The galactose biosensor based on microporous polyacrylonitrile

Kan, Jinqing; Chen, Cheng; Jing, Guolin

doi:10.1080/10242420500389553

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…For instance, the band at 1027 cm −1 was related to the isoindole deformation and aza stretching of CuPc S , and the band at 1174 cm −1 was related to S=O symmetric stretching vibrations in sulfonic groups [ 41 ]. Characteristic bands of CHI appeared at 3400 cm −1 , corresponding to the OH stretching vibrations, and 1367 cm −1 , due to the C–H–OH bending and CH 2 OH stretching, respectively [ 42 ]. The main bands produced by the IL appeared at 1647 cm −1 , corresponding to the C=C and C=N bonds (C–N stretching), and at 1078 cm −1 , related to C–H in-plane deformation [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Discrimination of Milks with a Multisensor System Based on Layer-by-Layer Films

Salvo-Comino

García-Hernández

Garcı́a

et al. 2018

Sensors

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A nanostructured electrochemical bi-sensor system for the analysis of milks has been developed using the layer-by-layer technique. The non-enzymatic sensor [CHI+IL/CuPcS]2, is a layered material containing a negative film of the anionic sulfonated copper phthalocyanine (CuPcS) acting as electrocatalytic material, and a cationic layer containing a mixture of an ionic liquid (IL) (1-butyl-3-methylimidazolium tetrafluoroborate) that enhances the conductivity, and chitosan (CHI), that facilitates the enzyme immobilization. The biosensor ([CHI+IL/CuPcS]2-GAO) results from the immobilization of galactose oxidase on the top of the LbL layers. FTIR, UV–vis, and AFM have confirmed the proposed structure and cyclic voltammetry has demonstrated the amplification caused by the combination of materials in the film. Sensors have been combined to form an electronic tongue for milk analysis. Principal component analysis has revealed the ability of the sensor system to discriminate between milk samples with different lactose content. Using a PLS-1 calibration models, correlations have been found between the voltammetric signals and chemical parameters measured by classical methods. PLS-1 models provide excellent correlations with lactose content. Additional information about other components, such as fats, proteins, and acidity, can also be obtained. The method developed is simple, and the short response time permits its use in assaying milk samples online.

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Section: Resultsmentioning

confidence: 99%

Discrimination of Milks with a Multisensor System Based on Layer-by-Layer Films

Salvo-Comino

García-Hernández

Garcı́a

et al. 2018

Sensors

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“…In another study [ 18 ], GalOx was physically adsorbed onto microporous polyacrylonitrile thin films. The biosensor appeared to have good stability and was highly reproducible.…”

Section: Galactose Oxidase-based Biosensorsmentioning

confidence: 99%

Enzyme-based amperometric galactose biosensors: a review

et al. 2017

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This review (with 35 references) summarizes the various strategies used in biosensors for galactose, and their analytical performance. A brief comparison of the enzyme immobilization methods employed and the analytical performance characteristics of a range of galactose biosensors are first summarized in tabular form and then described in detail. Selected examples have been included to demonstrate the various applications of these biosensors to real samples. Following an introduction into the field that covers the significance of sensing galactose in various fields, the review covers biosensors based on the use of galactose oxidase, with a discussion of methods for their immobilization (via cross-linking, adsorption, covalent bonding and entrapment). This is followed by a short section on biosensors based on the use of galactose dehydrogenase. The conclusion section summarizes the state of the art and addresses current challenges. Graphical abstractFabrication of a disposable screen-printed (a) electrochemical galactose biosensor (b) for real sample analysis and a dummy biosensor (c) for compensating the effect of interferences

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“…For instance, the band at 1027 cm -1 was related to the isoindole deformation and aza stretching of CuPc S and the band at 1174 cm -1 was related to S=O symmetric stretching vibrations in sulfonic groups [36]. Characteristic bands of CHI appeared at 3400 cm -1 corresponding to the OH stretching vibrations, and 1367 cm −1 due to the C-H-OH bending and CH2OH stretching respectively [37]. The main bands produced by the IL appeared at 1647 cm -1 corresponding to the C=C and C=N bonds (C-N stretching) and at 1078 cm -1 related to C-H in-plane deformation [38].…”

Section: Structural Characterization Of the Sensorsmentioning

confidence: 99%

Multisensor System Based on Layer by Layer Films. Discrimination of Milks

Salvo-Comino¹,

García-Hernández²,

Garcı́a³

et al. 2018

Preprint

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Abstract:A nanostructured electrochemical bi-sensor system for analysis of milks has been developed using the Layer by Layer technique. The non-enzymatic sensor [CHI+IL/CuPc S ]2, is a layered material containing a negative film of the anionic sulfonated copper phthalocyanine (CuPc S ) acting as electrocatalytic material, and a cationic layer containing a mixture of an ionic liquid (IL) (1-butyl-3-methylimidazolium tetrafluoroborate) that enhances the conductivity and chitosan (CHI) that facilitates the enzyme immobilization. The biosensor ([CHI+IL/CuPc S ]2-GAO) results from the immobilization of galactose oxidase on the top of the LbL layers. FTIR, UV-vis and AFM have confirmed the proposed structure and cyclic voltammetry has demonstrated the amplification caused by the combination of materials in the film. Sensors have been combined to form an electronic tongue for milk analysis. Principal Component Analysis has revealed the ability of the sensor system to discriminate between milk samples with different lactose content. Using PLS-1 calibration models, correlations have been found between the voltammetric signals and chemical parameters measured by classical methods. PLS-1 models provide excellent correlations with lactose content. Additional information about other components such as fats, proteins and acidity can also be obtained. The method developed is simple and the short response time permits its use in assaying milk samples on-line.

show abstract

The galactose biosensor based on microporous polyacrylonitrile

Cited by 8 publications

References 22 publications

Discrimination of Milks with a Multisensor System Based on Layer-by-Layer Films

Discrimination of Milks with a Multisensor System Based on Layer-by-Layer Films

Enzyme-based amperometric galactose biosensors: a review

Multisensor System Based on Layer by Layer Films. Discrimination of Milks

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