The Function of Haemoglobin in Chironomus Plumosus Under Natural Conditions

Abstract

1. The behaviour of final-instar larvae of Chironomus plumosus housed in U-shaped glass tubes was observed at various concentrations of dissolved oxygen and carbon dioxide. 2. Respiratory behaviour, consisting of intermittent irrigation of the tube, alternates with periods of filter-feeding or complete immobility. In well-aerated water about 50% of the time is occupied by respiratory behaviour, 35% by filter-feeding and the remainder by periods of rest. As the oxygen concentration in the water drops, progressively less time is occupied by filter-feeding and immobility and more by respiratory irrigation. Below 10% air saturation of the water larvae no longer feed. When placed in completely anaerobic conditions larvae at first irrigate intermittently but subsequently relapse into immobility. 3. During respiratory behaviour the amount of irrigation and the length of pauses between periods of irrigation change at different oxygen and carbon dioxide contents of the water in such a way as to suggest that the respiratory irrigation is controlled by internal pH changes in the larvae. 4. A spectroscopic examination of the haemoglobin in living larvae showed that the blood pigment holds an approximately 9-min. store of oxygen for the resting animal. In addition to this it acts in the transport of oxygen from the tube water to the larval tissues when the larva pauses between periods of irrigation. It thus decreases the amount of anaerobiosis to be endured during short periods of inactivity. Nevertheless, larvae without a functional haemoglobin (i.e. with carboxyhaemoglobin) still continue to pause during their respiratory behaviour, and the pauses are not strikingly curtailed in length. 5. At very low oxygen concentrations (7.5-9.0% air saturation), when the larva irrigates the tube almost unceasingly, the haemoglobin remains in a state of partial oxygenation, during which time it is functioning continuously in oxygen transport. At these oxygen concentrations larvae with carboxyhaemoglobin do not show respiratory activity but assume the immobility characteristic of anaerobic conditions. 6. Larvae with carboxyhaemoglobin tend to be less active than normal animals, except in well-aerated water, the decreased activity being largely due to a reduction in the amount of filter-feeding. Such larvae have not been observed to filter-feed at oxygen concentrations below 26% air saturation, whereas the limiting concentration for normal larvae is 10%. 7. After a prolonged period of anaerobiosis larvae show evidence of the repayment of an oxygen debt by prolonged irrigation of the tube when oxygen is once more available. A return to a normal irrigation rate is rapid and is usually followed by a period of filter-feeding The rate of recovery is proportional to the oxygen content of the incoming water, but normal larvae can recover even in water only 7 % air saturated. Larvae with carboxyhaemoglobin, on the other hand, show a considerably retarded rate of recovery from anaerobic conditions, and cannot recover in water less than 15% air saturated. 8. The main significance of haemoglobin in the life of a full-grown Chironomus larva would thus seem to be threefold: (a) haemoglobin enables the larva to maintain the active process of filter-feeding when relatively little oxygen is present; (b) it acts in oxygen transport at very low oxygen concentrations, thereby enabling continued respiratory irrigation; and (c) it greatly increases the rate of recovery from periods of oxygen lack, making such recovery possible even under adverse respiratory conditions.

Functional Hypoxia in Insects: Definition, Assessment, and Consequences for Physiology, Ecology, and Evolution

“…For example, aquatic P O 2 was found to vary tenfold in a shallow, sun-exposed pool (Figure 3), where increased temperature and consequent reduction in the solubility of O 2 and the increase in dissolved O 2 worked in tandem to create a hyperoxic P O 2 during the daytime (135). The few studies investigating direct effects of ambient hypoxia on aquatic insects have shown shifts in time budgets, with increased time spent on ventilation and concomitant reductions on other activities such as feeding (127,140). Aquatic insects differ widely in their ability to survive hypoxic exposure (127,133), and such differential survival likely underlies shifts in assemblage composition.…”

DNA Barcoding of Chironomid Larvae (Diptera: Chironomidae) from Large Rivers in South Korea to Facilitate Freshwater Biomonitoring and Public Health Surveillance

“…Furthermore, in line with our observations, Kim et al [34] reported that the genera Chironomus, Dicrotendipes, Glyptotendipes, Michrochironomus, Polypedilum, Stictochironomus, Tanytarsus, and Propsilocerus were most abundant in large South Korean rivers. Individuals in these genera can thrive in large rivers because of their adaptive larval and pupal traits such as hemoglobin production, and specific behaviors such as the construction of tubes and burrows in fine sediments [35][36][37]. Moreover, most of the species identified in our study are resistant to eutrophication, and some genera are highly resistant to hypoxia, a frequent phenomenon occurring in polluted streams and benthic zones in lentic areas of large rivers in Northeast Asia.…”

Effects of chironomid larvae density and mosquito biocide on methane and carbon dioxide dynamics in freshwater sediments

“…We sampled the headspace gas for measuring CH 4 and CO 2 concentrations through a connector in the lid and measured dissolved O 2 concentration in the water through contactless sensors (PyroScience GmbH, Germany) at 2 cm above the sediment surface. The relatively large headspace volume provided sufficient oxygen for maintaining the concentration of dissolved oxygen in the water well above 30% saturation, which has been reported as a threshold for alterations of the bioturbating activity of chironomid larvae [33,34]. To ensure gas equilibration between the water and the headspace, we continuously aerated the water with headspace air using an internal air pump and a submerged bubble diffusor (Fig 1).…”