The Generation of Influenza-Specific Humoral Responses Is Impaired in ST6Gal I-Deficient Mice

Zeng, Junwei; Joo, Hye Mee; Rajini, Bheemreddy; Wrammert, Jens; Sangster, Mark Y.; Onami, Thandi M.

doi:10.4049/jimmunol.0802833

Cited by 15 publications

(13 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…ϩ but not CD4 ϩ T cells in the spleen after influenza virus infection (58). Thus, it is possible that a lack of binding to CD22 by T cells and NK cells in Cd22 Ϫ/Ϫ mice leads to defective entry into or reduced retention of these cells within sites of WNV infection.…”

Section: Discussionmentioning

confidence: 99%

CD22 Is Required for Protection against West Nile Virus Infection

Daphne

Suthar

Kasahara

et al. 2013

J Virol

View full text Add to dashboard Cite

West Nile virus (WNV) is a RNA virus of the family Flaviviridae and the leading cause of mosquito-borne encephalitis in the United States. Humoral immunity is essential for protection against WNV infection; however, the requirements for initiating effective antibody responses against WNV infection are still unclear. CD22 (Siglec-2) is expressed on B cells and regulates B cell receptor signaling, cell survival, proliferation, and antibody production. In this study, we investigated how CD22 contributes to protection against WNV infection and found that CD22 knockout (Cd22 ؊/؊ ) mice were highly susceptible to WNV infection and had increased viral loads in the serum and central nervous system (CNS) compared to wildtype (WT) mice. This was not due to a defect in humoral immunity, as Cd22 ؊/؊ mice had normal WNV-specific antibody responses. However, Cd22 ؊/؊ mice had decreased WNV-specific CD8 ؉ T cell responses compared to those of WT mice. These defects were not simply due to reduced cytotoxic activity or increased cell death but, rather, were associated with decreased lymphocyte migration into the draining lymph nodes (dLNs) of infected Cd22 ؊/؊ mice. Cd22 ؊/؊ mice had reduced production of the chemokine CCL3 in the dLNs after infection, suggesting that CD22 affects chemotaxis via controlling chemokine production. CD22 was not restricted to B cells but was also expressed on a subset of splenic DCIR2؉ dendritic cells that rapidly expand early after WNV infection. Thus, CD22 plays an essential role in controlling WNV infection by governing cell migration and CD8 ؉ T cell responses.

show abstract

Section: Discussionmentioning

confidence: 99%

CD22 Is Required for Protection against West Nile Virus Infection

Daphne

Suthar

Kasahara

et al. 2013

J Virol

View full text Add to dashboard Cite

show abstract

“…CD22 engagement in trans with CD22 ligands on T cells may regulate T cell activation [12,13]. Mice unable to express CD22 ligands (ST6GalI -/- mice) have normal T cells but defective TD Ab responses to Ag + adjuvant or influenza infection [14,15]. Finally, in addition to inhibition of BCR signaling through surface IgM and IgD [6–8], CD22 also affects intracellular free calcium released by B cells expressing IgG [16,17].…”

Section: Introductionmentioning

confidence: 99%

CD22 is required for formation of memory B cell precursors within germinal centers

2017

View full text Add to dashboard Cite

CD22 is a BCR co-receptor that regulates B cell signaling, proliferation and survival and is required for T cell-independent Ab responses. To investigate the role of CD22 during T cell-dependent (TD) Ab responses and memory B cell formation, we analyzed Ag-specific B cell responses generated by wild-type (WT) or CD22-/- B cells following immunization with a TD Ag. CD22-/- B cells mounted normal early Ab responses yet failed to generate either memory B cells or long-lived plasma cells, whereas WT B cells formed both populations. Surprisingly, B cell expansion and germinal center (GC) differentiation were comparable between WT and CD22-/- B cells. CD22-/- B cells, however, were significantly less capable of generating a population of CXCR4hiCD38hi GC B cells, which we propose represent memory B cell precursors within GCs. These results demonstrate a novel role for CD22 during TD humoral responses evident during primary GC formation and underscore that CD22 functions not only during B cell maturation but also during responses to both TD and T cell-independent antigens.

show abstract

“…The Saa genes produce acute-phase proteins in response to inflammatory stimuli (16) and are associated with insulin resistance in mice (47). Several other genes in this cluster have also been implicated in inflammation or the immune system, including lipocalin (Lcn)2 (63), intercellular adhesion molecule (Icam)1 (26), orosomucoid (Orm)1 (48), orosomucoid (Orm)2 (6), LPS-induced TNF-␣ factor (Litaf) (53), and ␤-galactoside ␣2,6 sialyltransferase (St6gal)1 (62). Even though other genes in this cluster have been not implicated in the inflammatory response, their strong correlation may suggest that they may also be involved in immune response in liver.…”

Section: Correlation Analysis Reveals Sex-specific Gene Expression Nementioning

confidence: 99%

Sex-specific gene expression in the BXD mouse liver

Gatti

Zhao

Chesler

et al. 2010

Physiological Genomics

View full text Add to dashboard Cite

Differences in clinical phenotypes between the sexes are well documented and have their roots in differential gene expression. While sex has a major effect on gene expression, transcription is also influenced by complex interactions between individual genetic variation and environmental stimuli. In this study, we sought to understand how genetic variation affects sex-related differences in liver gene expression by performing genetic mapping of genomewide liver mRNA expression data in a genetically defined population of naive male and female mice from C57BL/6J, DBA/2J, B6D2F1, and 37 C57BL/6J × DBA/2J (BXD) recombinant inbred strains. As expected, we found that many genes important to xenobiotic metabolism and other important pathways exhibit sexually dimorphic expression. We also performed gene expression quantitative trait locus mapping in this panel and report that the most significant loci that appear to regulate a larger number of genes than expected by chance are largely sex independent. Importantly, we found that the degree of correlation within gene expression networks differs substantially between the sexes. Finally, we compare our results to a recently released human liver gene expression data set and report on important similarities in sexually dimorphic liver gene expression between mouse and human. This study enhances our understanding of sex differences at the genome level and between species, as well as increasing our knowledge of the molecular underpinnings of sex differences in responses to xenobiotics.

show abstract

The Generation of Influenza-Specific Humoral Responses Is Impaired in ST6Gal I-Deficient Mice

Cited by 15 publications

References 40 publications

CD22 Is Required for Protection against West Nile Virus Infection

CD22 Is Required for Protection against West Nile Virus Infection

CD22 is required for formation of memory B cell precursors within germinal centers

Sex-specific gene expression in the BXD mouse liver

Contact Info

Product

Resources

About