The Gene Expression Profile by RT-PCR and Immunohistochemical Expression Pattern of Catalase in the Kidney Tissue of Both Healthy and Diabetic Mice

Bingöl, Seyit Ali; Kocamiş, Hakan

doi:10.9775/kvfd.2010.1766

Cited by 9 publications

(9 citation statements)

References 5 publications

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“…Moreover, in our study, reaction being mostly cytoplasmic and less nuclear is consistent with the findings of similar studies [16,29,30] . However, immunoreactivity in the medulla observed by Bingöl and Kocamış [17] could not be observed in our study.…”

Section: Ergin and Başaloğlucontrasting

confidence: 99%

“…Our findings are parallel to the findings of Bingöl and Kocamış [17] , Morikawa et al [29] , Zhou and Kang [30] regarding presence of immunoreactivity in the cortex of the kidney, especially in proximal tubuli in the inner cortex portion and absence of reaction in distal tubuli, vascular endothelium and connective tissue. Kidney and especially proximal KORAL TAŞÇI, DEPREM, BİNGÖL ELİŞ YILDIZ, ASLAN [15] .…”

Section: Ergin and Başaloğlusupporting

confidence: 91%

“…Effect of Melatonin on Catalase ... [28] studied the effect of chronic melatonin injections in kidney tissue and reported that there is no difference histologically, similarly to our findings. In a study by Bingöl and Kocamış [17] on immunohistochemical examination of catalase enzyme in kidney tissues of healthy and diabetic rats, they have reported that catalase immunoreactivity is intense only in proximal tubuli in the cortex of the kidney and quite weak in the medulla, whereas there is no immunoreactivity in structures such as glomeruli, vascular endothelium, distal tubuli. In the same study, they stated that immunoreactivity in the cortex varies by region and immunoreactivity in the inner part of the cortex in the entire groups is more intense.…”

Section: Discussionmentioning

confidence: 99%

“…The task of this enzyme, localized in peroxisomes in the cells, is to transform hydrogen peroxide, which is harmful, into oxygen and water [8,14,15] . This enzyme has been reported to be extensively found  İletişim (Correspondence)  +90 536 3044630  serapkoral@hotmail.com in hepatocytes in the liver [16] and proximal tubules in the kidney [17] . There are important tasks of the kidney tissue on the oxidative metabolism.…”

Section: Introductionmentioning

confidence: 99%

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Melatoninin Fare Böbrek Dokusunda Katalaz Enzimi Üzerine Etkisi

Taşçı¹,

Deprem²,

Bingöl³

et al. 2016

Kafkas Univ Vet Fak Derg

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In this study, we aimed to immunohistochemically investigate the effects of melatonin administration on catalase in the kidney tissue. In our study, 18 male Swiss albino mice were used and they were divided into three groups as control (n=6), sham (n=6) and experimental (n=6). The experimental group received 10 mg/kg dose of melatonin for 28 days (i.p.). Tissue samples were embedded in paraffin. To examine catalase immunoreactivity, avidin-biotin-peroxidase complex (ABC) technique was performed to tissue sections. Based on the results of the immunohistochemical staining, reaction was showed in proximal tubules in all three groups. The reaction in inner cortical proximal tubules was found to be more intense. There was no immunoreactivity in distal tubules, Malpighian bodies, vascular endothelium and structures in the medulla. Catalase immunoreactivity was determined to be more intense in the experimental group than other groups. Our study supports the idea that melatonin could have a direct effect on the kidney tissue and that it could be used as an antioxidant therapeutic agent by strengthening antioxidant mechanism in oxidative stress. Keywords: Catalase, Immunohistochemistry, Kidney, Melatonin, Mouse Melatoninin Fare Böbrek Dokusunda Katalaz Enzimi Üzerine Etkisi ÖzetBu çalışmada, melatonin uygulamasının böbrek dokusunda katalaz üzerine etkisinin immunohistokimyasal olarak araştırılması amaçlandı. Çalışmamızda, 18 adet erkek Swiss albino fare kullanıldı ve bunlar kontrol (n=6), sham (n=6) ve deneme (n=6) olarak üç gruba ayrıldı. Deneme grubuna 28 gün 10 mg/kg dozda melatonin uygulandı (i.p.). Alınan doku örnekleri parafinde bloklandı. Katalaz immunoreaktivitesini incelemek için doku kesitlerine avidin-biotin-peroksidaz kompleks (ABC) tekniği uygulandı. Yapılan incelemeler sonucunda immunohistokimyasal boyamada, üç grupta da reaksiyonun tubulus proksimalislerde olduğu belirlendi. Korteksin iç kısmındaki tubulus proksimalislerde reaksiyonun daha yoğun olduğu görüldü. Tubulus distalislerde, Malpighi cisimciklerinde, damar endotellerinde ve medullada bulunan yapılarda immunoreaktiviteye rastlanmadı. Katalaz immunoreaktivitesinin deneme grubunda, kontrol ve sham grubuna kıyasla daha yoğun olduğu belirlendi. Çalışmamız, melatoninin böbrek dokusu üzerine doğrudan etkisinin olabileceği ve antioksidan mekanizmayı güçlendirerek oksidatif stres durumlarında antioksidan bir terapötik madde olarak kullanılabileceği düşüncelerini desteklemektedir.

