The genetic control of purine biosynthesis in Staphylococcus aureus

Temperature sensitive-salt remedial mutants of Staphylococcus aureus

“…Other strains de rived from 655 were also used. Strain 655C has been cured of a prophage carried by the parent 655 (Gutman and Pattee, 1970), 655N is resistant to the antibiotic novobicin (Pattee and Baldwin, 1961), and strain 655 gua-76 is a guanine requiring auxotroph (Gabbard, 1967).…”

“…19 66), Therefore, the feasibility of obtaining temperature sensitive mutants that formed spheroplasts at the restrictive temperature was investigated. Cells of aureus 655 gua-76 (Gabbard, 1967) were spread on plates of BHI agar (1.05% agar)…”

Temperature sensitive-salt remedial mutants of Staphylococcus aureus

“…19 66), Therefore, the feasibility of obtaining temperature sensitive mutants that formed spheroplasts at the restrictive temperature was investigated. Cells of aureus 655 gua-76 (Gabbard, 1967) were spread on plates of BHI agar (1.05% agar) containing 100 units of penicillin G per ml, and 10% heat-inactivated horse serum. Duplicate plates were incubated at 30C and 43C and examined periodically.…”