The GC-MS Analysis of the Essential Oil of Cleome austroarabica

Rehman, Najeeb Ur; Alam, Tanveer; Alhashemi, Shareef Fadhil Mahel; Weli, Afaf M.; Al-Thani, Ghanim Salim; Al-Omar, Wadha Imad; Al‐Harrasi, Ahmed

doi:10.1007/s10600-021-03311-3

Cited by 2 publications

(2 citation statements)

References 13 publications

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“…The vaporizer temperature and ion-source temperature were respectively adjusted to 280 and 230℃. Each chemical composition was identified by comparing the retention index obtained from a database (NIST05) with the retention indexes calculated on the basis of n-alkanes (C9-C46) (Rehman et al, 2021;Chzhu et al, 2020).…”

Section: Gc-ms Analysismentioning

confidence: 99%

Chemical Constituents, Antibacterial and Coagulation Activity of the Essential Oil from the Stem of Artemisia argyle H. Lév.

Shan¹,

Zhou²

2021

American Journal of Biochemistry and Biotechnology

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Artemisia argyle H. Lév. (A. argyle) is a plant of historical importance known as the "mother of herbs" in the Middle Ages. As the leaves of A. argyle have been studied more, but the stems has not been reported. This paper explores the chemical constituents, antibacterial and coagulation activities of the Essential Oil from the Stems of A. argyle (EOSAA) for better exploitation and utilization of resources. EOSAA was extracted by hydrodistillation and its chemical constituents were determined by GC-MS. Escherichia coli, Staphylococcus aureus and Bacillus pumilus were used as test microbial strains to evaluate the antimicrobial potential of EOSAA. Three indicators of Activated Partial Thromboplastin Time (APTT), Prothrombin Time (PT) and Thrombin Time (TT) were used to evaluate the coagulation activity. The extraction rate was 0.20% and 36 compounds were identified, accounting for 93.75% of the total content. Among the identified compounds, 41.72% belong to oxiterpenes, 21.31% belong to sesquiterpenes and terpenoids are extremely abundant. The main constituents were as follows: 3-Biphenylmethanol (16.01%), 2,6-Dimethyl-8-(tetrahydropyran-2yloxy)-octa-2,6-dien-1-ol (9.22%), Germacrene D (7.22%), α-Bisabolol (5.85%), L(-)-Borneol (5.75%), Eucalyptol (5.71%) and so on. EOSAA exhibited certain inhibitory effects against Escherichia coli, Staphylococcus aureus and Bacillus pumilus, the minimum inhibitory concentrations were 40, 42 and 48 mg/mL, respectively. Compared with Yunnan Baiyao, APTT, PT and TT time of high concentration (60 mg/mL) EOSAA were shortened by 28.83, 42.86 and 68.99%, respectively. The experimental results show that EOSAA is a natural coagulant with antibacterial effect and has a broad application prospect.

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Section: Gc-ms Analysismentioning

confidence: 99%

Chemical Constituents, Antibacterial and Coagulation Activity of the Essential Oil from the Stem of Artemisia argyle H. Lév.

Shan¹,

Zhou²

2021

American Journal of Biochemistry and Biotechnology

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“…GC-MS is generally suitable for the analysis of primary metabolites necessary for the survival and reproduction during plant growth and development, such as carbohydrates, proteins, lipids, and nucleic acids, while LC-MS is more suitable for evaluating a large group of plant secondary metabolites including alkaloids, flavonoids, and phenolic compounds (t' Kindt et al, 2009). Most studies use GC-MS or LC-MS technology to determine metabolites based on the characteristics of substances (Muráriková et al, 2017;Rehman et al, 2021); however, it is difficult to obtain all the metabolite information using a single detection technique due to the limitations and biases of the technique (Naz et al, 2014;Gonzalez-Dominguez et al, 2017). A few studies have performed multimetabolome analyses so far (Alves Filhoa et al, 2019;Zhou et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Transcriptome and Metabolome Analyses Reveal Differences in Terpenoid and Flavonoid Biosynthesis in Cryptomeria fortunei Needles Across Different Seasons

Zhang

Yang

et al. 2022

Front. Plant Sci.

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Cryptomeria fortunei (Chinese cedar) has outstanding medicinal value due to its abundant flavonoid and terpenoid contents. The metabolite contents of C. fortunei needles differ across different seasons. However, the biosynthetic mechanism of these differentially synthesized metabolites (DSMs) is poorly understood. To improve our understanding of this process, we performed integrated non-targeted metabolomic liquid chromatography and gas chromatography mass spectrometry (LC-MS and GC-MS), and transcriptomic analyses of summer and winter needles. In winter, the C. fortunei needle ultrastructure was damaged, and the chlorophyll content and Fv/Fm were significantly (p < 0.05) reduced. Based on GC-MS and LC-MS, we obtained 106 and 413 DSMs, respectively; based on transcriptome analysis, we obtained a total of 41.17 Gb of clean data and assembled 33,063 unigenes, including 14,057 differentially expressed unigenes (DEGs). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that these DSMs/DEGs were significantly (p < 0.05) enriched in many biosynthesis pathways, such as terpenoids, photosynthates, and flavonoids. Integrated transcriptomic and metabonomic analyses showed that seasonal changes have the greatest impact on photosynthesis pathways, followed by terpenoid and flavonoid biosynthesis pathways. In summer Chinese cedar (SCC) needles, DXS, DXR, and ispH in the 2-methyl-pentaerythritol 4-phosphate (MEP) pathway and GGPS were highly expressed and promoted the accumulation of terpenoids, especially diterpenoids. In winter Chinese cedar (WCC) needles, 9 genes (HCT, CHS, CHI, F3H, F3'H, F3'5'H, FLS, DFR, and LAR) involved in flavonoid biosynthesis were highly expressed and promoted flavonoid accumulation. This study broadens our understanding of the metabolic and transcriptomic changes in C. fortunei needles caused by seasonal changes and provides a reference regarding the adaptive mechanisms of C. fortunei and the extraction of its metabolites.

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The GC-MS Analysis of the Essential Oil of Cleome austroarabica

Cited by 2 publications

References 13 publications

Chemical Constituents, Antibacterial and Coagulation Activity of the Essential Oil from the Stem of Artemisia argyle H. Lév.

Chemical Constituents, Antibacterial and Coagulation Activity of the Essential Oil from the Stem of Artemisia argyle H. Lév.

Transcriptome and Metabolome Analyses Reveal Differences in Terpenoid and Flavonoid Biosynthesis in Cryptomeria fortunei Needles Across Different Seasons

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