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Section: Ergin and Başaloğlucontrasting

confidence: 99%

Section: Ergin and Başaloğlusupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Melatoninin Fare Böbrek Dokusunda Katalaz Enzimi Üzerine Etkisi

Taşçı¹,

Deprem²,

Bingöl³

et al. 2016

Kafkas Univ Vet Fak Derg

Self Cite

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“…The pH of Daucus carrota fortified poultry meat nuggets reduced significantly with successive storage days (Devatkal et al, 2008). However, the findings were in contrast to those resulted on low fat Turkish type meat balls by (Bingol et al, 2010). The FFA showed a significant (p<0.05) th increasing trend on 14 day onwards successive storage days.…”

Section: Resultscontrasting

confidence: 77%

Quality traits of low-sodium fish balls incorporated with barley flour and pea flour

Ganie¹,

Kumar²,

Tanwar³

2017

JEB

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Aim:Methodology: Results:Interpretation:The present study was conducted to analyze the effect of fortification of various variants and combinations of barley flour and pea flour on the quality traits of fish balls.The technical programme was formulated to evaluate and assess the quality traits of low sodium fish balls incorporated with barley and pea flour. The product was developed by replacing ten percent of lean fish flesh (wt/wt) with three different variants and combination of barley flour and pea flour in the following ratios: 20:25, 50:50 and 75:25. The control samples were prepared by incorporating 10% refined wheat flour (wt/wt). The prepared designer emulsion based fish products were packaged hygienically under aerobic condition in 100 gauge polyethylene pouches. All the prepared products were examined for various storage quality traits in refrigerated (4±1 ºC) condition for 21 days at weekly intervals.The physico-chemical quality, emulsion uniformity and stability and uniformity, cooking yield and sensory attributes were found to be significantly (p<0.05) higher with low sodium fish ball product fortified with barley flour and pea flour in 25:75 ratio was optimum and suitable for the preparation of fish balls. The FFA, TBARS and all the microbiological profile were found to be significantly (p<0.05) inclined with the successive storage days.Low sodium fish balls can be optimally and suitably prepared with barley flour and pea flour in 25:75 ratio with substitution of 10% of lean fish meat (wt/wt). The developed products were found to be th safe and fit for human consumption till 14 day of refrigeration storage, without adversely affecting any of its sensory qualities. All the prepared products are kept at refrigeration temperature (4±1°C) under aerobic packaging and analyzed for proximate parameters (Moisture, fats, protein, ash), physio-chemical (pH, cooking yield), sensory attributes, storage quality (FFA, TBA) and microbiological parameters were analyzed at regular intervals at 0, 7,14 and 21 days Low sodium fish ball using barley flour (Hordeum vulgare L.) and pea flour (Pisum sativum) (25:75) was most optimum for preparation without adversely affecting the sensory quality of foods.

