The Genome Sequence of Brucella intermedia DF13, a 2,4-Dichlorophenoxyacetic Acid-Degrading Soil Bacterium Isolated in Brazil

Silva, Sheila da; Peckle, Barbara Alvarenga; Ribeiro, José Roberto de Assis; Oliveira, Selma Soares de; Bianco, Kayo; Clementino, Maysa M.; Direito, Ida Carolina Neves; Macrae, Andrew

doi:10.1128/mra.01105-21

Microbiol Resour Announc

2022

DOI: 10.1128/mra.01105-21

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The Genome Sequence of Brucella intermedia DF13, a 2,4-Dichlorophenoxyacetic Acid-Degrading Soil Bacterium Isolated in Brazil

Sheila da Silva

Barbara Alvarenga Peckle

José Roberto de Assis Ribeiro

et al.

Abstract: Brucella intermedia / Ochrobactrum intermedium strain DF13 was isolated from Brazilian soil and is able to degrade 2,4-dichlorophenoxyacetic acid (2,4-D). Here, we report on its genome sequence, with 4,570,268 bp and a 57.8% G+C content.

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2024

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Unveiling six novel bacterial strains for fipronil and thiobencarb biodegradation: efficacy, metabolic pathways, and bioaugmentation potential in paddy soil

Faridy,

Torabi,

Pourbabaee

et al. 2024

Front. Microbiol.

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IntroductionSoil bacteria offer a promising approach to bioremediate pesticide contamination in agricultural ecosystems. This study investigated the potential of bacteria isolated from rice paddy soil for bioremediating fipronil and thiobencarb, common agricultural pesticides.MethodsBacterial isolates capable of degrading fipronil and thiobencarb were enriched in a mineral salt medium. A response surface methodology with a Box-Behnken design was utilized to optimize pesticide degradation with the isolated bacteria. Bioaugmentation tests were performed in paddy soils with varying conditions.Results and discussionSix strains, including single isolates and their mixture, efficiently degraded these pesticides at high concentrations (up to 800 µg/mL). Enterobacter sp., Brucella sp. (alone and combined), and a mixture of Stenotrophomonas sp., Bordetella sp., and Citrobacter sp. effectively degraded fipronil and thiobencarb, respectively. Notably, a single Pseudomonas sp. strain degraded a mixture of both pesticides. Optimal degradation conditions were identified as a slightly acidic pH (6-7), moderate pesticide concentrations (20-50 µg/mL), and a specific inoculum size. Bioaugmentation assays in real-world paddy soils (sterile/non-sterile, varying moisture) demonstrated that these bacteria significantly increased degradation rates (up to 14.15-fold for fipronil and 5.13-fold for thiobencarb). The study identifies these novel bacterial strains as promising tools for bioremediation and bioaugmentation strategies to tackle fipronil and thiobencarb contamination in paddy ecosystems.

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Unveiling six novel bacterial strains for fipronil and thiobencarb biodegradation: efficacy, metabolic pathways, and bioaugmentation potential in paddy soil

Faridy,

Torabi,

Pourbabaee

et al. 2024

Front. Microbiol.

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show abstract

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The Genome Sequence of Brucella intermedia DF13, a 2,4-Dichlorophenoxyacetic Acid-Degrading Soil Bacterium Isolated in Brazil

Abstract: Brucella intermedia / Ochrobactrum intermedium strain DF13 was isolated from Brazilian soil and is able to degrade 2,4-dichlorophenoxyacetic acid (2,4-D). Here, we report on its genome sequence, with 4,570,268 bp and a 57.8% G+C content.

Cited by 1 publication

References 19 publications

Unveiling six novel bacterial strains for fipronil and thiobencarb biodegradation: efficacy, metabolic pathways, and bioaugmentation potential in paddy soil

Unveiling six novel bacterial strains for fipronil and thiobencarb biodegradation: efficacy, metabolic pathways, and bioaugmentation potential in paddy soil

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