The Genome of Cronobacter sakazakii Bacteriophage vB_CsaP_GAP227 Suggests a New Genus within the
            <i>Autographivirinae</i>

Abbasifar, Reza; Kropinski, Andrew M.; Sabour, Parviz M.; Ackermann, Hans-Wolfgang; Villa, Argentina Alanis; Abbasifar, Arash; Griffiths, Mansel W.

doi:10.1128/genomea.00122-12

Cited by 11 publications

(8 citation statements)

References 17 publications

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“…In the BLASTN results, JG068 shows similarity to several members of the Autographivirinae subfamily and ϕKMV-like phages genus, including ϕRSB1, Cd1, and Pseudomonas phage Bf7. Members of this subfamily (with enterobacteria phage T7 being the best characterized) belong to one of several genera, including the T7-like phages, SP6-like phages, ϕKMV-like phages, and novel genera that have not yet been named [35,36]. These podoviruses all encode a single subunit RNA polymerase, as is found in JG068 [35].…”

Section: Resultsmentioning

confidence: 99%

Genomic characterization of JG068, a novel virulent podovirus active against Burkholderia cenocepacia

et al. 2013

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BackgroundAs is true for many other antibiotic-resistant Gram-negative pathogens, members of the Burkholderia cepacia complex (BCC) are currently being assessed for their susceptibility to phage therapy as an antimicrobial treatment. The objective of this study was to perform genomic and limited functional characterization of the novel BCC phage JG068 (vB_BceP_JG068).ResultsJG068 is a podovirus that forms large, clear plaques on Burkholderia cenocepacia K56-2. Host range analysis indicates that this phage can infect environmental, clinical, and epidemic isolates of Burkholderia multivorans, B. cenocepacia, Burkholderia stabilis, and Burkholderia dolosa, likely through interaction with the host lipopolysaccharide as a receptor. The JG068 chromosome is 41,604 base pairs (bp) in length and is flanked by 216 bp short direct terminal repeats. Gene expression originates from both host and phage promoters and is in the forward direction for all 49 open reading frames. The genome sequence shows similarity to Ralstonia phage ϕRSB1, Caulobacter phage Cd1, and uncharacterized genetic loci of blood disease bacterium R229 and Burkholderia pseudomallei 1710b. CoreGenesUniqueGenes analysis indicates that JG068 belongs to the Autographivirinae subfamily and ϕKMV-like phages genus. Modules within the genome encode proteins involved in DNA-binding, morphogenesis, and lysis, but none associated with pathogenicity or lysogeny. Similar to the signal-arrest-release (SAR) endolysin of ϕKMV, inducible expression of the JG068 SAR endolysin causes lysis of Escherichia coli that is dependent on the presence of an N-terminal signal sequence. In an in vivo assay using the Galleria mellonella infection model, treatment of B. cenocepacia K56-2-infected larvae with JG068 results in a significant increase in larval survival.ConclusionsAs JG068 has a broad host range, does not encode virulence factors, is obligately lytic, and has activity against an epidemic B. cenocepacia strain in vivo, this phage is a highly promising candidate for BCC phage therapy development.

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Section: Resultsmentioning

confidence: 99%

Genomic characterization of JG068, a novel virulent podovirus active against Burkholderia cenocepacia

et al. 2013

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“…As shown earlier, based on phylogenetic analysis, Pectobacterium phage PP2 and its homologous Cronobacter bacteriophages vB_CskP_GAP227 [21] and Dev-CD-23823 form an unclassified group within the subfamily Autographivirinae [19]. To clarify the evolutionary origin of Arno 160 and to find other members of this group, we conducted phylogenetic studies of amino acid sequences of five conservative genes (DNA polymerase I, major capsid protein, DNA-directed RNA polymerase, head-to-tail connector protein and a large subunit of terminase (DNA maturase B)) and their concatenated alignments.…”

Section: Taxonomymentioning

confidence: 89%

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Shneider

Lukianova

Evseev

et al. 2020

IJMS

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Phytopathogenic bacteria belonging to the Pectobacterium and Dickeya genera (soft-rot Pectobacteriaceae) are in the focus of agriculture-related microbiology because of their diversity, their substantial negative impact on the production of potatoes and vegetables, and the prospects of bacteriophage applications for disease control. Because of numerous amendments in the taxonomy of P. carotovorum, there are still a few studied sequenced strains among this species. The present work reports on the isolation and characterization of the phage infectious to the type strain of P. carotovorum. The phage Arno 160 is a lytic Podovirus representing a potential new genus of the subfamily Autographivirinae. It recognizes O-polysaccahride of the host strain and depolymerizes it in the process of infection using a rhamnosidase hydrolytic mechanism. Despite the narrow host range of this phage, it is suitable for phage control application.

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“…For example, Pectobacterium carotovorum subsp. carotovorum phage PP2 possesses homology to Cronobacter phage vB_CsaP_Gap277, a phage that has been identified to represent a distinct genus within Autographivirinae [ 62 , 63 ].…”

Section: Discussionmentioning

confidence: 99%

Pectobacterium atrosepticum Phage vB_PatP_CB5: A Member of the Proposed Genus ‘Phimunavirus’

Buttimer

Lucid

Neve

et al. 2018

Viruses

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Pectobacterium atrosepticum is a phytopathogen of economic importance as it is the causative agent of potato blackleg and soft rot. Here we describe the Pectobacterium phage vB_PatP_CB5 (abbreviated as CB5), which specifically infects the bacterium. The bacteriophage is characterized in detail and TEM micrographs indicate that it belongs to the Podoviridae family. CB5 shares significant pairwise nucleotide identity (≥80%) with P. atrosepticum phages φM1, Peat1, and PP90 and also shares common genome organization. Phylograms constructed using conserved proteins and whole-genome comparison-based amino acid sequences show that these phages form a distinct clade within the Autographivirinae. They also possess conserved RNA polymerase recognition and specificity loop sequences. Their lysis cassette resembles that of KP34virus, containing in sequential order a U-spanin, a holin, and a signal–arrest–release (SAR) endolysin. However, they share low pairwise nucleotide identity with the type phage of the KP34virus genus, Klebsiella phage KP34. In addition, phage KP34 does not possess several conserved proteins associated with these P. atrosepticum phages. As such, we propose the allocation of phages CB5, Peat1, φM1, and PP90 to a separate new genus designated Phimunavirus.

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The Genome of Cronobacter sakazakii Bacteriophage vB_CsaP_GAP227 Suggests a New Genus within the Autographivirinae

Cited by 11 publications

References 17 publications

Genomic characterization of JG068, a novel virulent podovirus active against Burkholderia cenocepacia

Genomic characterization of JG068, a novel virulent podovirus active against Burkholderia cenocepacia

Autographivirinae Bacteriophage Arno 160 Infects Pectobacterium carotovorum via Depolymerization of the Bacterial O-Polysaccharide

Pectobacterium atrosepticum Phage vB_PatP_CB5: A Member of the Proposed Genus ‘Phimunavirus’

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