The function of cellular transcobalamin II in cultured human cells

Hall, Charles A.; Colligan, Pamela D.

doi:10.1016/0014-4827(89)90426-6

Cited by 9 publications

(5 citation statements)

References 15 publications

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“…Cultured fibroblasts were derived from skin biopsies taken under local anesthesia. Fibroblast culture and TC-I1 function studies were performed as previously described (22). In brief, serum samples were labeled to excess with [57Co]CN-Cbl, and free label was removed with albumin-coated charcoal to measure binding capacity.…”

Section: Methodsmentioning

confidence: 99%

Inherited Selective Intestinal Cobalamin Malabsorption and Cobalamin Deficiency in Dogs

et al. 1991

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ABSTRACT. Inherited selective intestinal malabsorption of cobalamin (Cbl) was observed in a family of giant schnauzer dogs. Family studies and breeding experiments demonstrated simple autosomal recessive inheritance of this disease. Affected puppies exhibited chronic inappetence and failure to thrive beginning between 6 and 12 wk of age. Neutropenia with hypersegmentation, anemia with anisocytosis and poikilocytosis, and megaloblastic changes of the bone marrow were present. Serum Cbl concentrations were low, and methylmalonic aciduria and homocysteinemia were present. Parenteral, but not oral, cyanocobalamin administration rapidly eliminated all signs of Cbl deficiency except for low serum Cbl concentrations. Cbl malabsorption in affected dogs was documented by oral administration of [57Co]cyanocobalamin with or without simultaneous oral administration of intrinsic factor or normal dog gastric juice. Quantitation and function studies of intrinsic factor and transcobalamin-I1 from affected dogs revealed no abnormality. Other gastrointestinal functions and ileal morphology were normal, indicating a selective defect of Cbl absorption at the level of the ileal enterocyte. Immunoelectron microscopy of ileal biopsies showed that the receptor for intrinsic factor-Cbl complex was absent from the apical brush border microvillus pits of affected dogs. This canine disorder resembles inherited selective intestinal Cbl malabsorption (Imerslund-Grasbeck syndrome)

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Section: Methodsmentioning

confidence: 99%

Inherited Selective Intestinal Cobalamin Malabsorption and Cobalamin Deficiency in Dogs

et al. 1991

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“…174 Indeed, it was shown that the cell membrane receptor TCII is up-regulated in various malignant cells during thymidine synthesis. 175 This strong demand for B 12 makes antivitamins B 12 highly attractive for the design of therapeutic drugs. 15,50 Antivitamins are compounds that counteract the essential effects of vitamins, e.g.…”

Section: Antivitamins B 12mentioning

confidence: 99%

“…Synthesis of deoxythymidine monophosphate (dTMP) from deoxyuridine monophosphate (dUMP).For this reason, some rapidly proliferating cell lines and sites of bacterial infection have an elevated demand for B 12 174. Indeed, it was shown that the cell membrane receptor TCII is upregulated in various malignant cells during thymidine synthesis 175. This strong demand for B 12 makes antivitamins B 12 highly attractive for the design of therapeutic drugs 15,50.…”

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confidence: 99%

Recent trends in the development of vitamin B₁₂derivatives for medicinal applications

Zelder

2015

Chem. Commun.

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This Feature Article highlights recent developments in the field of vitamin B12 derivatives for medicinal applications. The following topics are emphasized: (1) the development of aquacorrinoids for cyanide detection and detoxification, (2) the use of vitamin B12 conjugates and (3) antivitamins B12 for therapy and diagnosis, and (4) the design of corrinoids as activators of soluble guanylyl cyclase (sGC).

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“…The cellular actions of vitamin B 12 are dependent on vitamin B 12 uptake into the cell either by internalization of free cobalamin or, more important, on receptor mediated uptake of the transcobalamin II (TCII)/vitamin B 12 complex into the cells via the TCII-receptor [18]. It has been shown, that cultured cells produce endogenous TCII and the amount of internalized cobalamin correlates directly to the capacity of the cells to produce TCII [19]. Our data suggests that in the in vitro model of HSE cellular expression of TCII and TCII-receptor occurs due to the lack of vitamin B 12 in the tissue culture medium.…”

Section: Discussionmentioning

confidence: 99%

Vitamin B12 Activates the Wnt-Pathway in Human Hair Follicle Cells by Induction of <i>beta</i>-Catenin and Inhibition of Glycogensynthase Kinase-3 Transcription

Krugluger¹,

Stiefsohn²,

Laciak³

et al. 2011

JCDSA

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Background and Objectives: Micrograft transplantation is accompanied by a transient induction of telogen in transplanted hair follicles (HF), which might be avoided by supporting the metabolic pathways of the micrograft during the ex vivo period. Vitamin B 12 (cobalamin) has been suggested to influence HF growth and cycling in humans, but the mechanisms are unclear. Method: HFs were obtained from patients undergoing routine micrograft transplantation and were cultured for 5 days in Dulbecco's modified Eagles Medium, supplemented with different amounts of vitamin B 12. Hair shaft elongation (HSE) of the isolated HFs as well as quantitative changes of mRNA for -catenin, glykogensynthase kinase-3 (GSK-3) and TCF/Lef-1 in HF cells were determined. Results: In vitro HSE demonstrated a dose dependent induction of HSE after stimulation with 2.5 g/ml and 25 g/ml vitamin B 12 (6.2  2.1% and 15.4  3.8% respectively). A dose dependent induction of -catenin-mRNA could be demonstrated in cultured HFs after stimulation with 2.5 g/ml and 25 g/ml vitamin B 12 (fold change compared to DMEM: 9.5  2.7, p < 0.05 and 23.1  7.4, p < 0.01; respectively). Concomitantly the amounts of GSK-3 were significantly reduced after stimulation with 25 g/ml vitamin B 12 (fold change compared to DMEM: 0.76  0.12, p < 0.05). Conclusions: Our data demonstrate a hair growth promoting effect of vitamin B 12 in vitro. This effect is accompanied by the modulation of intracellular signal transduction molecules of the wnt-pathway and might promote hair growth after micrograft transplantation.

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The function of cellular transcobalamin II in cultured human cells

Cited by 9 publications

References 15 publications

Inherited Selective Intestinal Cobalamin Malabsorption and Cobalamin Deficiency in Dogs

Inherited Selective Intestinal Cobalamin Malabsorption and Cobalamin Deficiency in Dogs

Recent trends in the development of vitamin B₁₂derivatives for medicinal applications

Vitamin B12 Activates the Wnt-Pathway in Human Hair Follicle Cells by Induction of <i>beta</i>-Catenin and Inhibition of Glycogensynthase Kinase-3 Transcription

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The function of cellular transcobalamin II in cultured human cells

Cited by 9 publications

References 15 publications

Inherited Selective Intestinal Cobalamin Malabsorption and Cobalamin Deficiency in Dogs

Inherited Selective Intestinal Cobalamin Malabsorption and Cobalamin Deficiency in Dogs

Recent trends in the development of vitamin B12derivatives for medicinal applications

Vitamin B12 Activates the Wnt-Pathway in Human Hair Follicle Cells by Induction of &lt;i&gt;beta&lt;/i&gt;-Catenin and Inhibition of Glycogensynthase Kinase-3 Transcription

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Recent trends in the development of vitamin B₁₂derivatives for medicinal applications

Vitamin B12 Activates the Wnt-Pathway in Human Hair Follicle Cells by Induction of <i>beta</i>-Catenin and Inhibition of Glycogensynthase Kinase-3 Transcription