The fungicide propamocarb increases lignin by activating the phenylpropanoid pathway in Cucumis sativus L.

Wu, Ping; Guo, Qianqian; Qin, Zhiwei

doi:10.1007/s13580-016-0049-1

Cited by 18 publications

(12 citation statements)

References 29 publications

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“…In the phenylpropanoid pathway, we identified seven upregulated DEGs and four DEGs with mixed expression patterns in inoculated seedlings exposed to salt. Among these DEGs, we detected DEGs encoding PAL and POD, two key enzymes for lignin biosynthesis, which catalyze the initial and final synthesis steps, respectively (56). Lignin is a phenylpropanoid compound derived from phenylalanine.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Bacterial Microbiota of Coastal Halophyte Limonium sinense and Amelioration of Salinity Stress Damage by Symbiotic Plant Growth-Promoting Actinobacterium Glutamicibacter halophytocola KLBMP 5180

Qin

Feng

Zhang

et al. 2018

Appl Environ Microbiol

105

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Plant-associated microorganisms are considered a key determinant of plant health and growth. However, little information is available regarding the composition and ecological function of the roots' and leaves' bacterial microbiota of halophytes. Here, using both culture-dependent and culture-independent techniques, we characterized the bacterial communities of the roots and leaves as well as the rhizosphere and bulk soils of the coastal halophyte in Jiangsu Province, China. We identified 49 representative bacterial strains belonging to 17 genera across all samples, with as the most dominant genus. All isolates showed multiple potential plant growth promotion traits and tolerated a high concentration of NaCl and a wide pH range. Interestingly, further inoculation experiments showed that the strain KLBMP 5180 isolated from root tissue significantly promoted host growth under NaCl stress. Indeed, KLBMP 5180 inoculation increased the concentrations of total chlorophyll, proline, antioxidative enzymes, flavonoids, K, and Ca in the leaves; the concentrations of malondialdehyde (MDA) and Na were reduced. A transcriptome analysis identified 1,359 and 328 differentially expressed genes (DEGs) in inoculated seedlings treated with 0 and 250 mM NaCl, respectively. We found that pathways related to phenylpropanoid and flavonoid biosynthesis and ion transport and metabolism might play more important roles in host salt stress tolerance induced by KLBMP 5180 inoculation compared to that in noninoculated leaves. Our results provide novel insights into the complex composition and function of the bacterial microbiota of the coastal halophyte and suggest that halophytes might recruit specific bacteria to enhance their tolerance of harsh environments. Halophytes are important coastal plants often used for the remediation of saline coastal soils. is well known for its medical properties and phytoremediation of saline soils. However, excessive exploitation and utilization have made the wild resource endangered. The use of endophytic and rhizosphere bacteria may be one of the suitable ways to solve the problem. This study was undertaken to develop approaches to improve the growth of using endophytes. The application of actinobacterial endophytes ameliorated salt stress damage of the host via complex physiological and molecular mechanisms. The results also highlight the potential of using habitat-adapted, symbiotic, indigenous endophytic bacteria to enhance the growth and ameliorate abiotic stress damage of host plants growing in special habitats.

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Section: Discussionmentioning

confidence: 99%

Diversity of Bacterial Microbiota of Coastal Halophyte Limonium sinense and Amelioration of Salinity Stress Damage by Symbiotic Plant Growth-Promoting Actinobacterium Glutamicibacter halophytocola KLBMP 5180

Qin

Feng

Zhang

et al. 2018

Appl Environ Microbiol

105

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“…This pathway is significantly associated with the cucumber metabolism of PM [ 21 ]. POD activities increased in cultivar ‘D0351’ after treatment with PM, while lignin increased by 41.1% [ 45 ]. Here, we found that POD activity in CsDIR16 (+) T 1 transgenic plants fell more rapidly compared with that in wild-type plants, indicating that CsDIR16 could increase POD-activity responses to PM.…”

