The FvemiR167b-FveARF6 module increases the number of roots and leaves in woodland strawberry

FvemiR160‐FveARF18A‐FveAP1/FveFUL module regulates flowering time in woodland strawberry

“…However, there are relatively few reports on the regulation of flowering by IAA in plants, and studies have shown that IAA can induce flower bud differentiation in Arabidopsis and tobacco (Shimazu et al, 2000;Smulders et al, 1988), but the mechanism by which IAA regulates flowering is unclear. Our previous research found that FvemiR390 induced by IAA promotes the synthesis of FvetasiRNA3, and FvetasiRNA3 can negatively regulate its expression by targeting FveARF4, and then FveARF4 regulates the flowering of woodland strawberries by activating the expression of FveAP1/Fve-FUL (Dong et al, 2022;Dong, Li, et al, 2021). In summary, this study revealed the mechanism by which IAA regulates strawberry flowering via the miR390-tasiRNA3-ARF4 module.…”

“…According to Li, Wang, et al. 's method (2022), the RLM‐5′ RACE assay was performed to verify FveARF18A is the target gene of FvemiR160a. All primers involved in this study are listed in Table S5.…”

“…According to Li, Wang, et al. 's method (2022), the expression of the FvemiR160a, FvemiR390, FveARF4 (FvH4_3g32860), FveARF17 (FvH4_5g38770), FveARF18A / mFveARF18A (FvH4_3g32000), FveARF18B (FvH4_6g15960), FveARF18C (FvH4_7g19350), FveAP1 (FvH4_4g29600), FveFUL (FvH4_5g13500), FveSOC1 (FvH4_7g127000), FveLFY (FvH4_5g09660), FveFT (FvH4_6g00090), and FveTFL1 (FvH4_6g18480) was detected. We calculated the expression of genes using the 2 −ΔΔCt method, and the Fve26S gene of strawberry serves as the internal control gene.…”

“…In addition, the flower organs of ornamental plants are the main sites for measuring their economic value; therefore, their economic value can be improved by adjusting flowering time (Cardoso et al, 2016). The strawberry is an important berry that is widely cultivated worldwide and has significant nutritional and economic value (Dong et al, 2022). During production, early flowering means the early ripening of strawberry fruits, allowing strawberries to be harvested and marketed in advance, greatly improving their economic benefits (Koskela et al, 2012;Liu et al, 2017).…”

“…In woodland strawberries, FvemiR164a participates in floral organ formation and leaf development (Zheng et al., 2019). Additionally, our previous results demonstrated that FveAP1 / FveFUL as the target genes of FveARF4 are regulated by the miR390‐tasiRNA3 module in the flowering of ‘Ruegen’ strawberry (Dong et al., 2022; Dong, Li, et al., 2021); overexpression of FvemiR167b in ‘Ruegen’ increases the number of leaves and roots by targeting FveARF6 (Li, Wang, et al., 2022). Furthermore, the leaf phosphorus and fruit‐soluble solids of FvemiR399a overexpression strawberry plants were significantly increased (Wang, Zhang, et al., 2019).…”