The genetic mating system of spotted sunfish (<i>Lepomis punctatus</i>): mate numbers and the influence of male reproductive parasites

DeWoody, J. Andrew; Fletcher, Dean E.; Mackiewicz, Mark; Wilkins, Selwyn; Avise, John C.

doi:10.1046/j.1365-294x.2000.01123.x

Cited by 32 publications

(33 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These components of reproductive success for males using alternative tactics should not be affected by only selection, but also are likely to vary as a function of factors such as overall male density, relative frequency of males using alternative tactics in a population, and habitat type. Published studies of sperm competition in fish, for example, have already revealed that sperm competition can be intense (22) when multiple male matings occur; however, the frequency of such matings in nature is known for only a few species (10,11,29). As predicted by our model, an increased incidence of multiple male matings should act to delay a Trojan gene effect.…”

Section: Resultsmentioning

confidence: 61%

“…After fertilization, eggs were collected from each female and reared until hatching. Medaka fry were then collected, preserved in 100% ethanol, and subsequently dissected to remove the eyes (which often inhibit PCR); their DNA was extracted according to conventional procedures (11). For the parentage analyses, a tetranucleotide microsatellite locus (designated OL1) was developed from a medaka sequence found in the GenBank database (accession no.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Transgenic male mating advantage provides opportunity for Trojan gene effect in a fish

Howard

DeWoody²,

Muir³

2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Genetically modified (GM) strains now exist for many organisms, producing significant promise for agricultural production. However, if these organisms have some fitness advantage, they may also pose an environmental harm when released. High mating success of GM males relative to WT males provides such an important fitness advantage. Here, we provide documentation that GM male medaka fish modified with salmon growth hormone possess an overwhelming mating advantage. GM medaka offspring possess a survival disadvantage relative to WT, however. When both of these fitness components are included in our model, the transgene is predicted to spread if GM individuals enter wild populations (because of the mating advantage) and ultimately lead to population extinction (because of the viability disadvantage). Mating trials indicate that WT males use alternative mating tactics in an effort to counter the mating advantage of GM males, and we use genetic markers to ascertain the success of these alternative strategies. Finally, we model the impact of alternative mating tactics by WT males on transgene spread. Such tactics may reduce the rate of transgene spread, but not the outcome.genetically modified organism ͉ alternative mating tactics ͉ sperm competition ͉ medaka T he production of genetically modified (GM) organisms (GMOs) continues at a rapid pace, prompting concerns about undesirable ecological consequences if these organisms enter natural communities (1-3). Based on six major components of an organism's fitness (i.e., juvenile viability, adult viability, age at sexual maturity, female fecundity, male fertility, and male mating advantage), we have recently provided a framework to predict possible risks of ecological harm associated with the spread of transgenes after a GMO release. One risk, extinction, results in the local elimination of conspecific populations (both WT and GM individuals); the other risk, invasion, involves ecosystem disruption as GM individuals replace their WT counterparts (4, 5).Harm to wild populations resulting from either extinction or invasion risk requires that transgenes of GMOs can spread in nature, which in turn requires that GMOs have an advantage over their WT counterparts in at least one fitness component. An extinction risk is predicted when a transgene produces conflicting effects on different fitness components. As a result of an advantage in one component, GMOs replace WT genotypes in a naturally occurring population of conspecifics, whereas a disadvantage in another fitness component reduces population size, ultimately resulting in population extinction. We refer to this scenario as a Trojan gene effect (4, 5).One scenario in which opposing pleiotropic effects of transgenes are predicted to produce a Trojan gene effect is when GM males have a mating advantage relative to WT males, but the GM offspring they produce have reduced viability relative to WT offspring (4, 5). The predicted extinction outcome resulting from these opposing effects has recently been confirmed with a determin...

show abstract

Section: Resultsmentioning

confidence: 61%

Section: Methodsmentioning

confidence: 99%

Transgenic male mating advantage provides opportunity for Trojan gene effect in a fish

