The genes encoding the phospho- and matrix proteins of phocine distemper virus

Curran, Martin D.; Rima, Bertus K.

doi:10.1099/0022-1317-73-6-1587

Cited by 14 publications

(3 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another protein, V, is also encoded by the P gene sequence in these viruses. The V protein is translated from an edited mRNA template (Cattaneo et al 1989;Galinski and Wechsler 1991;Curran and Rima 1992;Yamanaka et al 1992). Translation of V begins at the same FIG.…”

Section: Introductionmentioning

confidence: 99%

Molecular Evolution of the Paramyxoviridae and Rhabdoviridae Multiple-Protein-Encoding P Gene

Jordan¹,

Sutter²,

McClure³

2000

Molecular Biology and Evolution

View full text Add to dashboard Cite

Presented here is an analysis of the molecular evolutionary dynamics of the P gene among 76 representative sequences of the Paramyxoviridae and Rhabdoviridae RNA virus families. In a number of Paramyxoviridae taxa, as well as in vesicular stomatitis viruses of the Rhabdoviridae, the P gene encodes multiple proteins from a single genomic RNA sequence. These products include the phosphoprotein (P), as well as the C and V proteins. The complexity of the P gene makes it an intriguing locus to study from an evolutionary perspective. Amino acid sequence alignments of the proteins encoded at the P and N loci were used in independent phylogenetic reconstructions of the Paramyxoviridae and Rhabdoviridae families. P-gene-coding capacities were mapped onto the Paramyxoviridae phylogeny, and the most parsimonious path of multiple-coding-capacity evolution was determined. Levels of amino acid variation for Paramyxoviridae and Rhabdoviridae P-gene-encoded products were also analyzed. Proteins encoded in overlapping reading frames from the same nucleotides have different levels of amino acid variation. The nucleotide architecture that underlies the amino acid variation was determined in order to evaluate the role of selection in the evolution of the P gene overlapping reading frames. In every case, the evolution of one of the proteins encoded in the overlapping reading frames has been constrained by negative selection while the other has evolved more rapidly. The integrity of the overlapping reading frame that represents a derived state is generally maintained at the expense of the ancestral reading frame encoded by the same nucleotides. The evolution of such multicoding sequences is likely a response by RNA viruses to selective pressure to maximize genomic information content while maintaining small genome size. The ability to evolve such a complex genomic strategy is intimately related to the dynamics of the viral quasispecies, which allow enhanced exploration of the adaptive landscape.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular Evolution of the Paramyxoviridae and Rhabdoviridae Multiple-Protein-Encoding P Gene

Jordan¹,

Sutter²,

McClure³

2000

Molecular Biology and Evolution

View full text Add to dashboard Cite

show abstract

“…Considering such astonishing coding strategies of these viruses, it seems unlikely that a virus would retain wasteful genome regions throughout its evolutional history. Nevertheless, morbillivirus genomes, unlike the genomes of other paramyxoviruses, have unusually long M 3Ј UTRs and F 5Ј UTRs (1,2,6,7,9,14,15,19,23,32,33,39,45). In this study, we showed that these long UTRs per se were not essential for MeV replication, but that alteration or deletion of these long UTRs influenced virus replication and cytopathogenicity.…”

Section: Discussionmentioning

confidence: 61%

“…In paramyoviruses, mRNAs generally contain open reading frames (ORFs) with short 5Ј and 3Ј untranslated regions (UTRs). However, the M and F mRNAs of morbilliviruses have unusually long 3Ј and 5Ј UTRs, respectively (1,2,6,7,9,14,15,19,23,32,33,39,45). One exception is the canine distemper virus (CDV) F gene.…”

mentioning

confidence: 99%

Long Untranslated Regions of the Measles Virus M and F Genes Control Virus Replication and Cytopathogenicity

Takeda

Ohno

Seki

et al. 2005

J Virol

115

View full text Add to dashboard Cite

Measles is still a major cause of mortality mainly in developing countries. The causative agent, measles virus (MeV), is an enveloped virus having a nonsegmented negative-sense RNA genome, and belongs to the genus Morbillivirus of the family Paramyxoviridae. One feature of the moribillivirus genomes is that the M and F genes have long untranslated regions (UTRs). The M and F mRNAs of MeV have 426-nucleotide-long 3' and 583-nucleotide-long 5' UTRs, respectively. Though these long UTRs occupy as much as approximately 6.4% of the virus genome, their function remains unknown. To elucidate the role of the long UTRs in the context of virus infection, we used the reverse genetics based on the virulent strain of MeV, and generated a series of recombinant viruses having alterations or deletions in the long UTRs. Our results showed that these long UTRs per se were not essential for MeV replication, but that they regulated MeV replication and cytopathogenicity by modulating the productions of the M and F proteins. The long 3' UTR of the M mRNA was shown to have the ability to increase the M protein production, promoting virus replication. On the other hand, the long 5' UTR of the F mRNA was found to possess the capacity to decrease the F protein production, inhibiting virus replication and yet greatly reducing cytopathogenicity. We speculate that the reduction in cytopathogenicity may be advantageous for MeV fitness and survival in nature.

show abstract

Involvement of morbilliviruses in the pathogenesis of demyelinating disease

Sips

Chesik

Glazenburg

et al. 2007

Reviews in Medical Virology

View full text Add to dashboard Cite

Two members of the morbillivirus genus of the family Paramyxoviridae, canine distemper virus (CDV) and measles virus (MV), are well-known for their ability to cause a chronic demyelinating disease of the CNS in their natural hosts, dogs and humans, respectively. Both viruses have been studied for their potential involvement in the neuropathogenesis of the human demyelinating disease multiple sclerosis (MS). Recently, three new members of the morbillivirus genus, phocine distemper virus (PDV), porpoise morbillivirus (PMV) and dolphin morbillivirus (DMV), have been discovered. These viruses have also been shown to induce multifocal demyelinating disease in infected animals. This review focuses on morbillivirus-induced neuropathologies with emphasis on aetiopathogenesis of CNS demyelination. The possible involvement of a morbillivirus in the pathogenesis of multiple sclerosis is discussed.

show abstract

The genes encoding the phospho- and matrix proteins of phocine distemper virus

Cited by 14 publications

References 21 publications

Molecular Evolution of the Paramyxoviridae and Rhabdoviridae Multiple-Protein-Encoding P Gene

Molecular Evolution of the Paramyxoviridae and Rhabdoviridae Multiple-Protein-Encoding P Gene

Long Untranslated Regions of the Measles Virus M and F Genes Control Virus Replication and Cytopathogenicity

Involvement of morbilliviruses in the pathogenesis of demyelinating disease

Contact Info

Product

Resources

About