The Gene Expression of Activin Receptors in Rat Pituitary

Aono, Toshihiro; Kuwahara, Akira; Tamura, Noboru; Miyamoto, S; Ushigoe, Kenjiro; Azuma, K; Irahara, Minoru

doi:10.1007/978-1-4612-1874-6_2

Cited by 1 publication

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“…Proteins of this superfamily exert potent growth-regulatory effects on their respective target tissues via a family of specific cell surface receptors. Activin mRNAs are expressed in a variety of tissues, including the ovary, testis, placenta, brain, bone marrow, and pituitary (1), and activin receptor (typeIA, typeII and typeIIB) mRNA expression has been reported in the rat (2,3). Effects of activin on cellular proliferation and/or differentiation have also been observed in primary cultured cells and established cell lines (4)(5)(6).…”

Section: Introductionmentioning

confidence: 99%

Effect of activin on production and secretion of prolactin and growth hormone in cultured rat GH3 cells

Tamura

Irahara²,

Kuwahara³

et al. 2000

European Journal of Endocrinology

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Objective: To evaluate the effect of the growth factor activin A on the secretion of prolactin (PRL) and GH in cultured GH3 cells. Methods:The concentrations of PRL and GH secreted from GH3 cells cultured in media with and without activin A were measured by RIA, and the expression of PRL mRNA and GH mRNA were analyzed using the Northern blot method. Results: Activin A significantly inhibited PRL release from GH3 cells cultured for 48 h in a dosedependent manner (activin: 0.3-3nM). The inhibitory effects of 3nM activin A were observed in the culture from 12 h to 48 h (53.2% of control). Activin A (3nM) also significantly inhibited the expression of PRL mRNA at 24 h (33.8% of control). In contrast, activin A significantly stimulated GH release from GH3 cells cultured for 48 h in a dose-dependent manner (activin: 0.3-3nM). The stimulatory effect of 3nM activin A was observed in the culture for 48 h (157.6% of control). Activin A (3nM) also significantly stimulated the expression of GH mRNA at 24 h (183.6% of control). In spite of these significant changes in PRL and GH secretion, pit-1 mRNA levels were not significantly changed by activin A. Conclusions: These findings indicated that activin A modulates PRL and GH secretion through the regulation of PRL and GH gene transcription in GH3 cells, but that these effects are unrelated to pit-1 gene expression.

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