The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

Mäkelä, Miia; Pontes, María Victoria Aguilar; Rossen-Uffink, Diana van; Peng, Mao; Vries, Ronald P. de

doi:10.1038/s41598-018-25152-x

Cited by 40 publications

(16 citation statements)

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“…However, transport processes (GO:0006810) and metabolic processes do not seem to be synchronized. This is in line with the results of a previous study (Mäkelä et al, 2018), where sugar transport and metabolism were shown not to be co-regulated during growth of A. niger in liquid cultures. Interestingly, after 8 h of growth on SBP, an overall repression of genes involved in most of the studied GO terms was observed for all three mutants.…”

Section: The Different a Niger Pcp Deletion Mutants Cause Significansupporting

confidence: 93%

“…The expression of the RCP genes was significantly upregulated in the Δ larA Δ xyrA Δ xyrB mutant, compared to the reference strain after 24 h of growth on SBP. The delayed response of the RCP genes on SBP, compared to the previously shown PCP and GACP genes, could be simply attributed to the sequential manner in which A. niger consumes sugars in liquid cultures ( Mäkelä et al, 2018 ). L -rhamnose has been shown to be the least preferred carbon source between them, and as a result, the upregulation of the genes involved in its catabolism was significantly delayed compared to the other sugars ( Figure 3F ).…”

Section: Discussionmentioning

confidence: 97%

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Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

Chroumpi

Peng

Markillie

et al. 2021

Front. Bioeng. Biotechnol.

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The filamentous ascomycete Aspergillus niger has received increasing interest as a cell factory, being able to efficiently degrade plant cell wall polysaccharides as well as having an extensive metabolism to convert the released monosaccharides into value added compounds. The pentoses D-xylose and L-arabinose are the most abundant monosaccharides in plant biomass after the hexose D-glucose, being major constituents of xylan, pectin and xyloglucan. In this study, the influence of selected pentose catabolic pathway (PCP) deletion strains on growth on plant biomass and re-routing of sugar catabolism was addressed to gain a better understanding of the flexibility of this fungus in using plant biomass-derived monomers. The transcriptome, metabolome and proteome response of three PCP mutant strains, ΔlarAΔxyrAΔxyrB, ΔladAΔxdhAΔsdhA and ΔxkiA, grown on wheat bran (WB) and sugar beet pulp (SBP), was evaluated. Our results showed that despite the absolute impact of these PCP mutations on pure pentose sugars, they are not as critical for growth of A. niger on more complex biomass substrates, such as WB and SBP. However, significant phenotypic variation was observed between the two biomass substrates, but also between the different PCP mutants. This shows that the high sugar heterogeneity of these substrates in combination with the high complexity and adaptability of the fungal sugar metabolism allow for activation of alternative strategies to support growth.

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Section: The Different a Niger Pcp Deletion Mutants Cause Significansupporting

confidence: 93%

Section: Discussionmentioning

confidence: 97%

Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

Chroumpi

Peng

Markillie

et al. 2021

Front. Bioeng. Biotechnol.

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“…Glucose appeared to repress mucic acid production in D-161646, whereas production by the marine transformants was similar with d-glucose or glycerol as co-substrate (Table 1). It has been shown that uptake of d-galacturonic acid can be repressed by the presence of other substrates [32,33]. Co-substrates, d-xylose, glycerol, d-glucose or lactose, were generally consumed by Trichoderma strains before sampling started at 72 h, which should also have relieved initial repression effects.…”

