The Future of DNA Barcoding: Reflections from Early Career Researchers

Grant, Danielle; Brodnicke, Ole Bjørn; Evankow, Ann; Ferreira, André O.; Fontes, João T.; Hansen, Anker; Jensen, Mads Radmer; Kalayci, Tuğba Ergül; Leeper, Alexandra; Patil, Shalaka Kiran; Prati, Sebastian; Reunamo, Anna; Roberts, Aradhana J.; Shigdel, Rajesh; Tyukosova, Valentina; Bendiksby, Mika; Blaalid, Rakel; Costa, Filipe O.; Hollingsworth, Peter; Stur, Elisabeth; Ekrem, Torbjørn

doi:10.3390/d13070313

Cited by 41 publications

(23 citation statements)

References 67 publications

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“…Curation of barcoding data, as we have done here, is a critical step for using DNA barcodes in the future [54]. This paper demonstrates the use of DNA barcoding beyond simply biodiversity and biomonitoring analysis.…”

Section: Conclusion and Future Considerationsmentioning

confidence: 78%

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

et al. 2022

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Chironomids are one of the most biodiverse and abundant members of freshwater ecosystems. They are a food source for many organisms, including fish and water mites. The accurate identification of chironomids is essential for many applications in ecological research, including determining which chironomid species are present in the diets of diverse predators. Larval and adult chironomids from diverse habitats, including lakes, rivers, inland gardens, coastal vegetation, and nearshore habitats of the Great Lakes, were collected from 2012 to 2019. After morphological identification of chironomids, DNA was extracted and cytochrome oxidase I (COI) barcodes were PCR amplified and sequenced. Here we describe an analysis of biodiverse adult and larval chironomids in the Great Lakes region of North America based on new collections to improve chironomid identification by curating a chironomid DNA barcode database, thereby expanding the diversity and taxonomic specificity of DNA reference libraries for the Chironomidae family. In addition to reporting many novel chironomid DNA barcodes, we demonstrate here the use of this chironomid COI barcode database to improve the identification of DNA barcodes of prey in the liquefied diets of water mites. The species identifications of the COI barcodes of chironomids ingested by Lebertia davidcooki and L. quinquemaculosa are more diverse for L. davidcooki and include Parachironomus abortivus, Cryptochironomus ponderosus. Parachironomus tenuicaudatus, Glyptotendipes senilis, Dicrotendipes modestus, Chironomus riparius, Chironomus entis/plumosus, Chironomus maturus, Chironomus crassicaudatus, Endochironomus subtendens, Cricotopus sylvestris, Cricotopus festivellus, Orthocladius obumbratus, Tanypus punctipennis, Rheotanytarsus exiguus gr., and Paratanytarsus nr. bituberculatus.

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Section: Conclusion and Future Considerationsmentioning

confidence: 78%

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

et al. 2022

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“…Recent reviews have highlighted the growth of DNA barcode applications for phylogenetics and taxonomy (e.g., [2]). Other overviews suggest that DNA barcoding is a resilient field that will continue to grow as sequence databases are enriched, throughput expands, and automation provides an ever-expanding user-community with increased accessibility to DNA barcodes, as reported by [3]. This review highlights the advances and applications in DNA barcode sequencing that have been leveraged for novel research in ecology, evolution, and conservation.…”

Section: Introductionmentioning

confidence: 96%

The Expanding Role of DNA Barcodes: Indispensable Tools for Ecology, Evolution, and Conservation

Gostel

Kress

2022

Diversity

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DNA barcoding has transformed the fields of ecology, evolution, and conservation by providing a rapid and effective tool for species identification. The growth of DNA barcodes as a resource for biologists has followed advances in computational and sequencing technology that have enabled high-throughput barcoding applications. The global DNA barcode database is expanding to represent the diversity of species on Earth thanks to efforts by international consortia and expanding biological collections. Today, DNA barcoding is instrumental in advancing our understanding of how species evolve, how they interact, and how we can slow down their extirpation and extinction. This review focuses on current applications of DNA barcode sequences to address fundamental lines of research, as well as new and expanding applications of which DNA barcoding will play a central role.

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“…Plant identification using micro-morphological, chemical and organoleptic methods can be time-consuming, error-prone and requires expertise and reliable references [ 51 , 52 , 53 ]. In comparison, DNA barcoding is more reliable, is not affected by external factors and can be applied to all tissues [ 54 , 55 ]. Over the past two decades, this approach has been increasingly accepted for the identification of plants.…”

Section: Introductionmentioning

confidence: 99%

“…DNA barcoding provides a way to confirm the authentication of plants and establish a level of quality assurance within the market [ 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 ]. Since the first DNA barcoding study [ 60 ], the “animal barcode”, a portion of the mitochondrial gene cytochrome oxidase 1( CO 1), has proved remarkably effective at discriminating among species in diverse groups, such as birds, fishes, and insects [ 60 , 61 ].…”

Section: Introductionmentioning

confidence: 99%

Challenges in Medicinal and Aromatic Plants DNA Barcoding—Lessons from the Lamiaceae

Nazar

Howard

Slater

et al. 2022

Plants

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The potential value of DNA barcoding for the identification of medicinal plants and authentication of traded plant materials has been widely recognized; however, a number of challenges remain before DNA methods are fully accepted as an essential quality control method by industry and regulatory authorities. The successes and limitations of conventional DNA barcoding are considered in relation to important members of the Lamiaceae. The mint family (Lamiaceae) contains over one thousand species recorded as having a medicinal use, with many more exploited in food and cosmetics for their aromatic properties. The family is characterized by a diversity of secondary products, most notably the essential oils (EOs) produced in external glandular structures on the aerial parts of the plant that typify well-known plants of the basil (Ocimum), lavender (Lavandula), mint (Mentha), thyme (Thymus), sage (Salvia) and related genera. This complex, species-rich family includes widely cultivated commercial hybrids and endangered wild-harvested traditional medicines, and examples of potential toxic adulterants within the family are explored in detail. The opportunities provided by next generation sequencing technologies to whole plastome barcoding and nuclear genome sequencing are also discussed with relevant examples.

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The Future of DNA Barcoding: Reflections from Early Career Researchers

Cited by 41 publications

References 67 publications

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

Improved Chironomid Barcode Database Enhances Identification of Water Mite Dietary Content

The Expanding Role of DNA Barcodes: Indispensable Tools for Ecology, Evolution, and Conservation

Challenges in Medicinal and Aromatic Plants DNA Barcoding—Lessons from the Lamiaceae

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