The function of nuclear receptors in regulation of female reproduction and embryogenesis in the red flour beetle, Tribolium castaneum

Xu, Jingjing; Tan, Anjiang; Palli, Subba Reddy

doi:10.1016/j.jinsphys.2010.04.004

Cited by 104 publications

(79 citation statements)

References 49 publications

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“…Knockdown of seven nuclear receptors (E75, HR3, EcR, USP, SVP, FTZ-F1, and HR4) results in a significant reduction of vitellogenin prouction in T. castaneum (Xu et al, 2010), a phenotype similar to that obtained via TcMet knockdown (Parthasarathy & Palli, 2009). The data presented in this section therefore strongly support for the action of Met-like genes as crucial to 20E/JH crosstalk.…”

Section: Jh Regulation Of the E-20 Transcriptional Cascadesupporting

confidence: 70%

“…The Met-like genes of Tribolium and Drosophila appear to act in similar genetic environments to regulate the expression of members of the 20E induced transcriptional cascade, including EcR and USP (Xu et al, 2010), the heterodimeric components of the ecdysone receptor, various orphan nuclear receptors involved in 20E activity, and 20E-induced caspase genes involved in PCD (Liu et al, 2009). Knockdown of seven nuclear receptors (E75, HR3, EcR, USP, SVP, FTZ-F1, and HR4) results in a significant reduction of vitellogenin prouction in T. castaneum (Xu et al, 2010), a phenotype similar to that obtained via TcMet knockdown (Parthasarathy & Palli, 2009).…”

Section: Jh Regulation Of the E-20 Transcriptional Cascadementioning

confidence: 99%

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Molecular Evolution of Juvenile Hormone Signaling

Baumann¹,

Wilson²

2011

Gene Duplication

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Section: Jh Regulation Of the E-20 Transcriptional Cascadesupporting

confidence: 70%

Section: Jh Regulation Of the E-20 Transcriptional Cascadementioning

confidence: 99%

Molecular Evolution of Juvenile Hormone Signaling

Baumann¹,

Wilson²

2011

Gene Duplication

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“…Ecdysteroids signal oogenesis in Tribolium, Drosophila and mosquitoes (Culex pipiens) (Bernardi et al, 2009;Khater et al, 1994;Xu et al, 2010), but oogenesis is uncommon in worker bees because yolk uptake and oocyte maturation is suppressed pheromonally when a queen is present in the colony (Atkins et al, 1975). We found that three genes associated with the ecdysteroid cascade [HR46, ftz-f1 and ecdysone-induced protein 75 (E75)] were more highly expressed in Low pollen-hoarding strain ovaries than in the High strain.…”

Section: Ecdysteroid Hormones and Worker Ovarian Physiologymentioning

confidence: 99%

Regulation of behaviorally associated gene networks in worker honey bee ovaries

Wang

Kocher

Linksvayer

et al. 2012

Journal of Experimental Biology

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Fat body expression of hormone receptor-like in 46 (HR46) and phosphoinositide-dependent kinase-1 (PDK1) correlates with ovary size as well as worker division of labor (Wang et al., 2009). HR46 is a possible nuclear hormone receptor for ecdysteroid hormones Accepted 28 September 2011 SUMMARY Several lines of evidence support genetic links between ovary size and division of labor in worker honey bees. However, it is largely unknown how ovaries influence behavior. To address this question, we first performed transcriptional profiling on worker ovaries from two genotypes that differ in social behavior and ovary size. Then, we contrasted the differentially expressed ovarian genes with six sets of available brain transcriptomes. Finally, we probed behavior-related candidate gene networks in wild-type ovaries of different sizes. We found differential expression in 2151 ovarian transcripts in these artificially selected honey bee strains, corresponding to approximately 20.3% of the predicted gene set of honey bees. Differences in gene expression overlapped significantly with changes in the brain transcriptomes. Differentially expressed genes were associated with neural signal transmission (tyramine receptor, TYR) and ecdysteroid signaling; two independently tested nuclear hormone receptors (HR46 and ftz-f1) were also significantly correlated with ovary size in wild-type bees. We suggest that the correspondence between ovary and brain transcriptomes identified here indicates systemic regulatory networks among hormones (juvenile hormone and ecdysteroids), pheromones (queen mandibular pheromone), reproductive organs and nervous tissues in worker honey bees. Furthermore, robust correlations between ovary size and neural-and endocrine response genes are consistent with the hypothesized roles of the ovaries in honey bee behavioral regulation. Supplementary material available online at

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“…In the fruit fly Drosophila melanogaster, EcR mutants in females caused defects in oogenesis; the spectrum of oogenic defects includes the presence of abnormal egg chambers and disappearance of vitellogenic stages, indicating that EcR is required during the ovarian maturation of the species (Carney & Bender 2000). It has also been reported that the knockdown of the EcR gene by RNA interference (RNAi) significantly reduced the level of vitellogenin (Vg) mRNA in the red flour beetle Tribolium castaneum, indicating that EcR is required for primary oocyte maturation, ovarian growth, and the migration of the follicle cells of this specie (Parthasarathy et al 2010, Xu et al 2010. In crustaceans, ecdysteroids can induce the expression of the Vg gene and the concentrations of ecdysteroids increase during the initial stages of oogonial and spermatogonial mitoses (Subramoniam 2000, Tiu et al 2010.…”

Section: Introductionmentioning

confidence: 99%

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

Gong¹,

Ye²,

Xie³

et al. 2015

Journal of Endocrinology

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In arthropods, it is known that ecdysteroids regulate molting, limb regeneration, and reproduction through activation of the ecdysone receptor (EcR). However, the ecdysteroid signaling pathway for promotion of ovarian development in crustaceans is still unclear. In this study, three cDNA isoforms of EcR were cloned from the mud crab Scylla paramamosain. qRT-PCR revealed that the SpEcR mRNA was abundant in the eyestalk, ovary and epidermis. During ovarian development, the SpEcR transcripts increased from stage I (undeveloped stage) and reached a peak at stage IV (late vitellogenic stage) before dropping to a lower level at stage V (mature stage). Meanwhile, levels of 20-hydroxyecdysone (20E) in the hemolymph, detected by HPLC-MS, displayed a similar pattern of increase with ovarian development. Results from in situ hybridization indicated that SpEcR mRNA was present in the follicular cells during vitellogenesis. Results from in vivo experiments revealed that 20E at 0.2 mg/g body weight significantly stimulated the expression of SpEcR and vitellogenin (SpVg) in female crabs during the early vitellogenic stage but not during the previtellogenic stage. This was confirmed by results from in vitro experiments which indicated that SpEcR and SpVg expression levels were significantly upregulated in early vitellogenic ovarian explants incubated with 5.0 mM 20E at 3 and 6 h but not in previtellogenic ovarian explants. Finally, results from in vitro gene silencing experiments indicated that the expression of SpEcR and SpVg in the ovary was significantly inhibited by SpEcR dsRNA. All these results together indicated that in S. paramamosain, 20E, and SpEcR, located in the follicular cells, play important roles in the promotion of ovarian development via regulating the expression of SpVg.

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The function of nuclear receptors in regulation of female reproduction and embryogenesis in the red flour beetle, Tribolium castaneum

Cited by 104 publications

References 49 publications

Molecular Evolution of Juvenile Hormone Signaling

Molecular Evolution of Juvenile Hormone Signaling

Regulation of behaviorally associated gene networks in worker honey bee ovaries

Ecdysone receptor in the mud crab Scylla paramamosain: a possible role in promoting ovarian development

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