Gender equality is essential for economic and societal advancement, as outlined in the United Nations’ Sustainable Development Goal 5. This research examines the obstacles that hinder women’s progression from higher education to empowerment in Pakistan. Using a comprehensive descriptive survey questionnaire, we collected quantitative data to gain insights from a diverse sample of various provinces. The survey consisted of two sections: background information and 25 constructs of barriers from the existing literature. Data analysis involving 399 respondents included descriptive and inferential statistics, cross-tabulation, and significance testing using non-parametric tests. The findings highlighted significant sociocultural, familial, and professional barriers, with regional disparities underscoring the need for targeted interventions. Women respondents consistently perceived barriers more keenly than men, underscoring the gender-specific nature of these challenges. This study emphasizes the need for focused policies and educational initiatives to address barriers and promote gender equality in Pakistan’s higher education and professional spheres.