The Genetic, Biochemical, Nutritional and Antimicrobial Characteristics of Pomegranate (Punica granatum L.) Grown in Istria

Abstract

SummaryThis study characterises the genetic variability of local pomegranate (Punica granatum L.) germplasm from the Slovenian and Croatian areas of Istria. The bioactive components and antioxidant and antimicrobial properties of ethanol and water extracts of different parts of pomegranate fruit were also determined, along with their preliminary nutritional characterisation. Twenty-six different genotypes identified with microsatellite analysis indicate the great diversity of pomegranate in Istria. The pomegranate fruit ethanol extracts represent rich sources of phenolic compounds (mean value of the mass fraction in exocarp and mesocarp expressed as gallic acid is 23 and 16 mg/g, respectively). The ethanol extracts of pomegranate exocarp and mesocarp showed the greatest antimicrobial activity against Candida albicans, Candida parapsilosis, Rhodotorula mucilaginosa, Exophiala dermatitidis and Staphylococcus aureus, and the same water extracts against S. aureus and Escherichia coli. To the best of our knowledge, this study represents the first report of the characterisation of pomegranate genetic resources from Istria at different levels, including the molecular, chemical, antimicrobial and nutritional properties.

Traditional Decoction and PUAE Aqueous Extracts of Pomegranate Peels as Potential Low-Cost Anti-Tyrosinase Ingredients

“…It is known that bioactive compounds are variable in pomegranate, and generally in plant material, according to genetic factors, different collection stages, pedoclimatic conditions of the sampling sites (growing wild or cultivation), application of agricultural practices, and post-harvest handling [34,35].…”

Comparative Study between Extracts of Different Pomegranate Parts Issued from Five Tunisian Cultivars (Punica granatum L.): Phytochemical Content, Volatile Composition and Biological Activity

“…Later the antimicrobial activity of extracts of pomegranate peel and juice on cariogenic bacteria has been also highlighted by Gianmaria Fabrizio Ferrazzano (Ferrazzano et al, 2017). Moreover MiklavčičVišnjevec et al, (2017) have reported the ethanol and water extracts of pomegranate exocarp and mesocarp showed the greatest antimicrobial activity against many bacterial strains tested.…”

HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species

“…The HPLC system included a binary pump (model G7112B), Agilent 1260 Autosampler (model G7129A) and a Poroshell 120, EC-C18, 2.1 × 150 mm, 2.7 µm column (693775-902, Agilent Technologies, Santa Clara, CA, USA). The following method for the HPLC-MS analysis rests on our previous studies of phenolic compounds investigation [ 41 , 42 ]. Separation was obtained with a linear gradient of (A) water + 0.1% formic acid ( v / v ) and (B) acetonitrile/methanol (50:50, v / v ), starting at 3.0 % B and increased to 100.0% B in 15 min and held for 5 min (flow rate 0.30 mL/min, column temperature 50 °C, injection volume 1 µL).…”