The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

Liu, Gongyan; Li, Shu; Liu, Hongli; Zhu, Yanli; Bai, Liya; Sun, Haitao; Gao, Shuxia; Jiang, Wenxue; Li, Fuchang

doi:10.1186/s12861-020-00213-5

Cited by 18 publications

(10 citation statements)

References 51 publications

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“…Previous studies have shown that there is a direct relationship between the Notch signal pathway and hair follicle morphogenesis (Lin et al, 2000), high expression of Notch1 and Notch2 can accelerate the formation of mouse hair substrate, and at the same time, it can inhibit the cells around the hair substrate to form substrate (Crowe et al, 1998). Ocu-miR-205 can promote the transition of Rex rabbit hair follicles from the growing stage to a degenerative quiescent stage by regulating the expression of related genes and proteins in Notch, BMP, and other signaling pathways, to change the hair density (Liu et al, 2020). Krieger used a mouse model to study the regulation of the NF-kappa B signaling pathway in the hair follicle cycle and found that the NF-kappa B signaling pathway is essential for the growth and activation of hair follicle stem cells (Krieger et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goat Hair Follicles

Shang

Wang²,

Ma³

et al. 2021

Front. Genet.

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BackgroundInner Mongolian cashmere goats have hair of excellent quality and high economic value, and the skin hair follicle traits of cashmere goats have a direct and important effect on cashmere yield and quality. Circular RNA has been studied in a variety of tissues and cells.ResultIn this study, high-throughput sequencing was used to obtain the expression profiles of circular RNA (circRNA) in the hair follicles of Inner Mongolian cashmere goats at different embryonic stages (45, 55, 65, and 75 days). A total of 21,784 circRNAs were identified. At the same time, the differentially expressed circRNA in the six comparison groups formed in the four stages were: d75vsd45, 59 upregulated and 33 downregulated DE circRNAs; d75vsd55, 61 upregulated and 102 downregulated DE circRNAs; d75vsd65, 32 upregulated and 33 downregulated DE circRNAs; d65vsd55, 67 upregulated and 169 downregulated DE circRNAs; d65vsd45, 96 upregulated and 63 downregulated DE circRNAs; and d55vsd45, 76 upregulated and 42 downregulated DE circRNAs. Six DE circRNA were randomly selected to verify the reliability of the sequencing results by quantitative RT-PCR. Subsequently, the circRNA corresponding host genes were analyzed by the Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. The results showed that the biological processes related to hair follicle growth and development enriched by GO mainly included hair follicle morphogenesis and cell development, and the signaling pathways related to hair follicle development included the Notch signaling pathway and NF-κB signaling pathway. We combined the DE circRNA of d75vsd45 with miRNA and mRNA databases (unpublished) to construct the regulatory network of circRNA–miRNA–mRNA, and formed a total of 102 pairs of circRNA–miRNA and 126 pairs of miRNA–mRNA interactions. The binding relationship of circRNA3236–chi-miR-27b-3p and circRNA3236–chi-miR-16b-3p was further verified by dual-luciferase reporter assays, and the results showed that circRNA3236 and chi-miR-27b-3p, and circRNA3236 and chi-miR-16b-3p have a targeted binding relationship.ConclusionTo summarize, we established the expression profiling of circRNA in the fetal skin hair follicles of cashmere goats, and found that the host gene of circRNA may be involved in the development of hair follicles of cashmere goats. The regulatory network of circRNA–miRNA–mRNA was constructed and preliminarily verified using DE circRNAs.

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Section: Discussionmentioning

confidence: 99%

Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goat Hair Follicles

Shang

Wang²,

Ma³

et al. 2021

Front. Genet.

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“…These lncRNAs and genes might play crucial roles in regulating hair follicle density, and their differential expression might be the reason for differences in hair follicle density and wool production between HWP and LWP rabbits. Liu et al (2020) analysed the miRNA effect on hair follicle density in the Rex rabbit [37], but lncRNA related to hair density in rabbits has only been done in the present study.…”

Section: Discussionmentioning

confidence: 90%

Analysis of Histology and Long Noncoding RNAs Involved in the Rabbit Hair Follicle Density using RNA Sequencing

Ding

Zhao

et al. 2020

Preprint

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Background: Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive. Results: In this study, hair follicle density at different body sites of Wan strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR). Conclusions: LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.

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“…These lncRNAs and genes might play crucial roles in regulating hair follicle density, and their differential expression might be the reason for differences in hair follicle density and wool production between HWP and LWP rabbits. Liu et al (2020) analysed the miRNA effect on hair follicle density in the Rex rabbit [ 35 ], but lncRNA related to hair density in rabbits has only been done in the present study.…”

Section: Discussionmentioning

confidence: 98%

Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

Ding

Zhao

et al. 2021

BMC Genomics

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Background Hair follicle density influences wool fibre production, which is one of the most important traits of the Wan Strain Angora rabbit. However, molecular mechanisms regulating hair follicle density have remained elusive. Results In this study, hair follicle density at different body sites of Wan Strain Angora rabbits with high and low wool production (HWP and LWP) was investigated by histological analysis. Haematoxylin-eosin staining showed a higher hair follicle density in the skin of the HWP rabbits. The long noncoding RNA (lncRNA) profile was investigated by RNA sequencing, and 50 and 38 differentially expressed (DE) lncRNAs and genes, respectively, were screened between the HWP and LWP groups. A gene ontology analysis revealed that phospholipid, lipid metabolic, apoptotic, lipid biosynthetic, and lipid and fatty acid transport processes were significantly enriched. Potential functional lncRNAs that regulate lipid metabolism, amino acid synthesis, as well as the Janus kinase (JAK)-signal transducer and activator of transcription (STAT) and hedgehog signalling pathways, were identified. Consequently, five lncRNAs (LNC_002171, LNC_000797, LNC_005567, LNC_013595, and LNC_020367) were considered to be potential regulators of hair follicle density and development. Three DE lncRNAs and genes were validated by quantitative real-time polymerase chain reaction (q-PCR). Conclusions LncRNA profiles provide information on lncRNA expression to improve the understanding of molecular mechanisms involved in the regulation of hair follicle density.

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The functions of ocu-miR-205 in regulating hair follicle development in Rex rabbits

Cited by 18 publications

References 51 publications

Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goat Hair Follicles

Expression Profiling and Functional Analysis of Circular RNAs in Inner Mongolian Cashmere Goat Hair Follicles

Analysis of Histology and Long Noncoding RNAs Involved in the Rabbit Hair Follicle Density using RNA Sequencing

Analysis of histology and long noncoding RNAs involved in the rabbit hair follicle density using RNA sequencing

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