Bivalve molluscs are an important food for most coastal inhabitants in temperate and tropical areas (Chávez-Villalba et al., 2024). Pinctada margaritifera Linnaeus, 1758 (Bivalvia: Pteriidae) is known as the black-lipped pearl oyster (Kishore & Southgate, 2016). This species exhibits a wide-ranging distribution in the Indo-Pacific zone, such as the Red Sea, Japan, Madagascar, Australia, and Hawaii. It also distributes on the tropical Pacific shoreline of the Americas, identified as P. mazatlanica as a synonym (Tëmkin, 2010). P. margaritifera, is used to produce a type of pearl known as Tahitian cultured pearls (El-Refi et al., 2022). P. margaritifera exhibits protandric hermaphroditism, a reproductive strategy in which it undergoes a transition from the male to the female sex during its growth and development. The presence of female individuals becomes