The Genome Analysis of Blomia tropicalis Reveals A Comprehensive Allergen Profile

Xiong, Qing; Wan, Tsz; Malainual, Nat; Tsui, Stephen Kwok-Wing

doi:10.1016/j.jaci.2019.12.682

Cited by 4 publications

(7 citation statements)

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“…Its completeness was estimated at 95%, with 9.1% duplicated genes based on BUSCO with the Arachnida dataset. In contrast, a previously generated and annotated transcriptome included fewer predicted genes (16,590–14,899) 58,59 …”

Section: Resultsmentioning

confidence: 97%

“…In contrast, a previously generated and annotated transcriptome included fewer predicted genes (16,590–14,899). 58 , 59 …”

Section: Resultsmentioning

confidence: 99%

“…In contrast, a previously generated and annotated transcriptome included fewer predicted genes (16,590-14,899). 58,59 The proteome analyses identified 3714 protein hits in four protein sample types individually collected from adult mites; pools of mites of different ages, including eggs; water extracts of feces; and detergent-buffer extracts of the remaining pellets of the water extracts.…”

Section: Genome Transcriptome and Proteome Of B Tropicalismentioning

confidence: 99%

“…However, we identified the AAQ24542 protein in the proteome 58,59 (Table 1; Supplementary material Figures S5-S6). Isoform AAQ24542 was not detected in transcripts.…”

Section: Identification Of Known Allergens and Putative Allergens In ...mentioning

confidence: 99%

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Predicting Blomia tropicalis allergens using a multiomics approach

Hubert,

Vrtala,

Sopko

et al. 2023

Clinical & Translational All

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BackgroundThe domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite has not been sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis thus far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by the World Health Organization (WHO)/IUIS in domestic astigmatid mites suggest the presence of a large set of additional allergens.MethodsHere, we employ a multiomics approach to assess the allergome of B. tropicalis using genomic and transcriptomic sequence data and perform highly sensitive protein abundance quantification.FindingsAmong the 14 known allergen groups, we confirmed 13 (one WHO/IUIS allergen, Blo t 19, was not found) and identified 16 potentially novel allergens based on sequence similarity. These data indicate that B. tropicalis shares 27 known/deduced allergen groups with pyroglyphid house dust mites (genus Dermatophagoides). Among these groups, five allergen‐encoding genes are highly expressed at the transcript level: Blo t 1, Blo t 5, Blo t 21 (known), Blo t 15, and Blo t 18 (predicted). However, at the protein level, a different set of most abundant allergens was found: Blo t 2, 10, 11, 20 and 21 (mite bodies) or Blo t 3, 4, 6 and predicted Blo t 13, 14 and 36 (mite feces).InterpretationWe report the use of an integrated omics method to identify and predict an array of mite allergens and advanced, label‐free proteomics to determine allergen protein abundance. Our research identifies a large set of novel putative allergens and shows that the expression levels of allergen‐encoding genes may not be strictly correlated with the actual allergenic protein abundance in mite bodies.

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Section: Resultsmentioning

confidence: 97%

“…In contrast, a previously generated and annotated transcriptome included fewer predicted genes (16,590–14,899). 58 , 59 …”

Section: Resultsmentioning

confidence: 99%

Section: Genome Transcriptome and Proteome Of B Tropicalismentioning

confidence: 99%

“…However, we identified the AAQ24542 protein in the proteome 58,59 (Table 1; Supplementary material Figures S5-S6). Isoform AAQ24542 was not detected in transcripts.…”

Section: Identification Of Known Allergens and Putative Allergens In ...mentioning

confidence: 99%

See 2 more Smart Citations

Predicting Blomia tropicalis allergens using a multiomics approach

Hubert,

Vrtala,

Sopko

et al. 2023

Clinical & Translational All

View full text Add to dashboard Cite

show abstract

“…A BUSCO analysis with the Arachnida database estimated its completeness to be 95%, with 9.1% gene duplication. In contrast, a previous transcriptome had 16,590/14,899 predicted genes and 5,310 KEGG pathways (Xiong et al 2020 , 2022 ). We identified 433 KEGG pathways and 68 modules (Table 1 ) in the transcriptome-level analyses.…”

