The gene encoding ARS-binding factor I is essential for the viability of yeast.

Abstract

The gene encoding a yeast ARS-binding protein, ABF I, has been cloned by screening a genomic Xgtll library using monoclonal and polyclonal antibodies against ABF I. ABF I is of interest because it not only binds to ARSs but also to the 5'-flanking region of genes encoding proteins involved in transcription, translation, respiration, and cell-cycle control. The cloned gene has been used to prepare null mutants, which further demonstrate the importance of the ABF I protein by showing that it is essential for vegetative growth. ABFl maps to chromosome V. The DNA sequence of the ABFl gene reveals several motifs characteristic of DNAbinding proteins but shows no overall similarity to any protein of known function.

TwoSaccharomyces cerevisiaeGenes Which Control Sensitivity To G1Arrest Induced byKluyveromyces lactisToxin:

“…However, the consensus sequence for the P loop of ATP-or GTP-binding proteins (Gly-X4-Gly-Lys-Thr) is present (residues 8 to 15), suggesting that the protein may bind ATP or GTP. We confirmed the previous report that YKL500 is nonessential (42) by inserting the LEU2 coding region into the unique BglII site as described above, excising this YKL500::LEU2 construct from pYF6, and transforming it into LL20 so that it replaced the corresponding genomic locus. Leu+ gene were found to be completely refractory to K lactis toxin.…”

The rRNA enhancer regulates rRNA transcription in Saccharomyces cerevisiae.

“…In addition, there is a thymidine-rich region (5,23,36) which may work either through an inferred binding factor or through its own influence on DNA conformation. Combinations of these three elements are also present within transcriptional control regions of a number of genes transcribed by RNA polymerase II; however, they are usually within 500 bp of the site of transcription initiation (4,5,30,36). The potential involvement of these factors in transcription of both rRNA and mRNA is underscored by the recent finding that the TATA-binding factor is implicated in transcription by all three nuclear RNA polymerases (6,8,33).…”

“…There is a binding site for Reblp, which can also bind upstream of a number of yeast genes transcribed by RNA polymerase II (5,27). There is a weak binding site for Abflp, which acts as a transcription factor (4), a silencing factor (2), and a replication factor (30). There is a 30-bp T-rich stretch, that, either through its influence on DNA structure or through an as-yet-unidentified binding protein, can act as a transcriptional activator, especially in connection with other activators (5,23,31,36).…”

A bipartite DNA-binding domain in yeast Reb1p.

“…Abflp (21,41), Raplp (26,46), and Reblp (7,29,38) all bind to numerous sites within the genome and may play multiple roles in the organization and regulation of transcription of the genome. Whether the extended DNA-binding domain is related to the multiple functions of these proteins remains to be seen.…”