The gene profile of <i>Enterobacteriaceae</i> virulence factors in relation to bacteriuria levels between the acute episodes of recurrent uncomplicated lower urinary tract infection

Naboka, Y.L.; Mavzyutov, A. R.; Kogan, M. I.; Гудима, И А; Dzhalagoniya, K.T.; Иванов, С. Н.; Naber, Kurt G.

doi:10.1080/14787210.2021.1866986

Cited by 8 publications

(7 citation statements)

References 30 publications

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“…In contrast, the dominant microbial taxa of OA1 cluster was Enterobacteriaceae (over 80% on average), which contained several pathogens such as E. coli , Klebsiella pneumoniae , and Salmonella enterica . These bacteria are often reported to be related to UTI, intestinal inflammation, and multi-drug resistance and are the most common nosocomial pathogens ( Naboka et al., 2021 ). In terms of clinical indexes, the urine WBC/HP level of the OA1 cluster was much higher than the OA2 cluster, which may be due to the differential relative abundance of Enterobacteriaceae in OA1 and OA2 clusters.…”

Section: Discussionmentioning

confidence: 99%

Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

Shen

Zhu

Fan

et al. 2021

Front. Cell. Infect. Microbiol.

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Urinary stones and urinary tract infection (UTI) are the most common diseases in urology and they are characterized by high incidence and high recurrence rate in China. Previous studies have shown that urinary stones are closely associated with gut or urine microbiota. Calcium oxalate stones are the most common type of urinary stones. However, the profile of urinary tract microorganisms of calcium oxalate stones with UTI is not clear. In this research, we firstly found two novel clusters in patients with calcium oxalate stones (OA) that were associated with the WBC/HP (white blood cells per high-power field) level in urine. Two clusters in the OA group (OA1 and OA2) were distinguished by the key microbiota Firmicutes and Enterobacteriaceae. We found that Enterobacteriaceae enriched in OA1 cluster was positively correlated with several infection-related pathways and negatively correlated with a few antibiotics-related pathways. Meantime, some probiotics with higher abundance in OA2 cluster such as Bifidobacterium were positively correlated with antibiotics-related pathways, and some common pathogens with higher abundance in OA2 cluster such as Enterococcus were positively correlated with infection-related pathways. Therefore, we speculated that as a sub-type of OA disease, OA1 was caused by Enterobacteriaceae and the lack of probiotics compared with OA2 cluster. Moreover, we also sequenced urine samples of healthy individuals (CK), patients with UTI (I), patients with uric acid stones (UA), and patients with infection stones (IS). We identified the differentially abundant taxa among all groups. We hope the findings will be helpful for clinical treatment and diagnosis of urinary stones.

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Section: Discussionmentioning

confidence: 99%

Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

Shen

Zhu

Fan

et al. 2021

Front. Cell. Infect. Microbiol.

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“…Методика подготовки проб исходных образцов для ПЦР, выделения ДНК и список использованных праймеров, синтезированных в ООО «Биоскрин» (Уфа), «Синтол» (Москва), представлены в работе Naboka et al [7]. Данные праймеры были специфичны генам papA (субъединица P пилей), papE/F (поверхностный адгезин P пилей), papGII (поверхностный адгезин P пилей аллель II), afa (афимбрийный адгезин), bmaE (М пили), fyuA, iutA, feoB (гены белков системы сидерофоров), kpsMII (капсулообразование) и usp (уропатогенный специфический белок).…”

Section: бактериальная инфекция нижних мочевых путейunclassified

“…Рецидивы НИНМП могут быть обусловлены как реинфекцией, так и персистенцией бактерий, вызвавших первичное инфицирование. Дискуссия по этому вопросу продолжается десятилетиями [7,[11][12][13]. Исследования Ejrnaes et al [14] показали, что в 77.0% случаев очередной рецидив НИНМП связан с ранее выявленным штаммом, а в 23.3% -новым штаммом E. coli в группе пациентов, получавших терапию пивмециллином.…”

Section: обсуж дениеunclassified

[RETRACTED: Биологические Свойства Грамнегативных Бактерий, Верифицированных В Моче Пациенток С Рецидивирующей Неосложненной Инфекцией Нижних Мочевых Путей]

Naboka

Mavzyutov

Коган

et al. 2022

Microbiology Independent Research Journal (MIR Journal)

