The genome sequence of the cinnamon sedge caddisfly, Limnephilus marmoratus (Curtis, 1834)

Abstract

We present a genome assembly from an individual Limnephilus marmoratus (a caddisfly; Arthropoda; Insecta; Trichoptera; Limnephilidae). The genome sequence is 1,630 megabases in span. Most of the assembly (99.93%) is scaffolded into 30 chromosomal pseudomolecules, including the assembled Z sex chromosome. The mitochondrial genome has also been assembled and is 15.4 kilobases in length.

Characterization of the primary structure of the major silk gene, h-fibroin, across caddisfly (Trichoptera) suborders

“… 980/n.a. C:91.4%[S:89.0%,D:2.4%],F:6.0%,M:2.6% Limnephilus lunatus (DtoL) 42 Integripalpia (tube case) GCA_917563855.2 1,269,651,477 18,993,099/95,392,806 139/39 C:89.7%[S:88.9%,D:0.8%],F:7.4%,M:2.9% Limnephilus marmoratus (DtoL) 43 Integripalpia (tube case) GCA_917880885.1 1,629,971,709 8,018,677/56,174,236 395/68 C:90.4%[S:89.3%,D:1.1%],F:6.7%,M:2.9% Limnephilus rhombicus (DtoL) Integripalpia (tube case) GCA_929108145.2 1,578,808,083 10,796,652/54,234,467 272/62 C:89.8%[S:88.6%,D:1.2%],F:7.1%,M:3.1% Leptonema lineaticorne a Annulipalpia (retreat) GCA_024500535.1 273,010,349 13,827,090/n.a. 65/n.a.…”

“…In this study, we report eight new full-length h-fibroin sequences for caddisflies across a diverse set of silk usage. Two of these were generated from new genomic resources, whereas five were mined from previously published genomes, 41 , 42 , 43 , 44 highlighting the relevance of genome sequencing projects for the wider scientific community. The new full-length h-fibroins represent a substantial increase in the number of genomic resources available for the study of caddisfly silk and allowed us to compare the major silk gene for eleven species across the primary clades of Trichoptera, in which species exhibit different silk usage.…”

“… 73 https://multiqc.info/ R v4.0.3 R Core Team 2021 https://www.r-project.org/contributors.html BlobTools v1.1.1 Laetsch et al. 43 https://github.com/DRL/blobtools blastn 2.10.0+ Camacho et al. 74 https://www.ncbi.nlm.nih.gov/books/NBK279690/ Geneious Prime 2022.1.1 NA https://www.geneious.com/prime/ Augustus v.3.3.3 Hoff and Stanke 73 https://bioinf.uni-greifswald.de/augustus/ SignalP 6.0 Teufel et al.…”