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Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

ERDAĞ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:The purpose of this study was to determine the effects of the plant extract, which is obtained from Onosma nigricaule due to the oxidation parameters caused in mice by malathion that is used as an insecticide in agriculture, on catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in kidney tissues by immunohistochemical technique. A total of 48 male mice (Mus musculus) were used in our study. Mice were divided 6 groups (control group, maize oil group, normal saline group, Onosma nigricaule group, malathion group, Onosma nigrcaule+malathion group). Hematoxylin-eosin and triple staining methods were used for histological and pathological examinations. The localization of CAT and Mn-SOD in the renal tissue was determined using the method of streptavidinbiotin-peroxidase. CAT immunoreactivity was determined with a weak intensity in epithelium of renal tubulus proximalis of mice in the malathion group, with a moderate intensity in Onosma nigricaule+malathion group and with a higher intensity in tubulus proximalis of other groups. A cytoplasmic Mn-SOD immunoreactivity was determined with weak intensity in renal medulla of mice in malathion group, with moderate intensity in renal medulla of mice in Onosma nigricaule plant extract+malathion group, maize oil group, and normal saline group and with highly intensity in control and Onosma nigricaule groups. It was concluded that Onosma nigricaule might play a protective role as an antioxidant against the oxidant features of malathion.Keywords: Catalase, kidney, malathion, mice, Onosma nigricaule, superoxide dismutase. Malathion ve Onosma nigricaule (Boraginaceae)'nin fare böbrek dokusunda katalaz (CAT) ve süperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkileriÖzet: Bu çalışmada, tarımda insektisit olarak kullanılan ve farelerde oksidasyon parametrelerine neden olan malathiona karşıOnosma nigricaule'den elde edilen bitki ekstraktlarının immunohistokimyasal yöntemle böbrek dokusunda katalaz (CAT) ve süperoksit dismutaz-2 (Mn-SOD) salınımı üzerine etkilerinin saptanması amaçlanmıştır. Çalışmamızda 48 adet erkek (Mus musculus) fare kullanıldı. Fareler 6 gruba (kontrol grubu, mısır yağı grubu, serum fizyolojik grup, Onosma nigricaule grubu, malathion grubu, Onosma nigricaule+malathion grubu) ayrıldı. Histolojik ve patolojik değerlendirmeler için hematoksilen-eozin and triple boyama yöntemleri kullanıldı. Böbrek dokusunda CAT ve Mn-SOD lokalizasyonu için ise streptavidin-biotin-peroxidase yöntemi kullanıldı. CAT immunoreaktivitesinin fare böbrek dokusunun tubulus proksimalisinde olduğu görüldü. CAT immunoreaktivitesinin malathion grubunda zayıf, malathion+Onosma nigricaule grubunda orta yoğunlukta, diğer gruplarda ise yüksek yoğunlukta olduğu tespit edildi. Mn-SOD immunoreaktivitesinin böbrek medullasında olduğu görüldü. Malathion grubunda zayıf, mısır yağı grubu, serum fizyolojik ve Onosma nigricaule+malathion gruplarında orta yoğunlukta, kontrol ve Onosma nigricaule gruplarında ise yüksek yoğunlukta olduğu tespit edildi. Onosma nigricaule'nin malathionun oksidadif etkile...

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The Gene Expression Profile by RT-PCR and Immunohistochemical Expression Pattern of Catalase in the Kidney Tissue of Both Healthy and Diabetic Mice

Cited by 9 publications

References 5 publications

Melatoninin Fare Böbrek Dokusunda Katalaz Enzimi Üzerine Etkisi

Melatoninin Fare Böbrek Dokusunda Katalaz Enzimi Üzerine Etkisi

Quality traits of low-sodium fish balls incorporated with barley flour and pea flour

Immunohistochemical examination on the effects of malathion and Onosma nigricaule (Boraginaceae) on the catalase (CAT) and superoxide dismutase-2 (Mn-SOD) in renal tissues of mice

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