The fungicide iprodione affects midgut cells of non-target honey bee Apis mellifera workers

Carneiro, Lenise Silva; Martínez, Luis Carlos; Gonçalves, Wagner Gonzaga; Santana, Luanda Medeiros; Serrão, José Éduardo

doi:10.1016/j.ecoenv.2019.109991

Cited by 72 publications

(32 citation statements)

References 68 publications

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“…The histological changes found are characteristic of a degenerative cellular process, such as disorganization of the striated border, cytoplasm vacuolization, and cell fragmentation. In the midgut, cell degeneration is described in other insects, such as Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae) in response to chlorpyrifos [ 50 ] and chlorantraniliprole [ 51 ], Apis mellifera Linnaeus (Hymenoptera: Apidae) to iprodione [ 56 ] and spiromesifen [ 57 ], Podisus nigrispinus Dallas (Hemiptera: Pentatomidae) to permethrin [ 21 ] and spinosad [ 22 ], and Aedes aegypti (Diptera: Culicidae) to pyriproxyfen [ 30 ]. The histo-toxic effects in the midgut of S. frugiperda exposed to deltamethrin are mainly damages in the striated border of the digestive cells and in the peritrophic matrix.…”

Section: Discussionmentioning

confidence: 99%

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Vinha

Plata-Rueda

Soares

et al. 2021

Insects

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Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) is the main pest of maize crops, and effective methods for pest management are needed. The insecticidal efficacy of deltamethrin was evaluated against S. frugiperda for toxicity, survival, locomotion, anti-feeding, and histological changes in the midgut. Concentration–mortality bioassays confirmed that deltamethrin (LC50 = 3.58 mg mL−1) is toxic to S. frugiperda caterpillars. The survival rate was 99.7% in caterpillars not exposed to deltamethrin, decreasing to 50.3% in caterpillars exposed to LC50, and 0.1% in caterpillars treated with LC90. Spodoptera frugiperda demonstrated reduced mobility on deltamethrin-treated surfaces. Deltamethrin promoted a low respiration rate of S. frugiperda for up to 3 h after insecticide exposure, displaying immobilization and inhibiting food consumption. Deltamethrin induces histological alterations (e.g., disorganization of the striated border, cytoplasm vacuolization, and cell fragmentation) in the midgut, damaging the digestive cells and peritrophic matrix, affecting digestion and nutrient absorption.

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Section: Discussionmentioning

confidence: 99%

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Vinha

Plata-Rueda

Soares

et al. 2021

Insects

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“…Systemic infections in honey bees could be exacerbated by acaricide, neonicotinoid, or fungicide exposure, which reduces intestinal stem cell proliferation (Forkpah et al, 2014) and increases midgut apoptosis (Gregorc et al, 2018;Carneiro et al, 2019), potentially weakening the gut barrier. Hemocytes also function as phagocytic cells in the honey bee hemolymph; however exposure to neonicotinoids reduces hemocytes phagocytic activity (Walderdorff et al, 2018) and hemolymph antimicrobial activity (Brandt et al, 2016).…”

Section: Pesticides Disrupt Honey Bee Immunitymentioning

confidence: 99%

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

et al. 2020

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Managed populations of the European honey bee (Apis mellifera) support the production of a global food supply. This important role in modern agriculture has rendered honey bees vulnerable to the noxious effects of anthropogenic stressors such as pesticides. Although the deleterious outcomes of lethal pesticide exposure on honey bee health and performance are apparent, the ominous role of sublethal pesticide exposure is an emerging concern as well. Here, we use a data harvesting approach to better understand the toxicological effects of pesticide exposure across the honey bee life cycle. Through compiling adult-and larval-specific median lethal dose (LD 50) values from 93 published data sources, LD 50 estimates for insecticides, herbicides, acaricides, and fungicides are highly variable across studies, especially for herbicides and fungicides, which are underrepresented in the meta-data set. Alongside major discrepancies in these reported values, further examination of the compiled data suggested that LD 50 may not be an ideal metric for honey bee risk assessment. We also discuss how sublethal effects of pesticide exposure, which are not typically measured in LD 50 studies, can diminish honey bee reproduction, immunity, cognition, and overall physiological functioning, leading to suboptimal honey bee performance and population reduction. In consideration of actionable solutions to mitigate the effects of sublethal pesticide exposure, we have identified the potential for probiotic supplementation as a promising strategy that can be easily incorporated alongside current agricultural infrastructure and apicultural management practices. Probiotic supplementation is regularly employed in apiculture but the potential for evidence-based targeted approaches has not yet been fully explored within a formal toxicological context. We discuss the benefits, practical considerations, and limitations for the use and delivery of probiotics to hives. Ultimately, by subverting the sublethal effects of pesticides we can help improve the long-term survival of these critical pollinators.

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“…Fungicides are the main agrochemicals found in pollen grains, which are the principal source of protein for bees (Carneiro et al, 2020). Although the effects of insecticides on bees are widely studied, there is growing evidence that fungicides are also not harmless to bees (Batista et al, 2020).…”

Section: Midgut Morphological Analysismentioning

confidence: 99%

“…The results indicated that after five days of exposure, the fungicide induced sublethal effects in the midgut of adult workers. Carneiro et al (2020) also evaluated the cytotoxic effects of oral contamination of A. mellifera with the fungicide iprodione and observed the impairment of the cells of the midgut that showed signs of apoptosis.…”

Section: Midgut Morphological Analysismentioning

confidence: 99%

Effects of combined fungicide in stingless bees Scaptotrigona bipunctata

Diniz

Pereira

Silva

et al. 2021

RSD

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Locker is a fungicide used in different crops for biological control, affecting both insect pests and pollinators, such as bees. This study aimed to evaluate toxicity and establish the lethal concentration (LC50) of the fungicide Locker in stingless bees Scaptotrigona bipunctata, investigating changes in brain cells chromatin structure and in midgut morphology. This agrochemical is a combined fungicide that contains three active ingredients: benzimidazole, triazole and strobilurin. Adult forage bees were orally exposed to the following concentrations: 1.7 mg a.i./L; 2.55 mg a.i./L; 3.4 mg a.i./L and 4.25 mg a.i./L of fungicide, and submitted to histochemical and morphological analysis 24, 48 and 72 hours after ingestion. The analysis of S. bipunctata brain cells showed changes in chromatin condensation in all treatments. There were also several morphological changes in the midgut at all concentrations and exposure times. Thus, the fungicide Locker significantly affected bee survival, inducing changes in their internal morphology, which can lead to alterations in their activities, directly interfering in their foraging behavior and survival.

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The fungicide iprodione affects midgut cells of non-target honey bee Apis mellifera workers

Cited by 72 publications

References 68 publications

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Deltamethrin-Mediated Effects on Locomotion, Respiration, Feeding, and Histological Changes in the Midgut of Spodoptera frugiperda Caterpillars

Understanding the Effects of Sublethal Pesticide Exposure on Honey Bees: A Role for Probiotics as Mediators of Environmental Stress

Effects of combined fungicide in stingless bees Scaptotrigona bipunctata

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