Section: Resultsmentioning

confidence: 98%

Mixta mediterraneensis as a novel and abundant gut symbiont of the allergen-producing domestic mite Blomia tropicalis

Erban,

Sopko,

Klimov

et al. 2024

Exp Appl Acarol

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Blomia tropicalis is an allergen-producing mite in the human environment in tropical regions. The microbiome of B. tropicalis was described using the barcode sequencing region of V4 16S rDNA and genome assemblage. Mixta mediterraneensis, previously isolated from human skin swabs, was identified as a B. tropicalis gut symbiont based on genome assembly. The microbiome contains two bacteria, Staphylococcus and M. mediterraneensis. The number of M. mediterraneensis 16S DNA copies was 106 per mite and 109 per feces in the rearing chamber based on qPCR quantification. The profile of this bacterium reached 50% of reads in the mite gut and feces. Genomic analyses revealed that the bacterium has several metabolic pathways that suggest metabolic cooperation with the mite host in vitamin and amino acid synthesis, nitrogen recycling, and antimicrobial defense. Lysozyme is present in the symbiotic bacterium but absent in the mite. The B. tropicalis microbiome contained Staphylococcus, which accelerates mite population growth. Mites can digest Staphylococcus by using specific enzymes with hydrolytic functions against bacterial cell walls (chitinases and cathepsin D), leading to endocytosis of bacteria and their degradation in lysosomes and phagosomes. Gene expression analysis of B. tropicalis indicated that phagocytosis was mediated by the PI3-kinase/Akt pathway interacting with the invasins produced by M. mediterraneensis. Moreover, the symbiont had metabolic pathways that allowed it to recycle the mite metabolic waste product guanine, known as a mite attractant. The mite host symbiont enhances mite aggregation in the feces, and the fecal–oral transmission route is excepted.

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PredictingBlomia tropicalisallergens using a multiomics approach

Hubert,

Vrtala,

Sopko

et al. 2023

Preprint

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The domestic mite Blomia tropicalis is a major source of allergens in tropical and subtropical regions. Despite its great medical importance, the allergome of this mite is not sufficiently studied. Only 14 allergen groups have been identified in B. tropicalis so far, even though early radioimmunoelectrophoresis techniques (27 uncharacterized allergen complexes) and comparative data based on 40 allergen groups officially recognized by WHO/IUIS in domestic astigmatid mites, suggest that a large set of additional allergens may be present. Here, we use a multiomics approach to assess the allergome of B. tropicalis using genomic, transcriptomic sequence data, and a highly sensitive protein abundance quantification. Out of 14 known allergen groups, we confirmed 13 (one WHO/IUIS allergen, Blo t 19, was found to be a contaminant originated from a nematode) and identified 16 potentially novel allergens using sequence similarity. These data indicate that B. tropicalis shares 27 known/deduced allergen groups with pyroglyphid house dust mites (genus Dermatophagoides). Among them, five allergen-encoding genes were highly expressed at the transcript level: Blo t 1, Blo t 5, Blo t 21 (known), Blo t 15, and Blo t 18 (predicted). However, at the protein level, a different set of most abundant allergens was found: Blo t 2, 10, 11, 20 and 21 (mite bodies) or Blo t 3, 4, 6 and predicted Blo t 13, 14 and 36 (mite feces). We show the use of an integrated omics method to identify and predict an array of mite allergens and advanced, label-free proteomics to determine allergen protein abundance. Our research identifies a large set of novel putative allergens and shows that expression levels of allergen-encoding genes may not be strictly correlated with the actual allergenic protein abundance in the mite bodies.

show abstract

The Genome Analysis of Blomia tropicalis Reveals A Comprehensive Allergen Profile

Cited by 4 publications

References 0 publications

Predicting Blomia tropicalis allergens using a multiomics approach

Predicting Blomia tropicalis allergens using a multiomics approach

Mixta mediterraneensis as a novel and abundant gut symbiont of the allergen-producing domestic mite Blomia tropicalis

PredictingBlomia tropicalisallergens using a multiomics approach

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