The Gene
            <i>yggE</i>
            Functions in Restoring Physiological Defects of
            <i>Escherichia coli</i>
            Cultivated under Oxidative Stress Conditions

Kim, Sun Young; Nishioka, Motomu; Hayashi, Shogo; Honda, Hiroyuki; Kobayashi, Takeshi; Taya, Masahito

doi:10.1128/aem.71.5.2762-2765.2005

Cited by 26 publications

(22 citation statements)

References 22 publications

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“…The results confirmed that exposure to nTiO 2 affects algal growth [3] and photosynthetic activity [12] and that abiotic parameters, such as particle size/aggregation and illumination, are key factors affecting nTiO 2 toxicity [15,16]. The underlying toxicity mechanisms of nTiO 2 nanomaterials have been elucidated to some extent, and they include membrane disruption [17], protein oxidation via reactive oxygen species (ROS) formation [9], and possible DNA damage [18]. Furthermore, persistence and bioaccumulation of nTiO 2 in cells is mostly unknown, and this potentially presents a concern for possible introduction into the food web.…”

Section: Introductionsupporting

confidence: 72%

Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization

Cherchi

Chernenko

Diem

et al. 2011

Environmental Toxicology and Chemistry

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The present study investigated the impact of nano titanium dioxide (nTiO(2) ) exposure on the cellular structures of the nitrogen-fixing cyanobacteria Anabaena variabilis. Results of the present study showed that nTiO(2) exposure led to observable alteration in various intracellular structures and induced a series of recognized stress responses, including production of reactive oxygen species (ROS), appearance and increase in the abundance of membrane crystalline inclusions, membrane mucilage layer formation, opening of intrathylakoidal spaces, and internal plasma membrane disruption. The production of total ROS in A. variabilis cells increased with increasing nTiO(2) doses and exposure time, and the intracellular ROS contributed to only a small fraction (<10%) of the total ROS measured. The percentage of cells with loss of thylakoids and growth of membrane crystalline inclusions increased as the nTiO(2) dose and exposure time increased compared with controls, suggesting their possible roles in stress response to nTiO(2) , as previously shown for metals. Algal cell surface morphology and mechanical properties were modified by nTiO(2) exposure, as indicated by the increase in cell surface roughness and shifts in cell spring constant determined by atomic force microscopy analysis. The change in cell surface structure and increase in the cellular turgor pressure likely resulted from the structural membrane damage mediated by the ROS production. Transmission electron microscopy (TEM) analysis of nTiO(2) aggregates size distribution seems to suggest possible disaggregation of nTiO(2) aggregates when in close contact with microbial cells, potentially as a result of biomolecules such as DNA excreted by organisms that may serve as a biodispersant. The present study also showed, for the first time, with both TEM and Raman imaging that internalization of nTiO(2) particles through multilayered membranes in algal cells is possible. Environ. Toxicol. Chem. 2011; 30:861-869. © 2010 SETAC.

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Section: Introductionsupporting

confidence: 72%

Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization

Cherchi

Chernenko

Diem

et al. 2011

Environmental Toxicology and Chemistry

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“…Moreover, spontaneously derived superoxide dismutase (SOD)-deficient E. coli cells induce yggE (23). Therefore, yggE is proposed to act as an auxiliary defense system against oxidative stress (23). In accordance with this notion, intracellular ROS levels were lower in SOD-deficient E. coli strains that overexpressed yggE than in E. coli strains that did not do so (25).…”

Section: Discussionsupporting

confidence: 67%

“…YggE has been predicted to function as an auxiliary defense system against oxidative stress (23). We therefore hypothesized that YggE is a fitness factor for E. coli Nissle.…”

Section: Resultsmentioning

confidence: 99%

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Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model

