), P = 0.033; Simpson index, 0.46 (0.06, 0.99) vs. 0.23(0.07, 0.64), P = 0.029) were shown in moderate-to-severe LUTS group and high Hemoglobin A1c group, respectively. A significant difference of beta diversity was found between T2D patients and controls and T2D patients with different severity of cn-LUTS as well as the different level of Hemoglobin A1c. LEfSe revealed that 10 genera (e.g., Escherichia-Shigella and Klebsiella ) were increased and 7 genera were decreasing in T2D patients, 3 genera (e.g., Escherichia-Shigella and Campylobacter ) were increased and 16 genera (e.g., Prevotella ) were reduced in moderate-to-severe LUTS group, 2 genera ( Escherichia-Shigella and Lactobacillus ) were over-represented and 10 genera (e.g., Prevotella ) were under-represented in high Hemoglobin A1c group. Finally, Hemoglobin A1c was found positively correlated with the total score of the American Urological Association Symptom Index ( r = 0.509, P = 0.003). Conclusions Urinary dysbiosis may be related to cn-LUTS in female T2D patients. A better understanding of urinary microbiota in the development and progression of cn-LUTS in female T2D patients was necessary. The severity of cn-LUTS was correlated to hyperglycemia and chronic hyperglycemia might induce or promote cn-LUTS by influencing urinary microbiota. ...

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Section: Discussionmentioning

confidence: 99%

Relationship between alterations of urinary microbiota and cultured negative lower urinary tract symptoms in female type 2 diabetes patients

et al. 2019

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“…The distribution of blaSHV depends on the rate of ESBL infection caused by K.pneumoniae . A study by Dehshiri et al,38 on the occurrence of ESBL-producing K.pneumoniae in patients with urinary tract infection concluded that the prevalence of blaSHV was very high (85%), compared with that of blaCTX-M and blaTEM at 30% and 16%. In the present investigation, the prevalence of blaSHV was greater in K.pneumoniae (56.7%) than E.coli (17.7%).…”

Section: Discussionmentioning

confidence: 99%

Risk factors and molecular features of extended-spectrum beta-lactamase producing bacteria at southwest of Saudi Arabia

Al-Garni

Ghonaim

Ahmed

et al. 2018

SMJ

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Objectives:To explore the risk factors, the prevalence rate, and gene types of extended-spectrum beta-lactamase (ESBL)-producing bacteria as the causative agents of infection at King Abdulaziz Specialist Hospital (KAASH), Taif, Kingdom of Saudi Arabia.Methods:This was a retrospective study conducted during the period between February 2017 and January 2018. All samples obtained from the KAASH were analyzed. The MicroScan Walkaway System, bacteriological examination and double disk synergy tests were used to detect ESBL-producing bacteria. To identify ESBL genes, the polymerase chain reaction (PCR) technique was used.Results:The ESBL phenotype was detected in 351 of 1151 isolates (30.5%); Escherichia coli (E. coli) (62.7%) and Klebsiella pneumoniae (K. pneumoniae) (23.6%) were the most prevalent. The highest proportion of ESBL specimens was found in urine (62%.5), and these organisms were mainly isolated from the female medical ward (20.2%). Based on the statistical analysis, lung diseases, renal diseases, diabetes and heart diseases contributed to the spread of ESBL infections. Amikacin, imipenem, meropenem and tigecycline were found to be effective in overcoming ESBL infections; however, these antibiotics may be inappropriate for new strains of K.pneumoniae. The distribution of the blaCTX-M gene was high (87%), compared with blaTEM (74.9%) and blaSHV (29.4%).Conclusion:These data provide new epidemiological information about the prevalence of ESBL-producing organisms among patients in KAASH, Taif, Saudi Arabia. In addition, this study identified the clonal nature of isolated E.coli and K.pneumoniae.

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“…In Iran, studies have shown that in Klebsiella spp., resistance to extended spectrum beta lactams is mainly mediated via the production of CTX-M variants (CTX-M-15, CTX-M-3, CTX-M-8, CTX-M-1, and CTX-M-2) (Feizabadi et al, 2010; Peerayeh et al, 2014; Bialvaei et al, 2016; Akya et al, 2018; Dehshiri et al, 2018) and to a lesser degree via SHV (SHV-12, SHV-11, SHV-5) (Feizabadi et al, 2010; Shakib et al, 2018), TEM genes (Gholipour et al, 2014; Maleki et al, 2018) and others (VEB, PER, and GES) (Sedighi et al, 2017). pAmpC beta lactamase genes were reported by two studies in clinical isolates of Klebsiella pneumoniae (Mansouri et al, 2012; Kiaei et al, 2018).…”

Section: Distribution Of Multi-drug Resistant Organisms In Humansmentioning

confidence: 99%

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

et al. 2019

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In the last decade, extended-spectrum cephalosporin and carbapenem resistant Gram-negative bacilli (GNB) have been extensively reported in the literature as being disseminated in humans but also in animals and the environment. These resistant organisms often cause treatment challenges due to their wide spectrum of antibiotic resistance. With the emergence of colistin resistance in animals and its subsequent detection in humans, the situation has worsened. Several studies reported the transmission of resistant organisms from animals to humans. Studies from the middle east highlight the spread of resistant organisms in hospitals and to a lesser extent in livestock and the environment. In view of the recent socio-economical conflicts that these countries are facing in addition to the constant population mobilization; we attempt in this review to highlight the gaps of the prevalence of resistance, antibiotic consumption reports, infection control measures and other risk factors contributing in particular to the spread of resistance in these countries. In hospitals, carbapenemases producers appear to be dominant. In contrast, extended spectrum beta lactamases (ESBL) and colistin resistance are becoming a serious problem in animals. This is mainly due to the continuous use of colistin in veterinary medicine even though it is now abandoned in the human sphere. In the environment, despite the small number of reports, ESBL and carbapenemases producers were both detected. This highlights the importance of the latter as a bridge between humans and animals in the transmission chain. In this review, we note that in the majority of the Middle Eastern area, little is known about the level of antibiotic consumption especially in the community and animal farms. Furthermore, some countries are currently facing issues with immigrants, poverty and poor living conditions which has been imposed by the civil war crisis. This all greatly facilitates the dissemination of resistance in all environments. In the one health concept, this work re-emphasizes the need to have global intervention measures to avoid dissemination of antibiotic resistance in humans, animals and the environment in Middle Eastern countries.

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The frequency of Klebsiella pneumonia encoding genes for CTX-M, TEM-1 and SHV-1 extended-spectrum beta lactamases enzymes isolated from urinary tract infection

Cited by 26 publications

References 13 publications

Relationship between alterations of urinary microbiota and cultured negative lower urinary tract symptoms in female type 2 diabetes patients

Relationship between alterations of urinary microbiota and cultured negative lower urinary tract symptoms in female type 2 diabetes patients

Risk factors and molecular features of extended-spectrum beta-lactamase producing bacteria at southwest of Saudi Arabia

Understanding the Epidemiology of Multi-Drug Resistant Gram-Negative Bacilli in the Middle East Using a One Health Approach

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