During 2003 the IEN-PTB Two-Way Satellite Time and Frequency Transfer (TWSTFT) link was calibrated using a portable reference station. The calibration activity was conducted in the framework of the Galileo System Test Bed Version 1 (GSTB V1), under a contract with Joanneum Research G.m.b.H. (Austria). The calibration constant was determined with an uncertainty lower than 1 ns. Only few months after the calibration, the satellite provider moved the TWSTFT service to a different satellite; this caused changes non-reciprocal delays of the link (Sagnac and earth stations delays) and the calibration constant had to be re-evaluated. A recalculation of the Sagnac delays is presented together with a re-evaluation of earth stations delays with different measurements techniques.