The Genetic Determinant of Staphylococcal Penicillinase*

Novick, Richard P.

doi:10.1111/j.1749-6632.1965.tb11637.x

Cited by 6 publications

(1 citation statement)

References 31 publications

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“…However, in only one case is a b-lactam the inducing ligand -in Bacillus spp., a relative of which is also present in Staphylococcus aureus as a horizontally acquired element (Gregory et al, 1997;Novick, 1965;Wilke et al, 2004;Zhang et al, 2001). In the well-characterized AmpR induction system (Hanson & Sanders, 1999), and in the BlrAB induction mechanism characterized above, it is a component of peptidoglycan found at higher levels in b-lactam-challenged cells that is the activatory ligand.…”

Section: Discussionmentioning

confidence: 99%

Induction of -lactamase production in Aeromonas hydrophila is responsive to -lactam-mediated changes in peptidoglycan composition

et al. 2010

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We have studied the mechanism by which b-lactam challenge leads to b-lactamase induction in Aeromonas hydrophila through transposon-insertion mutagenesis. Disruption of the DDcarboxypeptidases/endopeptidases, penicillin-binding protein 4 or BlrY leads to elevated monomer-disaccharide-pentapeptide levels in A. hydrophila peptidoglycan and concomitant overproduction of b-lactamase through activation of the BlrAB two-component regulatory system. During b-lactam challenge, monomer-disaccharide-pentapeptide levels increase proportionately with b-lactamase production and b-lactamase induction is inhibited by vancomycin, which binds muro-pentapeptides. Taken together, these data strongly suggest that the Aeromonas spp. blactamase regulatory sensor kinase, BlrB, responds to the concentration of monomerdisaccharide-pentapeptide in peptidoglycan.

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