The future of forensic DNA analysis

Butler, John M.

doi:10.1098/rstb.2014.0252

Cited by 177 publications

(117 citation statements)

References 64 publications

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“…STR typing continues to be the primary workhorse in forensic DNA analysis although other genetic markers could be used for specific applications . The United States launched a 13 core STR loci (TH01, vWA, FGA, D8S1179, D18S51, D21S11, CSF1PO, TPOX, D3S1358, D5S818, D7S820, D13S317, and D16S539) known as the Combined DNA Index System (CODIS) into its national database in October 1998 .…”

Section: Introductionmentioning

confidence: 99%

Development of a new 25plex STRs typing system for forensic application

et al. 2019

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We have developed a novel STR 25‐plex florescence multiplex‐STR kit (DNATyper25) to genotype 23 autosomal and two sex‐linked loci for forensic applications and paternity analysis. Of the 23 autosomal loci, 20 are non‐CODIS. The sex‐linked markers include a Y‐STR locus (DYS391) and the Amelogenin gene. We present developmental validation studies to show that the DNATyper25 kit is reproducible, accurate, sensitive, and robust. Sensitivity testing showed that full profiles were achieved with as low as 125 pg of human DNA. Specificity testing demonstrated a lack of cross reactivity with a variety of commonly encountered non‐human DNA contaminants. Stability testing showed that full profiles were obtained with humic acid concentration ≤60 ng/μL and hematin concentration <400 μM. For forensic evaluation, the 23 autosomal STRs followed the Hardy–Weinberg equilibrium. In an analysis of 509 Chinese (CN) Hans, we detected a combined total of 181 alleles at the 23 autosomal STR loci. Since these autosomal STRs are independent from one another, PM was 8.4528 × 10−22, TDP was 0.999 999 999 999 999 999 999, CEP was 0.999 999 8395. The forensic efficiency parameters demonstrated that these autosomal STRs are highly polymorphic and informative in the Han population of China. We performed population comparisons and showed that the Northern CN Han has a close genetic relationship with the Luzhou Han, Tujia, and Bai populations. We propose that the DNATyper25 kit will be useful for cases where paternity analysis is difficult and for situations where DNA samples are limited in quantity and low in quality.

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Section: Introductionmentioning

confidence: 99%

Development of a new 25plex STRs typing system for forensic application

et al. 2019

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“…Though novel training [4] and funding initiatives [5] have helped to contend with the demand, forensic biology has proven to be indispensable for suspect identification, leading to a significant level of outsourcing to private labs [6,7]. While the development of higher performance analytical methods is seen as a means of accommodating the increasing number of these requests [8,9], so too are techniques that reduce the impact of other types of forensic evidence [5,10], allowing the reallocation of resources towards the more laborious biological analyses.…”

Section: A Review Of the 2009 Census For Publicly Funded Forensicmentioning

confidence: 99%

Analytical Validation of a Portable Mass Spectrometer Featuring Interchangeable, Ambient Ionization Sources for High Throughput Forensic Evidence Screening

Lawton

Traub

Fatigante

et al. 2016

J. Am. Soc. Mass Spectrom.

103

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Abstract. Forensic evidentiary backlogs are indicative of the growing need for costeffective, high-throughput instrumental methods. One such emerging technology that shows high promise in meeting this demand while also allowing on-site forensic investigation is portable mass spectrometric (MS) instrumentation, particularly that which enables the coupling to ambient ionization techniques. While the benefits of rapid, on-site screening of contraband can be anticipated, the inherent legal implications of field-collected data necessitates that the analytical performance of technology employed be commensurate with accepted techniques. To this end, comprehensive analytical validation studies are required before broad incorporation by forensic practitioners can be considered, and are the focus of this work. Pertinent performance characteristics such as throughput, selectivity, accuracy/precision, method robustness, and ruggedness have been investigated. Reliability in the form of false positive/negative response rates is also assessed, examining the effect of variables such as user training and experience level. To provide flexibility toward broad chemical evidence analysis, a suite of rapidly-interchangeable ion sources has been developed and characterized through the analysis of common illicit chemicals and emerging threats like substituted phenethylamines.

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“…KEYWORDS: forensic science, forensic genetics, next-generation sequencing, short tandem repeat, Ion S5 TM XL, Ion Chef TM Short tandem repeat (STR) genotyping using capillary electrophoresis (CE) platforms has been the dominant technology in forensic DNA laboratories (1). As the gold standard for individual identification, CE is simple, economic, fast, and accepted by courts.…”

mentioning

confidence: 99%

Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems,

Wang

Chen

et al. 2018

Journal of Forensic Sciences

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Next-generation sequencing (NGS) has been used to genotype forensic short tandem repeat (STR) markers for individual identification and kinship analysis. STR data from several NGS platforms have been published, but forensic application trials using the Ion S5™ XL system have not been reported. In this work, we report sensitivity, reproducibility, mixture, simulated degradation, and casework sample data on the Ion Chef™ and S5™ XL systems using an early access 25-plex panel. Sensitivity experiments showed that over 97% of the alleles were detectable with down to 62 pg input of genomic DNA. In mixture studies, alleles from minor contributors were correctly assigned at 1:9 and 9:1 ratios. NGS successfully gave 12 full genotype results from 13 challenging casework samples, compared with five full results using the CE platform. In conclusion, the Ion Chef™ and the Ion S5™ XL systems provided an alternative and promising approach for forensic STR genotyping.

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The future of forensic DNA analysis

Cited by 177 publications

References 64 publications

Development of a new 25plex STRs typing system for forensic application

Development of a new 25plex STRs typing system for forensic application

Analytical Validation of a Portable Mass Spectrometer Featuring Interchangeable, Ambient Ionization Sources for High Throughput Forensic Evidence Screening

Massively Parallel Sequencing of Forensic STRs Using the Ion Chef™ and the Ion S5™ XL Systems,

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