The Functions of B-Vitamins in the Biosynthesis of Purines and Pyrimidines

Shive, William

doi:10.1016/s0083-6729(08)60470-7

Cited by 30 publications

(3 citation statements)

References 270 publications

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“…The results presented here serve to substantiate the original postulation that p-aminobenzoic acid acts as a precursor to a functional derivative which is required, among other things, for the synthesis of purines via 4-amino-5-imidazolecarboxamide, or its close derivative (Shive, 1950(Shive, , 1951Woods, 1950). This conclusion is based on the fact that the accumulation of 4-amino-5-midazolecarboxamide occurs when there is an interference with (1) the synthesis of paminobenzoic acid as represented by the genetic block in the p-aminobenzoic auxotroph, and (2) the utilization of p-aminobenzoic acid as occurs under the competitive inhibition by the analogues of p-aminobenzoic acid.…”

Section: Discussionsupporting

confidence: 73%

Studies on Purine Metabolism in Bacteria I

Gots

Chu

1952

J Bacteriol

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The evidence that p-aminobenzoic acid functions in purine synthesis was originally suggested by nutritional studies and by systems involving inhibition analysis as recently reviewed by Shive (1951). The occurrence of 4-amino-5imidazolecarboxamide in sulfonamide inhibited cultures of Escherichia coli offered a lead in localizing the site of p-aminobenzoic acid involvement in purine synthesis (Stetten and Fox, 1945; Shive et at., 1947). It was postulated that I This research has been aided by a grant from the Damon Runyon Fund through the American Cancer Society as recommended by the Committee on Growth of the National Research Council, and also by a grant from the National Foundation for Infantile Paralysis.

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Section: Discussionsupporting

confidence: 73%

Studies on Purine Metabolism in Bacteria I

Gots

Chu

1952

J Bacteriol

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“…The increased quantitative requirement for p-aminobenzoic acid (or Leucovorin) in growth under conditions where serine must be derived from glycine clearly indicates a specific function of folic acid a t this step in serine synthesis. The ability of certain mixtures of amino acids and nucleic acid derivatives to replace the growth factor function of p-aminobenzoic acid and pteroylglutamic acid (and the anti-sulphonamide function of the former) had earlier suggested that Nutrition of Leuconostoc mesenteroides P 60 folic acid was required at some stage in serine formation (see reviews by Shive, 1951 ;Woods, 1952); serine was specifically implicated by Lampen, Jones & Roepke (1949) and Winkler & de Haan (1948) in the case of p-aminobenzoic acid and by Broquist & Snell (see Snell, 1951) in the case of pteroylglutamic acid. Wold & Sirny (1953) briefly reported that Leucovorin will replace serine for the growth of Ln.…”

Section: Discussionmentioning

confidence: 99%

The Folic Acid and Serine Nutrition of Leuconostoc mesenteroides P60 (Streptococcus equinus P60)

Lascelles

Woods

1954

Journal of General Microbiology

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SUMMARY : Leuconostoc mesenteroides P 60 (Streptococcus equinus P 60) requires serine for growth under normal conditions. On a medium containing relatively high concentrations of glycine it can dispense with serine provided that ( a ) pyridoxal is present, and (b) either Leucovorin (folinic acid) is added or the atmosphere is enriched with CO, . Increasing concentrations of CO, or Leucovorin decrease the concentration of glycine required to support growth without added serine. Pyridoxal is not necessary for optimal growth with added serine, but the required concentration of the latter is twice as great.Higher concentrations of p-aminobenzoic acid or Leucovorin are required for growth on glycine than on serine. Leucovorin replaces p-aminobenzoic acid for growth on serine, and both this factor and CO, for growth on glycine. With the basal medium used (containing purines and all other amino-acids) thymidine supports growth in the absence of p-aminobenzoic acid only when serine is added.Growth is not inhibited by sulphonamide when Leucovorin is present ; with p-aminobenzoic acid there is the usual competition. Pteroylglutamic acid is inactive with this organism.A valid assay for serine is possible, even with high concentrations of glycine present, when precautions are taken t o destroy either Leucovorin or pyridoxal in the samples, and to prevent the atmosphere becoming enriched with CO,.

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“…In recent years involvement of formate carbon in the synthesis of a variety of metabolites has become well established (Shive, 1951). With few exceptions the metabolites have been nitrogen containing compounds.…”

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confidence: 99%