Schumann

Alpert

Engst

et al. 2012

Appl Environ Microbiol

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To identify Escherichia coli proteins involved in adaptation to intestinal inflammation, mice were monoassociated with the colitogenic E. coli strain UNC or with the probiotic E. coli strain Nissle. Intestinal inflammation was induced by treating the mice with 3.5% dextran sodium sulfate (DSS). Differentially expressed proteins in E. coli strains collected from cecal contents were identified by 2-dimensional difference gel electrophoresis. In both strains, acute inflammation led to the downregulation of pathways involved in carbohydrate breakdown and energy generation. Accordingly, DSS-treated mice had lower concentrations of bacterial fermentation products in their cecal contents than control mice. Differentially expressed proteins also included the Fe-S cluster repair protein NfuA, the tryptophanase TnaA, and the uncharacterized protein YggE. NfuA expression was 3-fold higher in E. coli strains from DSS-treated than from control mice. Reporter experiments confirmed the induction of nfuA in response to iron deprivation, mimicking Fe-S cluster destruction by inflammation. YggE expression, which has been reported to reduce the intracellular level of reactive oxygen species, was 4-to 8-fold higher in E. coli Nissle than in E. coli UNC. This was confirmed by in vitro reporter gene assays indicating that Nissle is better equipped to cope with oxidative stress than UNC. Nissle isolated from DSS-treated and control mice had TnaA levels 4-to 7-fold-higher than those of UNC. Levels of indole resulting from the TnaA reaction were higher in control animals associated with E. coli Nissle. Because of its anti-inflammatory effect, indole is hypothesized to be involved in the extension of the remission phase in ulcerative colitis described for E. coli Nissle.

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“…The cytoplasmic YajQ protein was the single additional protein modified only during UV-A photocatalysis and not with TiO 2 in the dark, in comparison to the others, which were already modified in the dark (TiO 2 cytotoxic effect). The YggE gene product is involved in the reduction of the intracellular ROS level and is therefore responsible for lowering the concentration of ROS to a tolerated level (47). ArcA is a two-component response regulator involved in the oxidative stress response (48,49), and YajQ is involved in ROS detoxification mechanism (50).…”

Section: Discussionmentioning

confidence: 99%

TiO ₂ Photocatalysis Damages Lipids and Proteins in Escherichia coli

Carré

Hamon

Ennahar

et al. 2014

Appl Environ Microbiol

200

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e This study investigates the mechanisms of UV-A (315 to 400 nm) photocatalysis with titanium dioxide (TiO 2 ) applied to the degradation of Escherichia coli and their effects on two key cellular components: lipids and proteins. The impact of TiO 2 photocatalysis on E. coli survival was monitored by counting on agar plate and by assessing lipid peroxidation and performing proteomic analysis. We observed through malondialdehyde quantification that lipid peroxidation occurred during the photocatalytic process, and the addition of superoxide dismutase, which acts as a scavenger of the superoxide anion radical (O 2 · ؊ ), inhibited this effect by half, showing us that O 2 · ؊ radicals participate in the photocatalytic antimicrobial effect. Qualitative analysis using twodimensional electrophoresis allowed selection of proteins for which spot modifications were observed during the applied treatments. Two-dimensional electrophoresis highlighted that among the selected protein spots, 7 and 19 spots had already disappeared in the dark in the presence of 0.1 g/liter and 0.4 g/liter TiO 2 , respectively, which is accounted for by the cytotoxic effect of TiO 2 . Exposure to 30 min of UV-A radiation in the presence of 0.1 g/liter and 0.4 g/liter TiO 2 increased the numbers of missing spots to 14 and 22, respectively. The proteins affected by photocatalytic oxidation were strongly heterogeneous in terms of location and functional category. We identified several porins, proteins implicated in stress response, in transport, and in bacterial metabolism. This study reveals the simultaneous effects of O 2 · ؊ on lipid peroxidation and on the proteome during photocatalytic treatment and therefore contributes to a better understanding of molecular mechanisms in antibacterial photocatalytic treatment.

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The Gene yggE Functions in Restoring Physiological Defects of Escherichia coli Cultivated under Oxidative Stress Conditions

Cited by 26 publications

References 22 publications

Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization

Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization

Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model

TiO ₂ Photocatalysis Damages Lipids and Proteins in Escherichia coli

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The Gene yggE Functions in Restoring Physiological Defects of Escherichia coli Cultivated under Oxidative Stress Conditions

Cited by 26 publications

References 22 publications

Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization

Impact of nano titanium dioxide exposure on cellular structure of Anabaena variabilis and evidence of internalization

Dextran Sodium Sulfate-Induced Inflammation Alters the Expression of Proteins by Intestinal Escherichia coli Strains in a Gnotobiotic Mouse Model

TiO 2 Photocatalysis Damages Lipids and Proteins in Escherichia coli

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TiO ₂ Photocatalysis Damages Lipids and Proteins in Escherichia coli