The genetic characteristics and evolution of Tembusu virus

Lei, Wenwen; Guo, Xin; Fu, Shihong; Feng, Yue; Tao, Xin; Gao, Xiaoyan; Song, Jingdong; Yang, Zhonghua; Zhou, Hongning; Liang, Guodong

doi:10.1016/j.vetmic.2017.01.003

Cited by 39 publications

(47 citation statements)

References 20 publications

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“…Therefore, the monitoring of DTMUV in ducks and other species should be routinely conducted. In addition, time‐scaled Bayesian MCC tree revealed that TMUV first appeared in Asia in 1931, which is consistent to a previous report showing that the recent common ancestor of TMUV might have appeared before 1934 (Lei et al, ). This finding is supported by the first report of mosquito‐derived TMUV in 1955 (Kono et al, ).…”

Section: Discussionsupporting

confidence: 89%

“…Among these proteins, E protein has high variability since it is associated with host cell entry and contains virus‐neutralizing epitopes (Lindenbach, Thiel, & Rice, ; Mukhopadhyay, Kuhn, & Rossmann, ). Therefore, besides the polyprotein gene, the clusters of DTMUV are usually classified based on the genetic variation of the E gene (Dai, Li, & Tao, ; Lei et al, ). Recently, DTMUV has been genetically described into two different clusters, 1 and 2, of which cluster 2 has been further divided into two subclusters (2.1 and 2.2) (Ninvilai et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Genetic characterization of duck Tembusu virus in Thailand, 2015‐2017: Identification of a novel cluster

Ninvilai

Tunterak

Oraveerakul

et al. 2019

Transbound Emerg Dis

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Duck Tembusu virus (DTMUV) infected cases have increasingly been observed in several duck farms in Thailand since its first report in 2013. However, information on the genetic characteristic of DTMUVs recently circulating in ducks in Thailand is limited. In this study, we investigated the geographic distribution and genetic characteristic of DTMUVs recently circulating in ducks in Thailand during 2015-2017. Of the 288 clinical samples obtained from 89 ducks farms located in duck raising areas of Thailand, 65 samples (22.57%) of 34 duck farms (38.20%) were DTMUV positive. Our results demonstrated that DTMUV was extensively distributed in duck raising areas of Thailand. Phylogenetic analysis of the E and NS5 genes revealed that DTMUVs circulating in Thailand were divided into three distinct clusters, including cluster 1, subcluster 2.1 and a novel cluster 3. Among these three clusters, subcluster 2.1 was a predominant cluster of DTMUV circulating in duck populations in Thailand during 2015-2017. It is interesting to note that a novel cluster of DTMUV (cluster 3), which was genetically different from any of the previously reported DTMUV clusters, was first identified in this study. In conclusion, our data demonstrated the circulation of different clusters of DTMUV and the presence of a novel DTMUV cluster in ducks in Thailand. This study highlights the high genetic diversity of DTMUVs in Thailand and the necessity of the routine surveillance of DTMUV for early detection, prevention and control of newly emerging DTMUVs.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Genetic characterization of duck Tembusu virus in Thailand, 2015‐2017: Identification of a novel cluster

Ninvilai

Tunterak

Oraveerakul

et al. 2019

Transbound Emerg Dis

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“…Hemorrhagic ovarian inflammation, also termed egg-drop syndrome, is primarily caused by duck Tembusu virus (DTMUV), and has resulted in significant economic losses in the poultry industry in Southeast Asia since 2010 [1]. DTMUV, similar to other flaviviruses, is a single-stranded, positive-sense RNA virus with an approximately 11 kb genome.…”

Section: Introductionmentioning

confidence: 99%

“…To date, DTMUV-related studies have focused on pathogenicity [21,22], detection methods [23,24], vaccines [10,25,26], transmission routes [27], genetic evolution and epidemiology [1,28,29], and molecular structure [30,31]. Unfortunately, the immune mechanisms of DTMUV invasion and infection in host cells remain poorly understood.…”