Section: Discussionmentioning

confidence: 99%

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

et al. 2018

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BackgroundCucumber downy mildew is among the most important diseases that can disrupt cucumber production. Propamocarb, also known as propyl-[3-(dimethylamino)propyl]carbamate (PM), is a systemic carbamate fungicide pesticide that is widely applied in agricultural production because of its high efficiency of pathogens control, especially cucumber downy mildew. However, residual PM can remain in cucumbers after the disease has been controlled. To explore the molecular mechanisms of PM retention, cucumber cultivars ‘D9320’ (with the highest residual PM content) and ‘D0351’ (lowest residual PM content) were studied. High-throughput tag-sequencing (Tag-Seq) results showed that the CsDIR16 gene was related to PM residue, which was verified using transgenic technology.ResultsWe investigated the activity of a dirigent cucumber protein encoded by the CsDIR16 in gene response to stress induced by PM treatment. Gene-expression levels of CsDIR16 were up-regulated in the fruits, leaves, and stems of ‘D0351’ plants in response to PM treatment. However, in cultivar ‘D9320’, CsDIR16 levels were down-regulated in the leaves and stems after PM treatment, with no statistically significant differences observed in the fruits. Induction by jasmonic acid, abscisic acid, polyethylene glycol 4000, NaCl, and Corynespora cassiicola Wei (Cor) resulted in CsDIR16 up-regulation in ‘D0351’ and ‘D9320’. Expression after salicylic acid treatment was up-regulated in ‘D0351’, but was down-regulated in ‘D9320’. CsDIR16 overexpression lowered PM residues, and these were more rapidly reduced in CsDIR16(+) transgenic ‘D9320’ plants than in wild-type ‘D9320’ and CsDIR16(−) transgenic plants.ConclusionsAnalyses of the CsDIR16-expression patterns in the cucumber cultivars with the highest and lowest levels of PM residue, and transgenic validation indicated that CsDIR16 plays a positive role in reducing PM residues. The findings of this study help understand the regulatory mechanisms occurring in response to PM stress in cucumbers and in establishing the genetic basis for developing low-pesticide residue cucumber cultivars.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1236-2) contains supplementary material, which is available to authorized users.

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“…Phenylalanine ammonia-lyase (PAL) produces precursors of various secondary metabolites, including lignin, phytoalexin, and phenolic compounds. This gene family is also associated with the production of the first enzyme of the phenylpropanoid pathway [1][2][3]. PAL genes have a molecular mass in the range of 270-330 kilodalton (kDa) and are present in higher plants, yeast, some bacteria, and fungi.…”

Section: Introductionmentioning

confidence: 99%

“…The conversion of L-phenylalanine to cinnamic acid, linking primary metabolism with secondary metabolism catalyzed by the PAL enzymes, also plays an essential role in phenylpropanol biosynthesis, a speed-limiting step in phenylpropanol metabolism [1].This metabolic pathway is involved in the production of various natural products (phytoalexin, hydroxycinnamic acids, flavonoids, etc. ), and is also reported as a role player in phenolic glycoside and benzene compound synthesis, which are part of several enzyme-regulated reactions [1,2,[7][8][9][10]. Thus, phenylpropanoids play a critical role for the growth, development, and survival of vascular plants [1].…”

Section: Introductionmentioning

confidence: 99%

Phenylalanine Ammonia-Lyase (PAL) Genes Family in Wheat (Triticum aestivum L.): Genome-Wide Characterization and Expression Profiling

Rasool

Uzair²,

Naeem³

et al. 2021

Agronomy

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Phenylalanine ammonia-lyase (PAL) is the first enzyme in the phenylpropanoid pathway and plays a vital role in adoption, growth, and development in plants but in wheat its characterization is still not very clear. Here, we report a genome-wide identification of TaPAL genes and analysis of their transcriptional expression, duplication, and phylogeny in wheat. A total of 37 TaPAL genes that cluster into three subfamilies have been identified based on phylogenetic analysis. These TaPAL genes are distributed on 1A, 1B, 1D, 2A, 2B, 2D, 4A, 5B, 6A, 6B, and 6D chromosomes. Gene structure, conserved domain analysis, and investigation of cis-regulatory elements were systematically carried out. Chromosomal rearrangements and gene loss were observed by evolutionary analysis of the orthologs among Triticum urartu, Aegilops tauschii, and Triticum aestivum during the origin of bread wheat. Gene ontology analysis revealed that PAL genes play a role in plant growth. We also identified 27 putative miRNAs targeting 37 TaPAL genes. The high expression level of PAL genes was detected in roots of drought-tolerant genotypes compared to drought-sensitive genotypes. However, very low expressions of TaPAL10, TaPAL30, TaPAL32, TaPAL3, and TaPAL28 were recorded in all wheat genotypes. Arogenate dehydratase interacts with TaPAL29 and has higher expression in roots. The analysis of all identified genes in RNA-seq data showed that they are expressed in roots and shoots under normal and abiotic stress. Our study offers valuable data on the functioning of PAL genes in wheat.

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The fungicide propamocarb increases lignin by activating the phenylpropanoid pathway in Cucumis sativus L.

Cited by 18 publications

References 29 publications

Diversity of Bacterial Microbiota of Coastal Halophyte Limonium sinense and Amelioration of Salinity Stress Damage by Symbiotic Plant Growth-Promoting Actinobacterium Glutamicibacter halophytocola KLBMP 5180

Diversity of Bacterial Microbiota of Coastal Halophyte Limonium sinense and Amelioration of Salinity Stress Damage by Symbiotic Plant Growth-Promoting Actinobacterium Glutamicibacter halophytocola KLBMP 5180

Expression and functional analysis of the Propamocarb-related gene CsDIR16 in cucumbers

Phenylalanine Ammonia-Lyase (PAL) Genes Family in Wheat (Triticum aestivum L.): Genome-Wide Characterization and Expression Profiling

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