The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against <i>Phytophthora infestans</i> in <i>Nicotiana benthamiana</i>

Shibata, Yusuke; Ojika, Makoto; Sugiyama, Akifumi; Yazaki, Kazufumi; Jones, David A.; Kawakita, Kazuhito; Takemoto, Daigo

doi:10.1105/tpc.15.00721

Cited by 71 publications

(91 citation statements)

References 86 publications

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“…3G). Previous studies of AtABCG36/PEN3 and NbABCG1/2 showed that these transporters are preferentially localized at the pathogen penetration sites (5,11). However, we did not observe such preferential accumulation of AtABCG34 at the penetration site.…”

Section: Discussioncontrasting

confidence: 99%

“…First, we counted the pathogen penetration sites by callose staining in the wild-type plants, atabcg34 mutants, and complementation lines. Callose is deposited at the penetration site in response to A. brassicicola treatment (28), and a high number of callose depositions indicates a failure to overcome the pathogen at the preinvasion stage (5,29). atabcg34 mutants exhibited a significantly higher number of penetration sites than did the wild-type and complementation lines (Fig.…”

Section: Resultsmentioning

confidence: 96%

“…Other ABC transporters important for resistance at the preinvasion stage include NtPDR1 and NpPDR1, which transport sclareol (2, 3), an important factor for preinvasion resistance (5). Recently, NbABCG1 and NbABCG2 were reported to be important for both pre-and postinvasion resistance in N. benthamiana (5).…”

Section: Discussionmentioning

confidence: 99%

“…Two functionally redundant Nicotiana benthamiana full-size ABCG transporters, NbABCG1 and NbABCG2, are essential for resistance to Phytophthora infestans. Mutants with defects in these two ABCG transporters export much less capsidiol than do the corresponding wild-type plants, indicating that these transporters transport capsidiol (5). NtABCG5/ NtPDR5 is induced by insect herbivory, and the absence of this transporter decreases resistance to insects, indicating that it is involved in the transport of a hitherto unidentified insecticidal compound (6).…”

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confidence: 99%

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Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin

Khare

Choi

Huh

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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Plant pathogens cause huge yield losses. Plant defense often depends on toxic secondary metabolites that inhibit pathogen growth. Because most secondary metabolites are also toxic to the plant, specific transporters are needed to deliver them to the pathogens. To identify the transporters that function in plant defense, we screened Arabidopsis thaliana mutants of full-size ABCG transporters for hypersensitivity to sclareol, an antifungal compound. We found that atabcg34 mutants were hypersensitive to sclareol and to the necrotrophic fungi Alternaria brassicicola and Botrytis cinerea. AtABCG34 expression was induced by A. brassicicola inoculation as well as by methyl-jasmonate, a defense-related phytohormone, and AtABCG34 was polarly localized at the external face of the plasma membrane of epidermal cells of leaves and roots. atabcg34 mutants secreted less camalexin, a major phytoalexin in A. thaliana, whereas plants overexpressing AtABCG34 secreted more camalexin to the leaf surface and were more resistant to the pathogen. When treated with exogenous camalexin, atabcg34 mutants exhibited hypersensitivity, whereas BY2 cells expressing AtABCG34 exhibited improved resistance. Analyses of natural Arabidopsis accessions revealed that AtABCG34 contributes to the disease resistance in naturally occurring genetic variants, albeit to a small extent. Together, our data suggest that AtABCG34 mediates camalexin secretion to the leaf surface and thereby prevents A. brassicicola infection.AtABCG34 | ABC transporters | camalexin | A. brassicicola | B. cinerea P lants are exposed to a multitude of pathogens, but they usually resist infection by using their unique defense systems and compounds, including secondary metabolites produced either constitutively (phytoanticipins) or in response to pathogen attack (phytoalexins). Plants produce tens of thousands of secondary metabolites, which are classified as phenolics, terpenoids, alkaloids, glucosinolates, cyanogenic glucosides, and betanins. Secondary metabolites are secreted from the infected cells and their surrounding cells after pathogen attack or are released, hydrolyzed, and become toxic when the cells are destroyed by pathogens (1), thus inhibiting pathogen growth on the plant. Many secondary metabolites are dangerous to the plants because of their toxicity to cellular metabolism. To avoid self-toxicity, plants either secrete these compounds to the leaf surface or sequester them in the vacuole, a compartment with low metabolic activity. In both cases, specific transporters are required either to deliver the secondary metabolites to the site where the pathogens are located or to store them as weapons against pathogen attack.Several full-size ABCG transporters are involved in the transport of secondary metabolites. Nicotiana plumbaginifolia PDR1/ABC1 and Nicotiana tabacum PDR1 are induced by jasmonate, a defense-related hormone highly expressed in the leaf epidermal cells and trichomes, and transport sclareol, a diterpene alcohol secreted by Nicotiana species in response to p...

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Section: Discussioncontrasting

confidence: 99%

Section: Resultsmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin

Khare

Choi

Huh

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…and Ran et al . (Earley et al, 2006; Li et al, 2014), and a reporter fusion was also constructed for the A. thaliana plasma membrane (PM) intrinsic protein 2a (accession number: X75883) which was previously characterized as a marker for membrane associations (Shibata et al, 2016). The open reading frame (ORF) of NaLRRK1 and PM were firstly amplified with Phusion Green High-Fidelity DNA polymerase (Thermo) by primer pairs listed in the Supplementary file 1D; an additional sequence (CACC) was then introduced into the forward primers to facilitate directional cloning into the pENTR/D-TOPO vector (Thermo).…”

Section: Methodsmentioning

confidence: 99%

Evolution of herbivore-induced early defense signaling was shaped by genome-wide duplications in Nicotiana

Zhou

Brockmöller

Ling

et al. 2016

eLife

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Herbivore-induced defenses are widespread, rapidly evolving and relevant for plant fitness. Such induced defenses are often mediated by early defense signaling (EDS) rapidly activated by the perception of herbivore associated elicitors (HAE) that includes transient accumulations of jasmonic acid (JA). Analyzing 60 HAE-induced leaf transcriptomes from closely-related Nicotiana species revealed a key gene co-expression network (M4 module) which is co-activated with the HAE-induced JA accumulations but is elicited independently of JA, as revealed in plants silenced in JA signaling. Functional annotations of the M4 module were consistent with roles in EDS and a newly identified hub gene of the M4 module (NaLRRK1) mediates a negative feedback loop with JA signaling. Phylogenomic analysis revealed preferential gene retention after genome-wide duplications shaped the evolution of HAE-induced EDS in Nicotiana. These results highlight the importance of genome-wide duplications in the evolution of adaptive traits in plants.DOI: http://dx.doi.org/10.7554/eLife.19531.001

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Endophytic Bacteria Enterobacter cloacae PN7 Promotes Biosynthesis and Accumulation of Saponins in Panax notoginseng

Lin,

Wang,

Xie

et al. 2024

Curr Microbiol

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The Full-Size ABCG Transporters Nb-ABCG1 and Nb-ABCG2 Function in Pre- and Postinvasion Defense against Phytophthora infestans in Nicotiana benthamiana

Cited by 71 publications

References 86 publications

Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin

Arabidopsis ABCG34 contributes to defense against necrotrophic pathogens by mediating the secretion of camalexin

Evolution of herbivore-induced early defense signaling was shaped by genome-wide duplications in Nicotiana

Endophytic Bacteria Enterobacter cloacae PN7 Promotes Biosynthesis and Accumulation of Saponins in Panax notoginseng

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