Section: Introductionmentioning

confidence: 99%

Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

Lin

Tang

et al. 2018

IJMS

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Duck is a major waterfowl species in China, providing high-economic benefit with a population of up to 20–30 billion per year. Ducks are commonly affected by severe diseases, including egg-drop syndrome caused by duck Tembusu virus (DTMUV). The immune mechanisms against DTMUV invasion and infection remain poorly understood. In this study, duck embryo fibroblasts (DEFs) were infected with DTMUV and harvested at 12 and 24 h post-infection (hpi), and their genomes were sequenced. In total, 911 (764 upregulated and 147 downregulated genes) and 3008 (1791 upregulated and 1217 downregulated) differentially expressed genes (DEGs) were identified at 12 and 24 hpi, respectively. Kyoto Encyclopedia of Genes and Genomes enrichment analysis revealed that DEGs were considerably enriched in immune-relevant pathways, including Toll-like receptor signaling pathway, Cytosolic DNA-sensing pathway, RIG-I-like receptor signaling pathway, Chemokine signaling pathway, NOD-like receptor signaling pathway, and Hematopoietic cell lineage at both time points. The key DEGs in immune system included those of the cytokines (IFN α2, IL-6, IL-8L, IL-12B, CCR7, CCL19, and CCL20), transcription factors or signaling molecules (IRF7, NF-κB, STAT1, TMEM173, and TNFAIP3), pattern recognition receptors (RIG-I and MDA5), and antigen-presenting proteins (CD44 and CD70). This suggests DTMUV infection induces strong proinflammatory/antiviral effects with enormous production of cytokines. However, these cytokines could not protect DEFs against viral attack. Our data revealed valuable transcriptional information regarding DTMUV-infected DEFs, thereby broadening our understanding of the immune response against DTMUV infection; this information might contribute in developing strategies for controlling the prevalence of DTMUV infection.

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“…Variation analyses of amino acid loci in the TMUV E protein revealed two mutated amino acid loci in strains isolated from Malaysia, Thailand, and mainland China compared to the prototypical strain of the virus (MM1775) isolated from mosquitos (26). Furthermore, TMUV isolates from the Chinese mainland have six common variations in the E protein that differ from the Southeast Asian strains (26).…”

mentioning

confidence: 99%

A Single Mutation at Position 156 in the Envelope Protein of Tembusu Virus Is Responsible for Virus Tissue Tropism and Transmissibility in Ducks

Yan

Shi

Wang

et al. 2018

J Virol

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Duck Tembusu virus (TMUV), like other mosquito-borne flaviviruses, such as Japanese encephalitis virus, West Nile virus, and Bagaza virus, is able to transmit vector-independently. To date, why these flaviviruses can be transmitted without mosquito vectors remains poorly understood. To explore the key molecular basis of flavivirus transmissibility, we compared virus replication and transmissibility of an early and a recent TMUV in ducks. The recent TMUV strain FX2010 replicated systemically and transmitted efficiently in ducks, while the replication of early strain MM1775 was limited and did not transmit among ducks. The TMUV envelope protein and its domain I were responsible for tissue tropism and transmissibility. The mutation S156P in the domain I resulted in disruption of N-linked glycosylation at amino acid 154 of the E protein and changed the conformation of "150 loop" of the E protein, which reduced virus replication in lungs and abrogated transmission in ducks. These data indicate that the 156S in the envelope protein is critical for TMUV tissue tropism and transmissibility in ducks in the absence of mosquitos. Our findings provide novel insights on understanding TMUV transmission among ducks. Tembusu virus, similar to other mosquito-borne flaviviruses such as WNV, JEV, and BAGV, can be transmitted without the presence of mosquito vectors. We demonstrate that the envelope protein of TMUV and its amino acid (S) at position 156 is responsible for tissue tropism and transmission in ducks. The mutation S156P results in disruption of N-linked glycosylation at amino acid 154 of the E protein and changes the conformation of "150 loop" of the E protein, which induces limited virus replication in lungs and abrogates transmission between ducks. Our findings provide new knowledge about TMUV transmission among ducks.

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The genetic characteristics and evolution of Tembusu virus

Cited by 39 publications

References 20 publications

Genetic characterization of duck Tembusu virus in Thailand, 2015‐2017: Identification of a novel cluster

Genetic characterization of duck Tembusu virus in Thailand, 2015‐2017: Identification of a novel cluster

Comparative Transcriptomic Analysis of Immune-Related Gene Expression in Duck Embryo Fibroblasts Following Duck Tembusu Virus Infection

A Single Mutation at Position 156 in the Envelope Protein of Tembusu Virus Is Responsible for Virus Tissue Tropism and Transmissibility in Ducks

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