The FvPHR1 transcription factor control phosphate homeostasis by transcriptionally regulating miR399a in woodland strawberry

Wang, Yan; Zhang, Feng; Cui, Weixu; Chen, Keqin; Zhao, Rui

doi:10.1016/j.plantsci.2018.12.025

Cited by 26 publications

(17 citation statements)

References 56 publications

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“…Comparison of Figure 1B to Figure 1A, and Figure 1E to Figure 1D, clearly shows that the constitutive expression of the miR399-specific eTM transgene in the selected MIM399 transformant line confirmed this previous report [41] with MIM399 plants phenotypically indistinguishable to Col-0 plants throughout the vegetative and reproductive phases of development. In pummelo (Citrus grandis) plants however, the expression of a miR399-specific eTM transgene resulted in the expression of readily observable phenotypic defects in the floral tissues, including smaller sized petals and sepals, reduced petal numbers, short anther filaments and reduced anther numbers [42]. However, the Arabidopsis MIM399 plants generated in this study did not display any readily observable differences to Col-0 plants during their reproductive development.…”

Section: Generation Of Arabidopsis Transformant Lines Molecularly Modmentioning

confidence: 68%

Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Pegler

Oultram

Grof

et al. 2020

Plants

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In Arabidopsis thaliana (Arabidopsis), the microRNA399 (miR399)/PHOSPHATE2 (PHO2) expression module is central to the response of Arabidopsis to phosphate (PO4) stress. In addition, miR399 has been demonstrated to also alter in abundance in response to salt stress. We therefore used a molecular modification approach to alter miR399 abundance to investigate the requirement of altered miR399 abundance in Arabidopsis in response to salt stress. The generated transformant lines, MIM399 and MIR399 plants, with reduced and elevated miR399 abundance respectively, displayed differences in their phenotypic and physiological response to those of wild-type Arabidopsis (Col-0) plants following exposure to a 7-day period of salt stress. However, at the molecular level, elevated miR399 abundance, and therefore, altered PHO2 target gene expression in salt-stressed Col-0, MIM399 and MIR399 plants, resulted in significant changes to the expression level of the two PO4 transporter genes, PHOSPHATE TRANSPORTER1;4 (PHT1;4) and PHT1;9. Elevated PHT1;4 and PHT1;9 PO4 transporter levels in salt stressed Arabidopsis would enhance PO4 translocation from the root to the shoot tissue which would supply additional levels of this precious cellular resource that could be utilized by the aerial tissues of salt stressed Arabidopsis to either maintain essential biological processes or to mount an adaptive response to salt stress.

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Section: Generation Of Arabidopsis Transformant Lines Molecularly Modmentioning

confidence: 68%

Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Pegler

Oultram

Grof

et al. 2020

Plants

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“…Phosphate starvation also induces miRNA responses in Populus tomentosa [6]. Moreover, as a longdistance signal, miR399 can regulate phosphate homeostasis in plants [7] and can serve as a potential integrator of photoresponses and phosphate homeostasis [8,9]. In Arabidopsis, a new class of small RNAs has been identified in phosphate-starved roots [10], and these small RNA mediate responses to P deficiency and regulate P homeostasis [11].…”

mentioning

confidence: 99%

Transcriptome-wide identification of novel circular RNAs in soybean in response to low-phosphorus stress

Lü

et al. 2020

PLoS ONE

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Low-phosphorus (LP) stress is a major factor limiting the growth and yield of soybean. Circular RNAs (circRNAs) are novel noncoding RNAs that play a crucial role in plant responses to abiotic stress. However, how LP stress mediates the biogenesis of circRNAs in soybean remains unclear. Here, to explore the response mechanisms of circRNAs to LP stress, the roots of two representative soybean genotypes with different P-use efficiency, Bogao (a LPsensitive genotype) and Nannong 94156 (a LP-tolerant genotype), were used for the construction of RNA sequencing (RNA-seq) libraries and circRNA identification. In total, 371 novel circRNA candidates, including 120 significantly differentially expressed (DE) cir-cRNAs, were identified across different P levels and genotypes. More DE circRNAs were significantly regulated by LP stress in Bogao than in NN94156, suggesting that the tolerant genotype was less affected by LP stress than the sensitive genotype was; in other words, NN94156 may have a better ability to maintain P homeostasis under LP stress. Moreover, a positive correlation was observed between the expression patterns of P stress-induced cir-cRNAs and their circRNA-host genes. Gene Ontology (GO) enrichment analysis of these circRNA-host genes and microRNA (miRNA)-targeted genes indicated that these DE cir-cRNAs were involved mainly in defense responses, ADP binding, nucleoside binding, organic substance catabolic processes, oxidoreductase activity, and signal transduction. Together, our results revealed that LP stress can significantly alter the genome-wide profiles of circRNAs and indicated that the regulation of circRNAs was both genotype and environment specific in response to LP stress. LP-induced circRNAs might provide a rich resource for LP-responsive circRNA candidates for future studies.

