The Functional Role of PMP22 Gene in the Proliferation and Invasion of Osteosarcoma

Abstract

BackgroundAs the most common primary bone tumor, osteosarcoma has an improved survival rates with advancement of treatment methods. A higher rate of metastasis, however, leads to the aggravation of the disease. Studies have shown that some genes, namely osteosarcoma metastasis-related genes, participate in the process of tumor metastasis. The peripheral myelin protein 22 (PMP22) gene has recently been found to be abundantly expressed in the oncogenesis of osteosarcoma. Its detailed role and function in the tumor metastasis, however, remains unknown.Material/MethodsThe recombinant retroviral plasmid pcDNA3.1-PMP22 was constructed and used to transfect osteosarcoma cells SOSP-M, whose cell proliferation was measured by MTT method. The formation of tumor cell colony, the cell migration and invasion were also measured. The signal transduction pathway MAPK was further analyzed by Western blotting.ResultsThe pcDNA3.1-PMP22 plasmid was confirmed to have a 305bp PMP22 fragment by EcoRI-XhoI dual digestion. Compared to the control group, osteosarcoma cell invasion was significantly facilitated by the transfection of pcDNA3.1-PMP22 plasmid (p<0.05). The recombinant plasmid also significantly potentiated the formation of tumor cell colony and increased the migration and invasion ability of tumor cells (p<0.05 in all cases). Phosphorylated p-ERK and p-P38 were also up-regulated by vector transfection (p<0.05).ConclusionsOsteosarcoma metastasis-related gene PMP22 participates in the proliferation, invasion, migration and colony formation of osteosarcoma cells possibly via the MAPK signal transduction pathway, providing evidences for further investigation of metastatic mechanism of osteosarcoma.

Leptocarpin Suppresses Proliferation, Migration, and Invasion of Human Osteosarcoma by Targeting Type-1 Insulin-Like Growth Factor Receptor (IGF-1R)

“…To confirm the promotion of overexpressed IGF-1R on tumor cell proliferation, we referred to previous studies [ 16 , 17 ] and detected the IGF-1R expression in MG63 cells. We found that LTC treatment obviously inhibited IGF-1R expression in MG63 cells, similar to results of siRNA silencing IGF-1R.…”

“…At the genetic level, previous research [ 15 ] has shown that many genetic and epigenetic agents are involved in tumor pathogenesis and progression. According to Liu et al [ 16 ], PMP22 (peripheral myelin protein 22) was found to be overexpressed in osteosarcoma. The in vivo experiments proved the relationship between PMP22 and the proliferation, migration, invasion, and colony formation of osteosarcoma cells.…”

MicroRNA-184 Modulates Doxorubicin Resistance in Osteosarcoma Cells by Targeting BCL2L1

“…Osteosarcoma (OS) is an important cause of cancer-correlated deaths worldwide. OS progression is a complex, multistep process involving numerous abnormal genes and proteins [ 20 , 21 ]. The combination of local radiotherapy/chemotherapy and surgery is currently the main anti-tumor therapy, but treatment fails in many patients, in part due to resistance to chemotherapeutic agents [ 22 , 23 ].…”

Identification of key biomarkers involved in osteosarcoma using altered modules

“…Recent studies have mainly focused on individual gene-related analyses (Rosemann et al, 2014;Liu and Chen, 2015;Zeng and Xu, 2015;Li et al, 2016). However, these do not accurately reflect the interaction between integral genes under different conditions (Slonim, 2002).…”