The genome sequence of the model ascomycete fungus Podospora anserina

Espagne, Eric; Lespinet, Olivier; Malagnac, Fabienne; Silva, Corinne Da; Jaillon, Olivier; Porcel, Betina M.; Couloux, Arnaud; Aury, Jean‐Marc; Ségurens, Béatrice; Poulain, Julie; Anthouard, Véronique; Grossetête, Sandrine; Khalili, Hamid; Coppin, Evelyne; Dequard-Chablat, Michelle; Picard, Marguerite; Contamine, Véronique; Arnaise, Sylvie; Bourdais, A; Berteaux‐Lecellier, Véronique; Gautheret, Daniel; Vries, Ronald P. de; Battaglia, Evy; Coutinho, Pedro M.; Danchin, Étienne; Henrissat, Bernard; Khoury, Riyad El; Sainsard-Chanet, Annie; Boivin, Antoine; Pinan‐Lucarré, Bérangère; Sellem, Carole H.; Debuchy, Robert; Wincker, Patrick; Weissenbach, Jean; Silar, Philippe

doi:10.1186/gb-2008-9-5-r77

Cited by 282 publications

(337 citation statements)

References 143 publications

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“…The most highly expressed genes encode proteins predicted to be involved in the metabolism of plant cell wall polysaccharides, many of which were identified by MS analyses. The genomes of filamentous fungi have a large number of predicted glycosyl hydrolases (Ϸ200) with varying numbers of predicted cellulases, from 10 in H. jecorina (11) to 60 in Podospora anserina (20). A comparison between our results and a cDNA expression/Northern analysis of 8 endoglucanases and 7 GH3/␤-glucosidases in H. jecorina (21) showed complete overlap with our profiling data, with the exception of one ortholog of a ␤-glucosidase (cel3e ϭ NCU05577).…”

Section: Discussionmentioning

confidence: 84%

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa

Tian

Beeson

Iavarone

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

286

347

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The filamentous fungus Neurospora crassa is a model laboratory organism, but in nature is commonly found growing on dead plant material, particularly grasses. Using functional genomics resources available for N. crassa, which include a near-full genome deletion strain set and whole genome microarrays, we undertook a systemwide analysis of plant cell wall and cellulose degradation. We identified approximately 770 genes that showed expression differences when N. crassa was cultured on ground Miscanthus stems as a sole carbon source. An overlap set of 114 genes was identified from expression analysis of N. crassa grown on pure cellulose. Functional annotation of up-regulated genes showed enrichment for proteins predicted to be involved in plant cell wall degradation, but also many genes encoding proteins of unknown function. As a complement to expression data, the secretome associated with N. crassa growth on Miscanthus and cellulose was determined using a shotgun proteomics approach. Over 50 proteins were identified, including 10 of the 23 predicted N. crassa cellulases. Strains containing deletions in genes encoding 16 proteins detected in both the microarray and mass spectrometry experiments were analyzed for phenotypic changes during growth on crystalline cellulose and for cellulase activity. While growth of some of the deletion strains on cellulose was severely diminished, other deletion strains produced higher levels of extracellular proteins that showed increased cellulase activity. These results show that the powerful tools available in N. crassa allow for a comprehensive system level understanding of plant cell wall degradation mechanisms used by a ubiquitous filamentous fungus.cellulase ͉ secretome ͉ transcriptome

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Section: Discussionmentioning

confidence: 84%

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa

Tian

Beeson

Iavarone

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

286

347

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“…Taking advantage of the genome sequence of P. anserina (Espagne et al 2008) and of the characterization on each of the seven chromosomes of marker polymorphisms between the geographic strains P. anserina and P. comata, segregation analysis of gene rse2 with the markers linked to gene ura5 was undertaken. A total of 198 monocaryotic spores derived from the cross rse2-1 (anserina) 3 rse2 1 (comata) were generated.…”

Section: Resultsmentioning

confidence: 99%

Mutations in Two Zinc-Cluster Proteins Activate Alternative Respiratory and Gluconeogenic Pathways and Restore Senescence in Long-Lived Respiratory Mutants ofPodospora anserina

et al. 2009

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In Podospora anserina, inactivation of the respiratory chain results in a spectacular life-span extension. This inactivation is accompanied by the induction of the alternative oxidase. Although the functional value of this response is evident, the mechanism behind it is far from understood. By screening suppressors able to reduce the life-span extension of cytochrome-deficient mutants, we identified mutations in two zinc-cluster proteins, RSE2 and RSE3, which are conserved in other ascomycetes. These mutations led to the overexpression of the genes encoding the alternative oxidase and the gluconeogenic enzymes, fructose-1, 6 biphosphatase, and pyruvate carboxykinase. Both RSE2 and RSE3 are required for the expression of these genes. We also show that, even in the absence of a respiratory deficiency, the wild-type RSE2 and RSE3 transcription factors are involved in life-span control and their inactivation retards aging. These data are discussed with respect to aging, the regulation of the alternative oxidase, and carbon metabolism.

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“…broad.mit.edu/annotation/fgi/), The DOE Joint Genome Institute (JGI:http://www.jgi.doe.gov/), Génolevures (http:/ /cbi.labri.fr/Genolevures/) and the Institut de Génétique et Microbiologie-Université de Paris-Sud XI/CNRS (Espagne et al 2008). All downloads were performed before 1 December 2008.…”

Section: Sequence Analysismentioning

confidence: 99%

Evolution of carbonic anhydrases in fungi

Elleuche

Pöggeler

2009

Curr Genet

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The genome sequence of the model ascomycete fungus Podospora anserina

Abstract: Background: The dung-inhabiting ascomycete fungus Podospora anserina is a model used to study various aspects of eukaryotic and fungal biology, such as ageing, prions and sexual development.

Cited by 282 publications

References 143 publications

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa

Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa

Mutations in Two Zinc-Cluster Proteins Activate Alternative Respiratory and Gluconeogenic Pathways and Restore Senescence in Long-Lived Respiratory Mutants ofPodospora anserina

Evolution of carbonic anhydrases in fungi

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