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Неосложненные инфекции нижних мочевых путей чаще регистрируются у женщин и характеризуются рецидивирующим тяжелым течением. Отсутствие эффективных подходов по ведению данной когорты пациенток и недостаточная изученность качественных характеристик уропатогенов препятствуют успешному лечению этих инфекций. Цель настоящего исследования -изучить биологические свойства представителей грамнегативной микробиоты, выделенной из мочи пациенток с рецидивирующей неосложненной инфекцией нижних мочевых путей, при различных уровнях бактериурии. Проведено когортное одномоментное исследование 62 пациенток в возрасте 20-50 лет с рецидивирующей неосложненной инфекцией нижних мочевых путей. У грамнегативных бактерий, выделенных из мочи пациенток, определяли уровень бактериурии, гены факторов вирулентности, гемолитическую, адгезивную и антилизоцимную активности. У всех пациенток из мочи выделены представители грамнегативных микроорганизмов (62 штамма). Уровни бактериурии колебались от 10 2 до 10 8 КОЕ/мл. У всех штаммов обнаружены гены факторов вирулентности в широком диапазоне с превалированием papGII, feoB, fyuA независимо от степени бактериурии. Обнаружено 20 достоверных (p<0.05) коэффициентов взаимной сопряженности между различными парами генов. Гемолитическая активность энтеробактерий значимо связана с papA, feoB, fyuA, kpsMII и usp, антилизоцимная -с papA, afa и usp. Таким образом, наличие генотипических и некоторых фенотипических признаков в различных вариациях экспрессии у всех представителей грамнегативных бактерий при всех уровнях бактериурии создает потенциальный риск развития очередного рецидива инфекции нижних мочевых путей. Поэтому даже минимальные уровни бактериурии являются клинически значимыми. Таким образом, при бактериологической диагностике необходимо определять все уровни бактериурии, включая минимальный (10 2 КОЕ/мл), так как выделенные микроорганизмы при данном уровне обладают не только адгезивной и антилизоцимной активностями, но и имеют гены факторов вирулентности.

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“…The pathogenicity of Enterobacteriaceae in urinary tract infections increases with the presence of virulence factors. Indeed, Enterobacteriaceae strains harbor several virulence genes associated with serious or recurrent urinary tract infections [ 15 ]. Among these genes, P fimbriae ( pap ), S-fimbriae ( sfa ), hemolysins ( hly ), cytotoxic-necrotizing-factor ( cnf1 ) and Aerobactin ( iucD ) are the most relevant [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility and Virulence of Enterobacteriaceae Isolated from Urinary Tract Infections in Benin

et al. 2023

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Enterobacteriaceae represent one of the main families of Gram-negative bacilli responsible for serious urinary tract infections (UTIs). The present study aimed to define the resistance profile and the virulence of Enterobacteriaceae strains isolated in urinary tract infections in Benin. A total of 390 urine samples were collected from patients with UTIs, and Enterobacteriaceae strains were isolated according to standard microbiology methods. The API 20E gallery was used for biochemical identification. All the isolated strains were subjected to antimicrobial susceptibility testing using the disc diffusion method. Extended-spectrum beta-lactamase (ESBL) production was investigated using a double-disc synergy test (DDST), and biofilm production was quantified using the microplate method. Multiplex PCR was used to detect uro-virulence genes, namely: PapG, IronB, Sfa, iucD, Hly, FocG, Sat, FyuA and Cnf, using commercially designed primers. More than 26% (103/390) of our samples were contaminated by Enterobacteriaceae strains at different levels. Thus, E. coli (31.07%, 32/103), Serratia marcescens (11.65%, 12/103), Klebsiella ornithinolytica (8.74%, 9/103), Serratia fonticola (7.77%, 8/103) and Enterobacter cloacae (6.80%, 7/103) were identified. Among the isolated strains, 39.81% (41/103) were biofilm-forming, while 5.83% (6/103) were ESBL-producing. Isolates were most resistant to erythromycin, cefixime, ceftriaxone and ampicillin (≥90%) followed by ciprofloxacin, gentamycin, doxycycline and levofloxacin (≥50%), and least resistant to imipenem (27.18%). In regard to virulence genes, Sfa was the most detected (28.15%), followed by IronB (22.23%), iucD (21.36%), Cnf (15.53%), PapG (9.71%), FocG (8.74%), Sat (6.79%), FyuA (5.82%) and Hyl (2.91%). These data may help improve the diagnosis of uropathogenic strains of Enterobacteriaceae, but also in designing effective strategies and measures for the prevention and management of severe, recurrent, or complicated urinary tract infections in Benin.

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The gene profile of Enterobacteriaceae virulence factors in relation to bacteriuria levels between the acute episodes of recurrent uncomplicated lower urinary tract infection

Cited by 8 publications

References 30 publications

Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

Identifying Two Novel Clusters in Calcium Oxalate Stones With Urinary Tract Infection Using 16S rDNA Sequencing

[RETRACTED: Биологические Свойства Грамнегативных Бактерий, Верифицированных В Моче Пациенток С Рецидивирующей Неосложненной Инфекцией Нижних Мочевых Путей]

Susceptibility and Virulence of Enterobacteriaceae Isolated from Urinary Tract Infections in Benin

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