The Gaseous Environment of the Mammalian Cell in Culture

McLimans, William F.

doi:10.1016/b978-0-12-598301-3.50011-8

Cited by 38 publications

(16 citation statements)

References 124 publications

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“…In this paper, we describe an autoclavable fluorescence lifetime-based sensing film capable of on-line fermentation monitoring of dissolved CO 2 . The monitoring of carbon dioxide is important in bioprocess development because its elevated presence or absence has been shown to affect cell growth and product formation in prokaryotic and eukaryotic cells alike (10)(11)(12)(13)(14). Recently, Uttamlal and Walt (9) have first reported a steam-sterilizable fiberoptic CO 2 sensor for on-line fermentation monitoring, which is based on entrapped fluorophore-buffer solution in a Gore-Tex membrane.…”

Section: Introductionmentioning

confidence: 99%

Steam‐Sterilizable, Fluorescence Lifetime‐Based Sensing Film for Dissolved Carbon Dioxide

Chang

Randers‐Eichhorn

Lakowicz

et al. 1998

Biotechnology Progress

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An autoclavable sensing film was developed for monitoring dissolved CO2. The sensing film, based on fluorescence resonance energy transfer (FRET), consisted of a fluorescent donor, an acceptor, and a quaternary ammonium hydroxide, which were doped in a two-component silicone film. As no aqueous solution was used in the sensing film matrix, the sensing film was unaffected by osmotic pressure. Fluorescence lifetime was selected as the sensing parameter, and measured in frequency domain using phase fluorometry. Upon exposure to 20% CO2-saturated water, a 43 degrees increase in phase angle was observed at 100 MHz. The process was fully reversible when the sensing film was exposed to nitrogen-saturated water. The estimated response and recovery times for 90% signal change were 1 min (for a step change from 0 to 6.7% CO2-saturated water) and 1.5 min (for a step change from 6.7 to 3.3% CO2-saturated water). When used for on-line monitoring of dissolved CO2 produced by a culture of Escherichia coli, the sensing film showed a similar trend to that obtained from off-line measurements using a wet chemistry analyzer.

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Section: Introductionmentioning

confidence: 99%

Steam‐Sterilizable, Fluorescence Lifetime‐Based Sensing Film for Dissolved Carbon Dioxide

Chang

Randers‐Eichhorn

Lakowicz

et al. 1998

Biotechnology Progress

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“…on the in vitro growth and metabolic regul.ation of cells was reviewed by McLImans. 18 More extensive in vitro studies on adenoids for the elucidation of problems of differentiation in respiratory epithelia would require longer periods of culturing and improved quantitative characterization of new epithelial growths. For this purpose adenoid tissue represents some difficulties, such as the possibility of infection with adenoviruses and the association of the epithelium with lymphatic tissue.…”

Section: Discussionmentioning

confidence: 99%

Tissue Culture of Human Adult Adenoids and of Middle Ear Mucosa

Drucker¹,

Weisman²,

Sadé³

1976

Ann Otol Rhinol Laryngol

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The increased number of mucus producing cells as well as the presence of stratified squamous epithelium in pathological and experimental middle ear conditions, point towards the possibility of metaplastic changes of the middle ear mucosa, similar to the metaplastic capabilities of respiratory mucosae in general, as observed clinically or provoked experimentally. The purpose of this study was to develop a model of postembryonic human respiratory mucosae, in vitro, for the study of triggering or inducing factors involved in its normal and metaplastic differentiation. Explants from adenoids and middle ear mucosa were cultured, both as organ cultures and monolayers, for periods of up to two weeks, and their developmental characteristics were studied and described. Over 50% of the explants showed mitosis, epithelial and monolayer growth, ciliary activity and differentiation into ciliated and into mucus-producing cells. Adenoid explants were grown in air without and with added 5% CO 2 • Under the latter conditions, the proportion of explants and monolayers showing ciliary activity was 50% greater. It is concluded that this model might be suitable for further studies of the factors which control cyto-differentiation in mucociliary epithelia. Maintaining its growth for a longer period would, however, be desirable.

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“…However, the observed increase in relative plating efficiency was not greater than that found in cultures inoculated with 4 ml of amniotic fluid ( Table 3). The oxygen tension in the immediate environment of cells in culture is a function of cell density, metabolic activity, rate of diffusion of oxygen and oxygen tension in the culture (McLimans, 1972;Richter et a!., 1972;Bradley et al, 1978). Thus, the latter result was not unexpected, since amniotic fluid specimens from mid-trimester pregnancies display an extreme variability in the number of viable cells and the predominantly growing cell type (Hoehn et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

The effect of oxygen tension on colony formation and cell proliferation of amniotic fluid cellsin vitro

Held

Sönnichsen

1984

Prenatal Diagnosis

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The effects of reduced oxygen concentrations in the gas phase over the culture medium on colony formation and cell proliferation were investigated in high and low cell density primary and secondary cultures of amniotic fluid cells. Using two standard culture methods (25 cm2 plastic flasks and Leighton type tubes) a significantly reduced culture time was observed at high cell density for mass cultures by incubation within a low oxygen tension gas phase (2.5 per cent to 7.5 per cent O2) instead of conventional air (18 per cent O2). At low cell density colony formation was significantly enhanced in cultures grown at reduced oxygen tension. Using gas permeable membranes as support, lowering the oxygen tension from 7.5 per cent to 2.5 per cent yielded an increase in plating efficiency of cells from approximately 5 per cent to 25 per cent, whereas plating efficiency was less than 2 per cent for cells grown at ambient 18 per cent O2. It is suggested that low oxygen tension in the gas phase is an effective means of enhancing clonal growth in amniotic fluid cell cultures, thereby reducing both culture time and risk of culture failure.

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The Gaseous Environment of the Mammalian Cell in Culture

Cited by 38 publications

References 124 publications

Steam‐Sterilizable, Fluorescence Lifetime‐Based Sensing Film for Dissolved Carbon Dioxide

Steam‐Sterilizable, Fluorescence Lifetime‐Based Sensing Film for Dissolved Carbon Dioxide

Tissue Culture of Human Adult Adenoids and of Middle Ear Mucosa

The effect of oxygen tension on colony formation and cell proliferation of amniotic fluid cellsin vitro

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