1983
DOI: 10.1093/nar/11.16.5347
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The genes coding for histone H3 and H4 inNeurospora crassaare unique and contain intervening sequences

Abstract: Sequences coding for histone H3 and H4 of Neurospora crassa could be identified in genomic digests with the use of the corresponding genes from sea urchin and X. laevis as hybridization probes. A 2.6 kb HindIII-generated N. crassa DNA fragment, showing homology with the heterologous histone H3-gene probes was cloned in a charon 21A vector. Using DNA from this clone as a homologous hybridization probe a 6.9 kb SalI-generated DNA fragment was isolated which in addition to the histone H3-gene also contains the ge… Show more

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Cited by 138 publications
(60 citation statements)
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“…An additional consensus sequence, PuCTPuAC, was found within and near the 3' terminus of all intervening sequences of the glucoamylase gene. An identical sequence is similarly located within intervening sequences of other filamentous ascomycetes (T. reesei [54] and N. crassa [26,67]) and is related to the consensus sequence TACTAAC found within intervening sequences of S. cerevisiae genes (30). This latter sequence has been postulated to be required for RNA splicing in S.…”
Section: Resultsmentioning
confidence: 90%
See 1 more Smart Citation
“…An additional consensus sequence, PuCTPuAC, was found within and near the 3' terminus of all intervening sequences of the glucoamylase gene. An identical sequence is similarly located within intervening sequences of other filamentous ascomycetes (T. reesei [54] and N. crassa [26,67]) and is related to the consensus sequence TACTAAC found within intervening sequences of S. cerevisiae genes (30). This latter sequence has been postulated to be required for RNA splicing in S.…”
Section: Resultsmentioning
confidence: 90%
“…The intervening sequences were short (ranging from 55 to 75 bp) and were all located within protein-encoding sequences. Short intervening sequences have also been found in genes of other filamentous ascomycetes, namely, the cellobiohydrolase gene of Trichoderma reesei (54) and the glutamate dehydrogenase gene (26) and histone H3 and H4 genes (67) of Neurospora crassa. In contrast, the trp-J gene of N. crassa has been shown to lack intervening sequences (50 5' end of the glucoamylase gene was hybridized to total poly(A)+ mRNA from starchgrown cells, and S1 nuclease-resistant products were analyzed by gel electrophoresis under denatured conditions (49).…”
Section: Resultsmentioning
confidence: 99%
“…That presumed d(ATG) start codon is preceeded by a short sequence d(CACA) similar to the start codon of other Neurospora crassa mRNAs [13,15,[22][23][24][25]. The start of the mRNA has not yet been determined.…”
Section: Isolation Of Cloned Cdma and Genomic Dnamentioning
confidence: 99%
“…The incomplete unit structure and dispersed organization of histone genes in genomes of plants imply that highly diverged flanking regions can exist near histone genes. In the H3 gene, although introns are found only in the H3.3 variant of plants (Chaboute et al, 1992), animals (Akhmanova et al, 1995;, and some lower eukaryotes (Muller and Schmitt, 1988;Woudt et al, 1983;Ehinger et al,1990), the H3 genes of liverwort contain introns of various types (Fig. 3).…”
Section: Discussionmentioning
confidence: 99%