The frequency of toll-like receptor 4 gene polymorphism in ankylosing spondylitis and its relationship between disease activity

Abstract

Objective. We aimed to evaluate the frequency of toll-like receptor 4 (TLR4) gene polymorphism and its relationship between disease activity in patients with ankylosing spondylitis (AS). Methods. Forty-one AS patients (25 male/16 female) fullfilling the 1984 Modified New York Criteria and 41 healthy controls (25 male/16 female) were included in this study. Disease activity of the AS patients was assesed by Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). The TLR4 gene polymorphism of AS patients and healthy controls were analyzed by Real-Time Polymerase Chain Reaction (PCR) System. Results. Three (7.3%) patients with AS had TLR4 gene polymorphism compared with healthy controls (0/41; 0%). Two of these patients had heterozygous mutation and one had homozygous mutation. Significant correlation was not found between TLR4 gene polymorphism and BASDAI score (p > 0.05). Conclusions. In our study, TLR4 gene polymorphism was higher in patients with AS compared with control group. But, this polymorphism was not associated with disease activity, erythrocyte sedimentation rate and C-reactive protein levels.

Associations of toll‐like receptor 4 and 2 gene polymorphisms with susceptibility to ankylosing spondylitis: A meta‐analysis

“…The remaining 18 articles were full‐text reviewed, and 5 articles were excluded. Finally, 13 studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Guo, 2019; Machado et al., 2016; Na et al., 2008; Oliveira‐Tore et al., 2019; van der Paardt et al., 2005; Qin et al., 2009; Sargın et al., 2017; Snelgrove et al., 2007; Sode et al., 2018; Wu et al., 2012) with sufficient data went into qualitative synthesis. The detailed flowchart of literature selection is displayed in Figure 1.…”

“…The studies were published between 2005 and 2019. Two studies (Guo, 2019; Qin et al., 2009) were published in Chinese, and the rest (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; Oliveira‐Tore et al., 2019; van der Paardt et al., 2005; Sargın et al., 2017; Snelgrove et al., 2007; Sode et al., 2018; Wu et al., 2012) were in English. Seven studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; van der Paardt et al., 2005; Sargın et al., 2017; Snelgrove et al., 2007; Sode et al., 2018) were conducted in European population, four studies (Guo, 2019; Na et al., 2008; Qin et al., 2009; Wu et al., 2012) were carried out in Asian population, and two studies (Machado et al., 2016; Oliveira‐Tore et al., 2019) were performed in mixed ethnicities population.…”

“…Two studies (Guo, 2019; Qin et al., 2009) were published in Chinese, and the rest (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; Oliveira‐Tore et al., 2019; van der Paardt et al., 2005; Sargın et al., 2017; Snelgrove et al., 2007; Sode et al., 2018; Wu et al., 2012) were in English. Seven studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; van der Paardt et al., 2005; Sargın et al., 2017; Snelgrove et al., 2007; Sode et al., 2018) were conducted in European population, four studies (Guo, 2019; Na et al., 2008; Qin et al., 2009; Wu et al., 2012) were carried out in Asian population, and two studies (Machado et al., 2016; Oliveira‐Tore et al., 2019) were performed in mixed ethnicities population. Of the included studies, nine studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; van der Paardt et al., 2005; Qin et al., 2009; Sargın et al., 2017; Snelgrove et al., 2007) were on TLR4‐rs4986790 , seven studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; Qin et al., 2009; Snelgrove et al., 2007) on TLR4‐rs4986791 , two studies (Guo, 2019; Wu et al., 2012) on TLR9‐rs55704465 , and two studies (Oliveira‐Tore et al., 2019; Sode et al., 2018) on TLR9‐rs187084 .…”

“…Seven studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; van der Paardt et al., 2005; Sargın et al., 2017; Snelgrove et al., 2007; Sode et al., 2018) were conducted in European population, four studies (Guo, 2019; Na et al., 2008; Qin et al., 2009; Wu et al., 2012) were carried out in Asian population, and two studies (Machado et al., 2016; Oliveira‐Tore et al., 2019) were performed in mixed ethnicities population. Of the included studies, nine studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; van der Paardt et al., 2005; Qin et al., 2009; Sargın et al., 2017; Snelgrove et al., 2007) were on TLR4‐rs4986790 , seven studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; Qin et al., 2009; Snelgrove et al., 2007) on TLR4‐rs4986791 , two studies (Guo, 2019; Wu et al., 2012) on TLR9‐rs55704465 , and two studies (Oliveira‐Tore et al., 2019; Sode et al., 2018) on TLR9‐rs187084 . The results of HWE were not available in three studies (Na et al., 2008; Qin et al., 2009; Sargın et al., 2017) because there were no mutant genotypes in the control participants.…”

“…Of the included studies, nine studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; van der Paardt et al., 2005; Qin et al., 2009; Sargın et al., 2017; Snelgrove et al., 2007) were on TLR4‐rs4986790 , seven studies (Adam et al., 2006; Burton et al., 2007; Gergely et al., 2006; Machado et al., 2016; Na et al., 2008; Qin et al., 2009; Snelgrove et al., 2007) on TLR4‐rs4986791 , two studies (Guo, 2019; Wu et al., 2012) on TLR9‐rs55704465 , and two studies (Oliveira‐Tore et al., 2019; Sode et al., 2018) on TLR9‐rs187084 . The results of HWE were not available in three studies (Na et al., 2008; Qin et al., 2009; Sargın et al., 2017) because there were no mutant genotypes in the control participants. The rest studies were in HWE, with the exception of the study by Wu et al.…”