Howard

DeWoody²,

Muir³

2004

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…For example, DeWoody et al . (1998) demonstrated that sneaker males in the redbreast sunfish ( Lepomis auritus ) were parental males with their own nests, and that sneaking generally yielded low reproductive pay‐offs in this species as well as in the spotted sunfish ( Lepomis punctatus ) (DeWoody et al . 2000a).…”

Section: Introductionmentioning

confidence: 99%

A molecular genetic examination of the mating system of pumpkinseed sunfish reveals high pay‐offs for specialized sneakers

Ríos-Cardenas

Webster

2008

Molecular Ecology

View full text Add to dashboard Cite

Intrasexual variation in reproductive behaviour and morphology are common in nature. Often, such variation appears to result from conditional strategies in which some individuals (e.g. younger males or those in poor condition) adopt a low pay-off phenotype as a 'best of a bad job'. Alternatively, reproductive polymorphisms can be maintained by balancing selection, with male phenotypes having equal fitnesses at equilibrium, but examples from nature are rare. Many species of sunfish (genus Lepomis) are thought to have alternative male reproductive behaviours, but most empirical work has focused on the bluegill sunfish and the mating systems of other sunfish remain poorly understood. We studied a population of pumpkinseed sunfish (Lepomis gibbosus) in upstate New York. Field observations confirm the existence of two male reproductive strategies: 'parentals' were relatively old and large males that maintained nests, and 'sneakers' were relatively young and small males that fertilize eggs by darting into nests of parentals during spawning. The sneaker and parental male strategies appear to be distinct life-history trajectories. Sneaker males represented 39% of the males observed spawning, and sneakers intruded on 43% of all mating attempts. Microsatellite analyses revealed that sneaker males fertilized an average of 15% of the eggs within a nest. This level of paternity by sneaker males appears to be higher than seen in most other fishes, and preliminary analyses suggest that the two male reproductive strategies are maintained as a balanced polymorphism.

show abstract

“…Brood parasitism has been detected by paternity analysis in a range of fishes, including cichlids (Dierkes et al 1999;Taborsky 2001), darters (DeWoody et al 2000a), gobies (DeWoody et al 2000bJones et al 2001), and sunfish (DeWoody et al 1998, 2000c. Although brood parasitism may most commonly take the form of male sneaking, genetic analysis has also been used to detect egg thievery, which is known to occur in some species (Largiadèr et al 2001).…”

Section: Breeding Systems and Parental Carementioning

confidence: 99%

Molecular pedigree analysis in natural populations of fishes: approaches, applications, and practical considerations

Wilson¹,

Ferguson²

2002

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Molecular markers can provide information on the family structure of natural fish populations through molecular pedigree analysis. This information, which is otherwise difficult to obtain, can give important insights into the expression and evolution of phenotypic traits. We review the literature to provide examples of how molecular pedigree analysis has been used extensively to examine patterns of distribution, dispersal, and social behaviour in fishes and how it provides a tool for the estimation of quantitative genetic parameters. Although multiple methodologies can be used to examine family structure, the efficacy of any molecular pedigree analysis is generally dependent on prior consideration of interrelated statistical and biological factors. Statistical issues stem from the choice of molecular marker type and marker set used, in addition to sampling strategy. We discuss these considerations and additionally emphasize the utility of supplemental nongenetic data for increasing the efficacy of pedigree analysis. We advocate that, where possible, a priori knowledge of the study system's biology should be used to inform study design and further highlight the need for additional empirical testing of methodologies.

show abstract

The genetic mating system of spotted sunfish (Lepomis punctatus): mate numbers and the influence of male reproductive parasites

Cited by 32 publications

References 43 publications

Transgenic male mating advantage provides opportunity for Trojan gene effect in a fish

Transgenic male mating advantage provides opportunity for Trojan gene effect in a fish

A molecular genetic examination of the mating system of pumpkinseed sunfish reveals high pay‐offs for specialized sneakers

Molecular pedigree analysis in natural populations of fishes: approaches, applications, and practical considerations

Contact Info

Product

Resources

About