Section: Discussionmentioning

confidence: 99%

Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

et al. 2020

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Background: Two marine fungi, a Trichoderma sp. and a Coniochaeta sp., which can grow on d-galacturonic acid and pectin, were selected as hosts to engineer for mucic acid production, assessing the suitability of marine fungi for production of platform chemicals. The pathway for biotechnologcial production of mucic (galactaric) acid from d-galacturonic acid is simple and requires minimal modification of the genome, optimally one deletion and one insertion. d-Galacturonic acid, the main component of pectin, is a potential substrate for bioconversion, since pectin-rich waste is abundant. Results: Trichoderma sp. LF328 and Coniochaeta sp. MF729 were engineered using CRISPR-Cas9 to oxidize d-galacturonic acid to mucic acid, disrupting the endogenous pathway for d-galacturonic acid catabolism when inserting a gene encoding bacterial uronate dehydrogenase. The uronate dehydrogenase was expressed under control of a synthetic expression system, which fucntioned in both marine strains. The marine Trichoderma transformants produced 25 g L −1 mucic acid from d-galacturonic acid in equimolar amounts: the yield was 1.0 to 1.1 g mucic acid [g d-galacturonic acid utilized] −1. d-Xylose and lactose were the preferred co-substrates. The engineered marine Trichoderma sp. was more productive than the best Trichoderma reesei strain (D-161646) described in the literature to date, that had been engineered to produce mucic acid. With marine Coniochaeta transformants, d-glucose was the preferred cosubstrate, but the highest yield was 0.82 g g −1 : a portion of d-galacturonic acid was still metabolized. Coniochaeta sp. transformants produced adequate pectinases to produce mucic acid from pectin, but Trichoderma sp. transformants did not. Conclusions: Both marine species were successfully engineered using CRISPR-Cas9 and the synthetic expression system was functional in both species. Although Coniochaeta sp. transformants produced mucic acid directly from pectin, the metabolism of d-galacturonic acid was not completely disrupted and mucic acid amounts were low. The d-galacturonic pathway was completely disrupted in the transformants of the marine Trichoderma sp., which produced more mucic acid than a previously constructed T. reesei mucic acid producing strain, when grown under similar conditions. This demonstrated that marine fungi may be useful as production organisms, not only for native enzymes or bioactive compounds, but also for other compounds.

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“…The fungus A. niger can utilize biomass polysaccharides (hemicellulose) as carbon source for fungal growth by degrading these compounds into monomeric sugars. Furthermore, these monomeric sugars were transported into the cells and by used catabolic pathway to convert them into biochemical building blocks and energy (Makela et al 2018). The fungus A. niger can use these building blocks and energy for fungal growth and increased β-glucosidase production.…”

Section: Effect Of Different Carbon Sources On Enzyme Productionmentioning

confidence: 99%

Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate

et al. 2020

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Abstract. Sugiwati S, Hanafi M, Lioe HN, Suhartono MT. 2020. Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate. Biodiversitas 21: 4058-4066. β-Glucosidase is the family of glycosyl hydrolase that have potential role in various food industry, such as in tea, wine and vanilla industries to increase the aroma and production of isoflavone aglycons in soybean flour. The present work produced β-glucosidase from local isolate of Aspergillus niger InaCC F57 under solid-state fermentation (SSF) using rice bran substrate. Fermentation process was made in various conditions with respect to carbon source as substrate, initial pH of fermentation medium, incubation time, water to substrate ratio, fermentation temperature, and addition of Mandels mineral salts solution. The results showed that activity of β-glucosidase was best at, i.e., 2.45 U/mL, with the use of rice bran as substrate. Furthermore, optimum condition for the highest production of β-glucosidase occurred at pH 2.0, incubation time of 5 days, water to substrate ratio of 1.5: 1, and incubation temperature of 32°C. Additionally, in optimum fermentation conditions, production of β-glucosidase could be enhanced up to 26.22% with the presence of Mandels mineral salts solution as compared to the control.

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The fungus Aspergillus niger consumes sugars in a sequential manner that is not mediated by the carbon catabolite repressor CreA

Cited by 40 publications

References 41 publications

Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

Re-routing of Sugar Catabolism Provides a Better Insight Into Fungal Flexibility in Using Plant Biomass-Derived Monomers as Substrates

Engineering marine fungi for conversion of d-galacturonic acid to mucic acid

Effect of growth conditions on β-glucosidase production by local isolate of Aspergillus niger using rice bran substrate

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