The Functional Integrity of a Normothermic Perfusion System Using Artificial Blood in Pig Liver

Nui, Akihiro; Kimura, Tadashi; Kikuchi, Hitoshi; Kukita, Kazuma; Kimura, Hitoshi; Meguro, Makoto; Nagayama, Masatoshi; Isobe, Masato; Hirata, Koichi

doi:10.1016/j.jss.2005.11.579

Cited by 5 publications

(5 citation statements)

References 29 publications

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“…One foreseeable trend is the use of an artificial oxygen carrier as a substitution for packed RBCs [67]. Thereby, the infectious risk factor could be reduced and all components of the perfusate would be easily accessible and storable.…”

Section: Future Trendsmentioning

confidence: 99%

Early Clinical Results Using Normothermic Machine Liver Preservation

et al. 2015

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The ongoing shortage of donor organs for liver transplantation results in high mortality rates for those on the waiting list. This has triggered considerable interest in the use of marginal liver grafts to increase the donor pool. Unfortunately, marginal grafts poorly tolerate preservation by cold storage (CS), resulting in an increased risk of liver injury and poor graft function after transplantation. Better preservation techniques are needed to fully utilize marginal grafts and to expand the number of organ donors. Animal studies have demonstrated normothermic ex vivo liver perfusion to be superior to conventional CS. Recently, the first clinical trial of normothermic ex vivo liver perfusion was completed with promising results. Similarly, in situ recirculation after cardiac arrest as the initial preservation method before organ procurement has proven to be a valuable technique to make more liver grafts available for transplantation. Though technically more complex and economically more expensive, normothermic liver perfusion is a promising and innovative technique that is able to positively impact current transplant practice and outcomes.

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Section: Future Trendsmentioning

confidence: 99%

Early Clinical Results Using Normothermic Machine Liver Preservation

et al. 2015

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“…Naturally, an isolated organ is the sole contributor to changes in metabolite concentrations, which allows these changes to be attributed to organ-specific influences. This can be exploited to study the effects of certain interventions in basic metabolic pathways 132 or the metabolism of a target compound 133,134 . Additionally, the ease of in- and outflow sampling allows for metabolic flux analysis over the organ and the evaluation of metabolic pathways over time and between groups 33,135 .…”

Section: Applicationsmentioning

confidence: 99%

Organomatics and organometrics: Novel platforms for long-term whole-organ culture

2014

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Organ culture systems are instrumental as experimental whole-organ models of physiology and disease, as well as preservation modalities facilitating organ replacement therapies such as transplantation. Nevertheless, a coordinated system of machine perfusion components and integrated regulatory control has yet to be fully developed to achieve long-term maintenance of organ function ex vivo. Here we outline current strategies for organ culture, or organomatics, and how these systems can be regulated by means of computational algorithms, or organometrics, to achieve the organ culture platforms anticipated in modern-day biomedicine.

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“…These data were previously obtained in larger animal models, by Schon et al in 2001 (33), who transplanted livers perfused at normothermia in a porcine model, and by Butler et al in 2002 (34), who maintained viable pig livers perfused at normothermia for 72 h. Other studies have subsequently confirmed that normothermic perfusion not only better preserves livers but also revitalizes NHBD livers (DCDD) (35)(36)(37)(38)(39). However, theoretically, normothermic perfusion has greater possibility of infection, so there is a need for the administration of antibiotics and for an oxygen carrier as well (40,41), which could limit the possibility of clinical application. The only limitation to the use of normothermic machine perfusion is the cost due to a more complex organization.…”

Section: Hypothermia Subnormothermia and Normothermiamentioning

confidence: 99%

“…Henry et al (15) highlighted the lack of clarity regarding whether HMP requires active oxygenation or diffusion alone can maintain usable levels of oxygen, and Olschewski et al (43) pointed out the lack of a specific oxygen transporter that causes liver cell damage due to oxygen deficiency in perfusion tests at 21 °C. For effective result, the liver must be perfused by blood (40,34), or by a fluid with an artificial carrier (41). Majority of arterial oxygen is transported in the form of oxyhemoglobin, and a small percentage (0.3%) is dissolved in the plasma.…”

Section: Oxygenation Of the Perfusion Solutionmentioning

confidence: 99%

Perfusion machines for liver transplantation: technology and multifunctionality

Rubbini

2013

Updates Surg

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The reliability of machine perfusion (alternative to static cold storage) for the preservation of liver for transplantation has been well investigated in experimental models, by taking into account the temperature, oxygenation, flow, pressure, and settings of the machine or proposed circuit. Machine perfusion is considered by many researchers as a valid method for preserving organs. While circuits or machines for preservation have been described, no agreement has been reached concerning how these devices should be developed. The machines proposed to date are considered here to identify the technical and functional features necessary for a machine to have multifunctionality and adaptability to cater all the needs of preservation, according to the type and features of the liver to be transplanted, including marginal livers. The need to establish a uniform method for the use of this machine is also emphasized, to achieve a clinical protocol for its use.

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The Functional Integrity of a Normothermic Perfusion System Using Artificial Blood in Pig Liver

Cited by 5 publications

References 29 publications

Early Clinical Results Using Normothermic Machine Liver Preservation

Early Clinical Results Using Normothermic Machine Liver Preservation

Organomatics and organometrics: Novel platforms for long-term whole-organ culture

Perfusion machines for liver transplantation: technology and multifunctionality

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