1999
DOI: 10.1016/s0006-3495(99)77393-5
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The Frequency Response of Smooth Muscle Stiffness during Ca2+-Activated Contraction

Abstract: To investigate the mechanism of smooth muscle contraction, the frequency response of the muscle stiffness of single beta-escin permeabilized smooth muscle cells in the relaxed state was studied. Also, the response was continuously monitored for 3 min from the beginning of the exchange of relaxing solution to activating solution, and then at 5-min intervals for up to 20 min. The frequency response (30 Hz bandwidth, 0.33 Hz (or 0.2 Hz) resolution) was calculated from the Fourier-transformed force and length samp… Show more

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Cited by 27 publications
(33 citation statements)
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“…For mechanical measurements, single SMC were attached to a force transducer (series 400A, Aurora Scientific Inc., Aurora, Ontario, Canada) with glue (Polycel, Maklanberg-Duncan, Oklahoma City, OK) as described previously (12). SMCs were grown on Matrigel TM -coated coverslips (13) in low serum culture dishes for 24 -48 h after transfection and were then transferred to a chamber on the movable stage of a Nikon inverted florescent microscope.…”
Section: Methodsmentioning
confidence: 99%
“…For mechanical measurements, single SMC were attached to a force transducer (series 400A, Aurora Scientific Inc., Aurora, Ontario, Canada) with glue (Polycel, Maklanberg-Duncan, Oklahoma City, OK) as described previously (12). SMCs were grown on Matrigel TM -coated coverslips (13) in low serum culture dishes for 24 -48 h after transfection and were then transferred to a chamber on the movable stage of a Nikon inverted florescent microscope.…”
Section: Methodsmentioning
confidence: 99%
“…Before experiments, the tip of the cantilevers was thinly coated with a polyurethane adhesive [Shue and Brozovich 1999;Smith et al 2000;Nagayama et al 2006] by using another cantilever having a mass of the adhesive at its tip. The suspension dish containing the floating cells was then mounted on the stage of the microscope.…”
Section: Tensile Testmentioning
confidence: 99%
“…Originally, the cell was held by aspirating into the two micropipettes. In this study, the cell was held by gently pressing onto its surface two pipettes that had been coated with a urethane resin adhesive (Sista M5250, Henkel) (11), (12) . We have already confirmed that the tensile properties obtained from SMCs using the two methodologies were almost similar (13) .…”
Section: Cell Tensile Testermentioning
confidence: 99%