The GATA-binding protein CGF-1 is closely related to GT-1

Abstract

Many light-regulated genes contain a conserved GATA motif in their 5'-upstream region. We have characterized in detail the GATA-binding factor, CGF-1, which bonds within a 73 bp TATA-proximal light/circadian regulatory element in the Arabidopsis cab2 promoter and to two more sites farther upstream. CGF-1 was found to be distinct from other metal-dependent GATA-binding factors, but to have the same sequence requirements for binding and similar physical and chemical properties as GT-1, a factor required for light regulation of the tobacco rbcS-3A gene. CGF-1 was found to be constitutively present in extracts and was shown to be immunologically related to GT-1. The close similarity between CGF-1 and GT-1 suggests that a GT-1-like factor is involved in the phytochrome/circadian regulation of the cab2 gene. CGF-1 and GT-1 were also found to have similar sequence specificities to another constitutively-regulated GATA factor, IBF-2b, which binds the I box region of the tomato nitrate reductase gene. Of three complexes detected using an IBF-2b-specific probe, only one was identical to CGF-1/GT-1. The other two were similar to IBF-2b, demonstrating that CGF-1/GT-1, although very similar, are actually distinct from IBF-2b. These data indicate that more than one factor can bind to the same short sequence and may indicate how constitutively present factors like GT-1 can play a role in light regulation.

Differential Regulation of Plastidial and Cytosolic Isoforms of Peptide Methionine Sulfoxide Reductase in Arabidopsis

“…Arabidopsis pmsr has a single intron of 422 nt and two exons of 522 and 252 nt, respectively. There were no obvious TATA boxes near the pmsr transcriptional start site, although a GATA box, which has been shown to play an important role in regulating lightcontrolled genes (Teakle and Kay, 1995), was observed 48 nt upstream of the transcriptional start site. This gene encoded a 258-residue, 28,568-D protein with a putative plastidial targeting signal and was termed p-pmsr.…”

Assessment of miRNA expression profile and differential expression pattern of target genes in cold-tolerant and cold-sensitive tomato cultivars

“…miR172 and miR393 had GATAbox, which has been proposed to regulate light response [64,65] and to be related to cold stress. [8,66] In plants, most ABA-responsive genes have the conserved ABREs in their promoters, which are significant cis-acting elements for genes responsive to abiotic stress in Arabidopsis.…”

Light Regulation of Nitrate uptake, Assimilation and Metabolism

“…Circadian rhythms of the GUS mRNA was retained, and this may indicate that light responsive elements were present within the construct since light responsive and circadian elements may be inseparable . Many light and circadian regulated genes contain GATA motifs in their promoters (Teakle and Kay 1995). Five GATA motifs were found within the 700 bp upstream of the transcription start site, but their role in regulation of transcription of the bean NIA genes has not been clarified (Jensen et al 1996).…”