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“…Long‐distance movement of miR399 was found to enhance Pi uptake and translocation via regulation of PHT1 during the onset of Pi deficiency (Lin et al, ). Furthermore, Y. Wang et al () observed that the strawberry ( Fragaria vesca ) MYB protein FvPHR1 directly binds to the FvmiR399a promoter and induces the expression of FvmiR399a . In addition, overexpression of FvPHR1 in strawberry resulted in a significant increase in the Pi concentration in leaves (Y. Wang et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, Y. Wang et al () observed that the strawberry ( Fragaria vesca ) MYB protein FvPHR1 directly binds to the FvmiR399a promoter and induces the expression of FvmiR399a . In addition, overexpression of FvPHR1 in strawberry resulted in a significant increase in the Pi concentration in leaves (Y. Wang et al, ). In Arabidopsis, AtMYB2 directly binds to an MYB‐binding site in the promoter of the miR399f precursor and regulates miR399f expression during Pi starvation, influencing salt and drought stress responses (Baek et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…In Arabidopsis, AtMYB2 directly binds to an MYB‐binding site in the promoter of the miR399f precursor and regulates miR399f expression during Pi starvation, influencing salt and drought stress responses (Baek et al, ). These studies suggest that MYB proteins are involved in Pi uptake and translocation as well as in stress responses mediated by miR399 and PHT1;4 during Pi deficiency (Baek et al, ; Y. Wang et al, ). Pi deficiency may induce anthocyanin accumulation via regulation of the expression of structural genes in the flavonoid pathway by miR399d and PHT1;4 .…”

Section: Discussionmentioning

confidence: 99%

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MiR399d and epigenetic modification comodulate anthocyanin accumulation in Malus leaves suffering from phosphorus deficiency

Peng

Tian

Luo

et al. 2020

Plant Cell & Environment

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Inorganic phosphorus (Pi) deficiency induces anthocyanin accumulation in the leaves of some plant species; however, the molecular mechanisms underlying this phenomenon have not been well characterized. Here, we showed that microRNA399d (miR399d), high-affinity Pi transporter McPHT1;4, and McMYB10 are strongly induced in Malus leaves suffering from Pi deficiency. By culturing explants of transiently transformed plants in MS medium under conditions of Pi sufficiency and Pi deficiency, miR399d and McPHT1;4 were shown to play essential roles in the response to Pi deficiency and to play positive roles in the regulation of anthocyanin biosynthesis. Silencing of McHDA6 expression and treatment with the inhibitor trichostatin A suggested that the low expression of McHDA6 simultaneously reduced the transcription of McMET1 and decreased the methylation level of the McMYB10 promoter; however, the expression of McMYB10 and anthocyanin content were increased. Bimolecular fluorescence complementation and yeast two-hybrid assays revealed that McHDA6 binds directly to McMET1 through its BAH2 and DNMT1-RFD domains. Based on the results of our study, we propose a mechanism for the molecular regulation of anthocyanin biosynthesis, namely, the miR399d and epigenetic modification comodulation model, to explain the phenomenon in which leaves turn red under conditions of Pi deficiency. K E Y W O R D S anthocyanin accumulation, epigenetic modification, Malus crabapple, McMYB10, miR399d, phosphorus deficiency

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The FvPHR1 transcription factor control phosphate homeostasis by transcriptionally regulating miR399a in woodland strawberry

Cited by 26 publications

References 56 publications

Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Molecular Manipulation of the miR399/PHO2 Expression Module Alters the Salt Stress Response of Arabidopsis thaliana

Transcriptome-wide identification of novel circular RNAs in soybean in response to low-phosphorus stress

MiR399d and epigenetic modification comodulate anthocyanin accumulation in Malus leaves suffering from phosphorus deficiency

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