The genome sequence of the soft-rot fungus<i>Penicillium purpurogenum</i>reveals a high gene dosage for lignocellulolytic enzymes

Mardones, Wladimir; Génova, Alex Di; Cortés, María Paz; Travisany, Dante; Maass, Alejandro; Eyzaguirre, Jaime

doi:10.1080/21501203.2017.1419995

Cited by 12 publications

(3 citation statements)

References 76 publications

(67 reference statements)

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“…Furthermore, Camassol and Dillon (2009) reported maximum xylanase activity of 9.95 ± 1.53 U/gds of bagasse and wheat bran in combination under solid state fermentation using Penicillium echinulatum. Apart from optimizing strategies, for the enhanced production of hemicellulolytic enzymes, cloning and expression studies have also been carried out to increase the potential enzyme secretion for lignocellulose degradation to valued products (Mardones et al, 2018;Echeverria and Eyzaguirre, 2019).…”

Section: Discussionmentioning

confidence: 99%

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

2020

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Corn cob is an abundant organic source with significant potential in sustainable energy development. For the effective conversion of the feedstocks to valued commodities, effective biocatalysts are highly desired. The present study aims at optimizing the critical parameters required for xylanase production by Penicillium purpurogenum isolated from rotten wood sample using the Taguchi orthogonal array layout of L25 (5 ∧ 6). The optimized conditions like temperature 40 • C, pH 3, size of inoculum 1.2 × 10 8 spores/ml, moisture 70%, peptone 0.8%, and 5 days of incubation resulted in 1,097 ± 6.76 U/gram dry substrate (gds) xylanase which was 65.72% more when compared to un-optimized production of xylanase. The xylanase thus produced, effectively carried out pretreated corn cob saccharification and the reaction was further improved with ultrasound assistance which has increased the saccharification yield to 12.02% along with significant reduction in reaction time. The saccharification efficiency of pretreated corn cob was found to be 80.29% more compared to the raw corn cob, reflecting its recalcitrance to digestion. Indeed, xylan being the second most abundant polymer in lignocellulosic biomass, considerable attention is being paid for its effective conversion to valued products.

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Section: Discussionmentioning

confidence: 99%

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

2020

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“…Next-generation sequencing of the P. citreonigrum strain IMI92228 yielded a total of 7611 Mbp, and the final assembly was found to contain 27 Mbp in 79 scaffolds. The total length of scaffolds and estimated genome size were relatively small compared to previously reported genomes of the Penicillium species [24][25][26][27], although these scaffolds code 97.3% of single copy genes that were conserved in Eurotiomycetes, which is enough to search for the homologous gene cluster that corresponds to the CTV biosynthetic cluster of A. terreus. The estimated genome size may be underestimated because only short inset sizes were estimated, and short read analysis may be insufficient to evaluate repeat regions and transposable elements of the genome.…”

Section: Discussionmentioning

confidence: 86%

Whole Genome Analysis Revealed the Genes Responsible for Citreoviridin Biosynthesis in Penicillium citreonigrum

Okano

Kobayashi

Izawa

et al. 2020

Toxins

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Citreoviridin (CTV) is a mycotoxin that is produced by Aspergillus terreus, Eupenicillium ochrosalmoneum and Penicillium citreonigrum, and CTV has been detected in a wide range of cereal grains throughout the world. Furthermore, it is especially a serious problem in regions where rice is consumed as a staple food. Moreover, CTV is a well-known yellow rice toxin, and outbreaks of beriberi have occurred due to consumption of rice that is contaminated by CTV even in the recent years. Although CTV biosynthetic genes of A. terreus have been described, those of P. citreonigrum remain unclear, which is concerning since P. citreonigrum is the main cause of CTV contamination in rice. In the present study, we determined the draft genome of the P. citreonigrum strain IMI92228 and revealed the presence of all four genes that form a gene cluster and that are homologous to the CTV biosynthesis genes of A. terreus. The expression of these four homologous genes were highly correlated with CTV production, suggesting that they may play an important role in CTV biosynthesis in P. citreonigrum. We concluded that the gene cluster is a CTV biosynthesis cluster of P. citreonigrum. The findings will contribute to the understanding of the biosynthetic pathway of CTV and will ultimately lead to improvements in the CTV management of agricultural products.

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“…The composition of AP wheat straw was analyzed by sequential fractionation. In this process, the contents of cellulose, hemicellulose, and Klason lignin were determined through a quantitative acid hydrolysis [ 17 ]. In brief, the solid residue (300 mg) was treated for 1 h with 3 mL of 13.5 M sulfuric acid (H 2 SO 4 ) at 30 °C and 150 rpm.…”

Section: Methodsmentioning

confidence: 99%

Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization

Méndez-Líter

Eugenio

Hakalin

et al. 2021

JoF

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As β-glucosidases represent the major bottleneck for the industrial degradation of plant biomass, great efforts are being devoted to discover both novel and robust versions of these enzymes, as well as to develop efficient and inexpensive ways to produce them. In this work, raw glycerol from chemical production of biodiesel was tested as carbon source for the fungus Talaromyces amestolkiae with the aim of producing enzyme β-glucosidase-enriched cocktails. Approximately 11 U/mL β-glucosidase was detected in these cultures, constituting the major cellulolytic activity. Proteomic analysis showed BGL-3 as the most abundant protein and the main β-glucosidase. This crude enzyme was successfully used to supplement a basal commercial cellulolytic cocktail (Celluclast 1.5 L) for saccharification of pretreated wheat straw, corroborating that even hardly exploitable industrial wastes, such as glycerol, can be used as secondary raw materials to produce valuable enzymatic preparations in a framework of the circular economy.

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The genome sequence of the soft-rot fungusPenicillium purpurogenumreveals a high gene dosage for lignocellulolytic enzymes

Cited by 12 publications

References 76 publications

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

Whole Genome Analysis Revealed the Genes Responsible for Citreoviridin Biosynthesis in Penicillium citreonigrum

Production of a β-Glucosidase-Rich Cocktail from Talaromyces amestolkiae Using Raw Glycerol: Its Role for Lignocellulose Waste Valorization

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