The genetic basis for organophosphorus resistance in the Australian sheep blowfly, <i>Lucilia cuprina</i> (Wiedemann) (Diptera, Calliphoridae)

Arnold, James T.; Whitten, M. J.

doi:10.1017/s000748530001066x

Cited by 40 publications

(24 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Synergistic (greater than additive) gene interactions have been noted previously in pesticide-resistant strains of insects (Arnold and Whitten, 1976;Raymond et al, 1989;Shono et al, 2002). However, examples of field-collected strains containing synergistically interacting genes are very rare.…”

Section: Discussionmentioning

confidence: 91%

Gene interactions constrain the course of evolution of phosphine resistance in the lesser grain borer, Rhyzopertha dominica

et al. 2008

View full text Add to dashboard Cite

Phosphine, a widely used fumigant for the protection of stored grain from insect pests, kills organisms indirectly by inducing oxidative stress. High levels of heritable resistance to phosphine in the insect pest of stored grain, Rhyzopertha dominica have been detected in Asia, Australia and South America. In order to understand the evolution of phosphine resistance and to isolate the responsible genes, we have undertaken genetic linkage analysis of fully sensitive (QRD14), moderately resistant (QRD369) and highly resistant (QRD569) strains of R. dominica collected in Australia. We previously determined that two loci, rph1 and rph2, confer high-level resistance on strain QRD569, which was collected in 1997. We have now confirmed that rph1 is responsible for the moderate resistance of strain QRD369, which was collected in 1990, and is shared with a highly resistant strain from the same geographical region, QRD569. In contrast, rph2 by itself confers only very weak resistance, either as a heterozygote or as a homozygote and was not discovered in the field until weak resistance (probably due to rph1) had become ubiquitous. Thus, high-level resistance against phosphine has evolved via stepwise acquisition of resistance alleles, first at rph1 and thereafter at rph2. The semi-dominance of rph2 together with the synergistic interaction between rph1 and rph2 would have led to rapid selection for homozygosity. A lack of visible fitness cost associated with alleles at either locus suggests that the resistance phenotype will persist in the field.

show abstract

Section: Discussionmentioning

confidence: 91%

Gene interactions constrain the course of evolution of phosphine resistance in the lesser grain borer, Rhyzopertha dominica

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The resistance substitution (glycine 137 to aspartic acid) leads to a gain of organophosphorus hydrolase activity at the expense of the carboxylesterase activity (Newcomb et al 1997;Campbell et al 1998a). A second gene, Rop-2, on chromosome VI, also in£uences resistance to diazinon, but occurs very rarely in natural populations (Arnold & Whitten 1976). Rop-2 codes for a mixed-function oxidase (Hughes & Devonshire 1982;Terras et al 1983).…”

Section: Predicting Resistance Mechanismsmentioning

confidence: 99%

Predicting insecticide resistance: mutagenesis, selection and response

McKenzie

Batterham

1998

Phil. Trans. R. Soc. Lond. B

View full text Add to dashboard Cite

Strategies to manage resistance to a particular insecticide have usually been devised after resistance has evolved. If it were possible to predict likely resistance mechanisms to novel insecticides before they evolved in the ¢eld, it might be feasible to have programmes that manage susceptibility. With this approach in mind, single-gene variants of the Australian sheep blow£y, Lucilia cuprina, resistant to dieldrin, diazinon and malathion, were selected in the laboratory after mutagenesis of susceptible strains. The genetic and molecular bases of resistance in these variants were identical to those that had previously evolved in natural populations. Given this predictive capacity for known resistances, the approach was extended to anticipate possible mechanisms of resistance to cyromazine, an insecticide to which L. cuprina populations remain susceptible after almost 20 years of exposure. Analysis of the laboratory-generated resistant variants provides an explanation for this observation. The variants show low levels of resistance and a selective advantage over susceptibles for only a limited concentration range. These results are discussed in the context of the choice of insecticides for control purposes and of delivery strategies to minimize the evolution of resistance.

show abstract

“…The ROp-1 gene maps to chromosome 4 (20)(21)(22). It encodes a membrane-bound carboxylesterase (E3) of unknown function in susceptible flies (23)(24)(25).…”

Section: Evolution Of Insecticide Resistancementioning

confidence: 99%

Asymmetry – where evolutionary and developmental genetics meet

et al. 1996

View full text Add to dashboard Cite

The mechanisms responsible for the fine tuning of development, where the wildtype phenotype is reproduced with high fidelity, are not well understood. The difficulty in approaching this problem is the identification of mutant phenotypes indicative of a defect in these fine-tuning control mechanisms. Evolutionary biologists have used asymmetry as a measure of developmental homeostasis. The rationale for this was that, since the same genome controls the development of the left and right sides of a bilaterally symmetrical organism, departures from symmetry can be used to measure genetic or environmental perturbations. This paper examines the relationship between asymmetry and resistance to organophosphorous insecticides in the Australian sheep blowfly, Lucilia cuprina. A resistance gene, Rop-1, which encodes a carboxylesterase enzyme, also confers a significant increase in asymmetry. Continued exposure of resistant populations to insecticide has selected a dominant suppressor of the asymmetry phenotype. Genetic evidence indicates that the modifier is the L. cuprina Notch homologue.

show abstract

The genetic basis for organophosphorus resistance in the Australian sheep blowfly, Lucilia cuprina (Wiedemann) (Diptera, Calliphoridae)

Cited by 40 publications

References 23 publications

Gene interactions constrain the course of evolution of phosphine resistance in the lesser grain borer, Rhyzopertha dominica

Gene interactions constrain the course of evolution of phosphine resistance in the lesser grain borer, Rhyzopertha dominica

Predicting insecticide resistance: mutagenesis, selection and response

Asymmetry – where evolutionary and developmental genetics meet

Contact Info

Product

Resources

About