The genome and the antigen gene repertoire of Trypanosoma brucei gambiense are smaller than those of T. b. brucei

Dero, B; Zampetti‐Bosseler, F.; Pays, Étienne; Steinert, Maurice

doi:10.1016/0166-6851(87)90077-6

Cited by 25 publications

(15 citation statements)

References 30 publications

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“…The complement of variant surface glycoproteins in T. b. gambiense has previously been reported as being smaller, or more restricted, than that of T. b. brucei, based on a smaller overall genome size and reduced subtelomeric components [8]. This could indicate fewer discrete VSG sequence types or just fewer copies of an equal number of types.…”

Section: Resultsmentioning

confidence: 95%

“…T. b. gambiense comprises two groups; a genetically homogeneous group to which the majority of isolates belong (group 1), and a second represented by a handful of isolates from West Africa (group 2). Group 1 T. b. gambiense strains have the smallest genomes in the T. brucei species complex, having 71–82% of the highest DNA content measured for T. b. brucei [7]–[8]. Pulse-field gel analysis of T. b. gambiense chromosomes shows that few if any mini-chromosomes are present compared to the estimated 100 in T. b. brucei and T. b. rhodesiense , and the mini-chromosomes are also of a smaller size–average 25 kb in T. b. gambiense compared to 100 kb in T. b. brucei and T. b. rhodesiense [8]–[9].…”

Section: Introductionmentioning

confidence: 99%

“…Perhaps as a consequence of this reduced genome, T. b. gambiense also has a restricted repertoire of Variant Surface Glycoprotein (VSG) genes [8], [10]–[12]. At any time, bloodstream form trypanosomes possess a surface glycoprotein coat formed through the expression of a single gene from a large archive of VSGs [13].…”

Section: Introductionmentioning

confidence: 99%

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The Genome Sequence of Trypanosoma brucei gambiense, Causative Agent of Chronic Human African Trypanosomiasis

et al. 2010

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Background Trypanosoma brucei gambiense is the causative agent of chronic Human African Trypanosomiasis or sleeping sickness, a disease endemic across often poor and rural areas of Western and Central Africa. We have previously published the genome sequence of a T. b. brucei isolate, and have now employed a comparative genomics approach to understand the scale of genomic variation between T. b. gambiense and the reference genome. We sought to identify features that were uniquely associated with T. b. gambiense and its ability to infect humans.Methods and FindingsAn improved high-quality draft genome sequence for the group 1 T. b. gambiense DAL 972 isolate was produced using a whole-genome shotgun strategy. Comparison with T. b. brucei showed that sequence identity averages 99.2% in coding regions, and gene order is largely collinear. However, variation associated with segmental duplications and tandem gene arrays suggests some reduction of functional repertoire in T. b. gambiense DAL 972. A comparison of the variant surface glycoproteins (VSG) in T. b. brucei with all T. b. gambiense sequence reads showed that the essential structural repertoire of VSG domains is conserved across T. brucei.ConclusionsThis study provides the first estimate of intraspecific genomic variation within T. brucei, and so has important consequences for future population genomics studies. We have shown that the T. b. gambiense genome corresponds closely with the reference, which should therefore be an effective scaffold for any T. brucei genome sequence data. As VSG repertoire is also well conserved, it may be feasible to describe the total diversity of variant antigens. While we describe several as yet uncharacterized gene families with predicted cell surface roles that were expanded in number in T. b. brucei, no T. b. gambiense-specific gene was identified outside of the subtelomeres that could explain the ability to infect humans.

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Section: Resultsmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

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The Genome Sequence of Trypanosoma brucei gambiense, Causative Agent of Chronic Human African Trypanosomiasis

et al. 2010

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“…The majority of T. b. gambiense isolates form a homogeneous group (group 1) that stands apart from the rest of the T. brucei group, because of its restricted range of genetic polymorphisms and limited antigenic repertoire [20-24]. It is clear that there is a much greater genetic distance between T. b. gambiense group 1 and other T. brucei subspecies than between T. b. brucei and T. b. rhodesiense .…”

Section: Subspecies Of Trypanosoma Bruceimentioning

confidence: 99%

Species concepts for trypanosomes: from morphological to molecular definitions?

Gibson

2003

Kinetoplastid Biol Dis

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The way species and subspecies names are applied in African trypanosomes of subgenera Trypanozoon and Nannomonas is reviewed in the light of data from molecular taxonomy. In subgenus Trypanozoon the taxonomic importance of pathogenicity, host range and distribution appear to have been inflated relative to actual levels of genetic divergence. The opposite is true for subgenus Nannomonas, where current taxonomic usage badly underrepresents genetic diversity. Data from molecular characterisation studies are revealing a growing number of genotypes, which may represent distinct taxa. Unfortunately few of these genotypes are yet supported by sufficient biological data to be recognized taxonomically. But we may be missing fundamental epidemiological information, because of our inability to distinguish these trypanosomes in host blood morphologically or in tsetse by their developmental cycle. Molecular taxonomy has led the way in identifying these new genotypes and now offers the key to elucidating the biology of these organisms.

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“…Les gènes responsables de la synthèse de ces glycopro téines sont connus (Dero et al, 1987). L'activation succes sive de parties du génome détermine la séquence des variants observés.…”

Section: Moyens Techniques Actuelsunclassified

Les lents progrès du contrôle de la maladie du sommeil

Wéry¹

1990

Ann. Parasitol. Hum. Comp.

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The genome and the antigen gene repertoire of Trypanosoma brucei gambiense are smaller than those of T. b. brucei

Cited by 25 publications

References 30 publications

The Genome Sequence of Trypanosoma brucei gambiense, Causative Agent of Chronic Human African Trypanosomiasis

The Genome Sequence of Trypanosoma brucei gambiense, Causative Agent of Chronic Human African Trypanosomiasis

Species concepts for trypanosomes: from morphological to molecular definitions?

Les lents progrès du contrôle de la maladie du sommeil

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